Pristine C60 Fullerene Nanoparticles Ameliorate Hyperglycemia-Induced Disturbances via Modulation of Apoptosis and Autophagy Flux

Demir, Ersin; Nedzvetsky, V. S.; Ağca, Can Ali; Kırıcı, Mahinur

doi:10.1007/s11064-020-03097-w

Cited by 23 publications

(17 citation statements)

References 83 publications

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“…Антиоксидантні властивості фулерену С60 можуть бути корисними при взаємодії з компонентами оливкової олії. Однак фулерен С60 може активувати внутрішньоклітинні шляхи регуляції метаболізму та вести до колапсу протилежно спрямованих клітинних реакцій на життєво критичні сигнали, зокрема, коливання вмісту глюкози та продукції інсуліну [9]. На додаток, фулерен С60 спроможний модулювати прозапальну відповідь різних типів клітин і тим самим стимулювати надмірну клітинну відповідь на зовнішні стимули [32,33,36].…”

Section: вступunclassified

Influence of potential nanopollutant fullerene C60 on physiological and biochemical responses in mammals

Nedzvetsky

Gasso

Yermolenko

et al. 2021

vsllr

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The progressive development of technologies in the manufacture and application of nanomaterials in almost all spheres of human life causes penetration in an organism and accumulation of nanoparticles in its cells. Determinations of the risk of using nanomaterials and mechanisms of their cytotoxicity are extremely relevant current problems that should be studied. Fullerene C60 is the most widespread nanomaterial proposed to use inhibition of tumour growth, microbial infections, and purposeful drug delivery. However, there are contradictory data on cytotoxic and/or cytoprotective effects of this fullerene. In the present paper, the action of fullerene C60 on glucose metabolism, the composition of the intestinal microbiota, and an acid-reducing balance were studied in rats. It is shown that fullerene C60 dissolved in olive oil (2 mg/kg/day) induces insulin resistance, activates the peroxidation of lipids in the brain of animals, but not in the liver, under conditions of chronic influence. In addition, fullerene C60 induced changes in the composition of the intestinal microbiota in rats. Determined disorders may be a cause of insulin production, as an adaptive response to the needs of metabolic energy under local oxidative stress in the nerve tissue. At the same time, the growth of insulin resistance can be induced by nonspecific molecular damage in biomembranes and macromolecules, including insulin receptors. In this regard, the explanation of the molecular mechanisms of insulin resistance induced by fullerene C60 together with the effect of fullerene dose will be of particular interest in further studies.

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Section: вступunclassified

Influence of potential nanopollutant fullerene C60 on physiological and biochemical responses in mammals

Nedzvetsky

Gasso

Yermolenko

et al. 2021

vsllr

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“…Various anticancer effects of several fullerene forms were established including DNA methylation [13], cell cycle arrest [14], antiangiogenic effect [15] and stress-induced apoptosis [16]. Besides, neuroprotective effect of pristine C60 fullerene on autophagy flux and apoptosis was demonstrated in rat brain stressed with hyperglycemia [17]. One of the hallmarks of malignation is the overproduction of reactive oxygen and nitrogen species (ROS/RNS) that play an importante role in cancer process [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…There is a rising evidence that prevaling number, if not all, of various cellular disturbances are accompanied by increased free radicals generation. Despite wide studied pro-oxidant effects of functionalized fullerene, pristine C60 fullerene could inhibit tumor progress via modulation of regulatory pathways and gene expression [17,23]. Glioblastoma cells progress depends on multiple mutations and abnormal regulation of cell death pathways, including resistance to programmed cell death initiation [24].…”

Section: Introductionmentioning

confidence: 99%

Hydrated C60 fullerene, glioblastoma hücre hattında parthanatosu arttırır ve otofaji ile ilgili biyobelirteçleri indükler

Faraj

Nedzvetsky

Tykhomyrov

et al. 2022

Türk Doğa Ve Fen Dergisi

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Glioblastoma, kemo ve radyoterapiye karşı dirençli, en agresif beyin kanseri tiplerinden biridir. C60 fulleren türevi nanopartiküller, çeşitli modellerde antikanser aktivite amacı ile geliştirilmektedir. Birçok kemoterapi ajanının aksine, bu fulleren çeşitli konsantrasyonlarda toksik değildir. C60 fulleren, birçok biyomedikal uygulama için umut verici bir adaydır. Bu nedenle, suda çözünür hydrated C60 fullerene'in (HyC60Fn) insan glioblastoma U373 hücresinde PARP, Beclin1, LC3 ve GFAP ekspresyonu üzerindeki etkileri araştırılmıştır. Hücre canlılığı ve göçü, sırasıyla MTT ve yara iyileşmesi testi ile belirlendi. PARP, Beclin1 ve LC3 ekspresyonu western blot ile ve GFAP ise immünositokimya ile tespit edildi. 0.5 – 2.0 µM doz aralığındaki HyC60Fn, doza bağlı bir şekilde hücre canlılığını azalttığı belirlendi. Ayrıca, HyC60Fn 1.0 ve 2.0 µM dozları, glioblastoma hücre göçünü belirgin şekilde bastırmıştır. Mekanizma olarak, HyC60Fn'nin otofaji belirteçleri olarak Beclin-1'i ve LC3-II/LC3-I ekspresyon oranını belirgin şekilde yukarı regüle ettiği belirlendi. Ayrıca, suda çözünür HyC60Fn’nin PARP fragmanı ve bu durumun doğal sonuç olarak glioblastoma U373 hücrelerinde parthanatos aktive ettiği belirlendi. Mevcut sonuçlar, HyC60Fn'nin, glioblastoma hücrelerinde şiddetli otofaji akışı ve parthanatos kombinasyonu yoluyla anti-tümör etkisini başlatabildiğini göstermektedir. Bu nedenle HyC60Fn, glioblastoma hücrelerinin reaktivitesini ve programlanmış hücre ölümünü modüle ederek en azından kısmen hücre ölüm mekanizmasını etkiler. Bulgularımız, HyC60Fn 'in umut verici bir kanser karşıtı terapötik olabileceğini ve bu konuda daha fazla çalışmanın gerekli olduğunu göstermektedir.

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“…The use of fullerene C60, as a new therapeutic approach, is being intensely developed by exploring cellular signaling pathways. In recent years, fullerene C60 and its derivatives have exhibited various beneficial bioactivities, including anti-bacterial [1], anti-viral [2], anti-inflammatory [3], neuroprotective [4], autophagic [5], antitumor [6], and anti-aging [7]. Previous research has found that fullerene C60 specifically binds to and activates the hippocampal Ca2+ signaling protein (CaMKIIα) to increase learning and memory [8].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the use of fullerene C60 for tumor treatment has been reported, where it enhances the antitumor effect by mediating CaMKIIα activity [6]. Several other studies have documented remarkable antitumor effects of fullerenes via a variety of mechanisms involving immunomodulation, autophagy modulation, and oxidative stress [5,9,10]. Excess free radicals and oxidative stress are generally known to be detrimental to human health [11], and several diseases have been associated with increased levels of oxidative stress, including cancer, Alzheimer's disease, Parkinson's disease, inflammatory disorders, and asthma [6,12,13].…”

Section: Introductionmentioning

confidence: 99%

Fullerene C60 Protects Against Intestinal Injury from Deoxynivalenol Toxicity by Improving Antioxidant Capacity

Liao

Liu

Tan

et al. 2021

Life

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Oxidative stress is involved in a wide variety of pathologies, and fullerene has been shown to have an antioxidant ability. Mycotoxins exert toxic effects through induction of excessive reactive oxygen species (ROS). Here, we evaluated water-soluble fullerene C60 for its anti-mycotoxin and antioxidant effects in vitro and in vivo. Intestinal epithelial cells were cultured with fullerene during deoxynivalenol (DON) exposure. The results revealed that fullerene C60 significantly promoted cell viability, decreased apoptosis and necrotic cell number, and significantly reduced intracellular ROS levels during DON exposure (p < 0.05). To investigate the role of fullerene in antioxidant capacity in vivo further, 40 three-week-old male C57BL/6 mice were randomly divided into four groups. There were no significant differences between the control and fullerene groups (p > 0.05). In mice exposed to DON, supplementation with fullerene C60 significantly improved growth performance, and enhanced the total antioxidant status and the activities of SOD and GPX in the intestine and liver (p < 0.05). In addition, fullerene C60 supplementation improved intestinal morphology, as indicated by a higher villus height and tight junction protein expression (p < 0.05). Furthermore, fullerene supplementation decreased serum concentrations of inflammatory cytokine and lipopolysaccharide (LPS; a penetrability marker) compared to the DON-challenged group (p < 0.05). The current study suggests that fullerene C60 improves intestinal antioxidant status against DON-induced oxidative stress in vitro and in vivo.

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Pristine C60 Fullerene Nanoparticles Ameliorate Hyperglycemia-Induced Disturbances via Modulation of Apoptosis and Autophagy Flux

Cited by 23 publications

References 83 publications

Influence of potential nanopollutant fullerene C60 on physiological and biochemical responses in mammals

Influence of potential nanopollutant fullerene C60 on physiological and biochemical responses in mammals

Hydrated C60 fullerene, glioblastoma hücre hattında parthanatosu arttırır ve otofaji ile ilgili biyobelirteçleri indükler

Fullerene C60 Protects Against Intestinal Injury from Deoxynivalenol Toxicity by Improving Antioxidant Capacity

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