Priority-based multiple products from microalgae: review on techniques and strategies

Sarkar, Sambit; Manna, Mriganka Sekhar; Bhowmick, Tridib Kumar; Gayen, Kalyan

doi:10.1080/07388551.2020.1753649

Cited by 46 publications

(16 citation statements)

References 107 publications

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“…HPLC was performed on an LC-20 chromatograph (Shimadzu, Kyoto, Japan), eluting protein concentrate samples in a sodium chloride concentration gradient. Detection was carried out using a diode array detector in the detection range of 180–900 nm, the flow rate of the eluent in all cases was one mL/min, elution was carried out in a gradient mode, the time and gradient were selected individually for each case of separation, the mixture of treated water (MQ purification level) and acetonitrile with the addition of 0.1% trifluoroacetic acid was used as solvents, separation was carried out on a reversed-phase Phenomenex column (Torrance, CA, USA) 250 mm × 2.5 mm, particle size 25 μm, sorbent was silica gel modified C-18, with phenyl end-capping [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins

et al. 2021

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Microalgae are known to be rich in protein. In this study, we aim to investigate methods of producing and purifying proteins of 98 microalgae including Chlorella vulgaris, Arthrospira platensis, Nostoc sp., Dunaliella salina, and Pleurochrysis carterae (Baltic Sea). Therefore, we studied their amino acid composition and developed a two-stage protein concentrate purification method from the microalgae biomass. After an additional stage of purification, the mass fraction of protein substances with a molecular weight greater than 50 kDa in the protein concentrate isolated from the biomass of the microalga Dunaliella salina increased by 2.58 times as compared with the mass fraction before filtration. In the protein concentrate isolated from the biomass of the microalga Pleurochrysis cartera, the relative content of the fraction with a molecular weight greater than 50.0 kDa reached 82.4%, which was 2.43 times higher than the relative content of the same fractions in the protein concentrate isolated from this culture before the two-stage purification. The possibilities of large-scale industrial production of microalgae biomass and an expanded range of uses determine the need to search for highly productive protein strains of microalgae and to optimize the conditions for isolating amino acids from them.

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Section: Methodsmentioning

confidence: 99%

Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins

et al. 2021

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“…The selection of specific extraction strategy to be followed for each of these metabolites mainly depends on their chemical nature [194]. In recent years, the algal bioactive metabolites are extracted by using the combination of various green-extraction methods [195] which includes: supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), ultrasound-assisted extraction, and microwave-assisted extraction than the conventional methods that requires more time, use of great quantities of organic solvents and also laborious [196,197]. The principle of SFE is the use of critical points of temperature and pressure of the solvent used for extraction, and the primary solvent is used is the supercritical carbon dioxide and sometimes a cosolvent such as small amount of ethanol for more polar compounds.…”

Section: Extraction Strategiesmentioning

confidence: 99%

Identification and characterization of the novel bioactive compounds from microalgae and cyanobacteria for pharmaceutical and nutraceutical applications

et al. 2022

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Microalgae and cyanobacteria (blue‐green algae) are used as food by humans. They have gained a lot of attention in recent years because of their potential applications in biotechnology. Microalgae and cyanobacteria are good sources of many valuable compounds, including important biologically active compounds with antiviral, antibacterial, antifungal, and anticancer activities. Under optimal growth condition and stress factors, algal biomass produce varieties of potential bioactive compounds. In the current review, bioactive compounds production and their remarkable applications such as pharmaceutical and nutraceutical applications along with processes involved in identification and characterization of the novel bioactive compounds are discussed. Comprehensive knowledge about the exploration, extraction, screening, and trading of bioactive products from microalgae and cyanobacteria and their pharmaceutical and other applications will open up new avenues for drug discovery and bioprospecting.

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“…Further, metabolites obtained from microalgae can be used in biofertilizer production as a source of nitrogenand phosphorous-rich biomass residues as feedstock and in the bioenergy industry as bulk oil and biomass residue feedstock for jet fuel, biodiesel, bioethanol, biogas, biochar, and biohydrogen production. Furthermore, some microalgae strains can be used in wastewater treatment by reducing the amount of nitrogen, phosphate, and chemical oxygen demand, as well as removing heavy metals (copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn)) and pharmaceutical pollutants (triclosan and hormones (17β-estradiol and 17α-ethinylestradiol) [108][109][110][111][112][113]. Interestingly, potential industrial applications and commercialization of microalgae-derived biomass and bioactive compounds in the food industry has recently been explored by Camacho et al (2019).…”

Section: Algal Biotechnology In Pharmaceutical Applicationsmentioning

confidence: 99%

Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae

et al. 2022

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During the last two decades, microalgae have attracted increasing interest, both commercially and scientifically. Commercial potential involves utilizing valuable natural compounds, including carotenoids, polysaccharides, and polyunsaturated fatty acids, which are widely applicable in food, biofuel, and pharmaceutical industries. Conversely, scientific potential focuses on bioreactors for producing recombinant proteins and developing viable technologies to significantly increase the yield and harvest periods. Here, viral-based vectors and transient expression strategies have significantly contributed to improving plant biotechnology. We present an updated outlook covering microalgal biotechnology for pharmaceutical application, transformation techniques for generating recombinant proteins, and genetic engineering tactics for viral-based vector construction. Challenges in industrial application are also discussed.

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Priority-based multiple products from microalgae: review on techniques and strategies

Cited by 46 publications

References 107 publications

Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins

Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins

Identification and characterization of the novel bioactive compounds from microalgae and cyanobacteria for pharmaceutical and nutraceutical applications

Current Status and Perspective on the Use of Viral-Based Vectors in Eukaryotic Microalgae

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