Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

Zhang, Yi; Hu, Guoqing; Li, Shang; Li, Zhen Hua; Lam, Che Oi; Hong, Si Jia; Kwan, Yiu Wa; Chan, Shun‐Wan; Leung, George Pak-Heng; Lee, Simon Ming‐Yuen

doi:10.3892/mmr.2011.716

Cited by 22 publications

(15 citation statements)

References 17 publications

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“…Several studies have reported stimulatory effects of Astragaloside (main compound extracted from AM ) [ 4 , 22 ] on cell proliferation, which was also observed in this study. Leydig cells influence sexual maturation of males by secreting testosterone, which is influenced by cell number and rate of steroidogenesis.…”

Section: Discussionsupporting

confidence: 85%

“…Astragalus membranaceus ( AM ), a well-known Chinese medicinal plant, has been widely used as a traditional prescription medicine for treatment of various diseases, including hepatitis [ 1 ], diabetic nephropathy [ 2 , 3 ], cardiovascular disorders [ 4 , 5 ] and skin diseases [ 6 ]. AM has various favorable pharmacological effects including anti-fibrotic [ 1 , 7 – 9 ], anti-oxidant [ 10 ], anti-apoptotic [ 11 ], anti-inflammatory [ 12 ] and immune-enhancing properties [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Jiang

Cao

Huang

et al. 2015

BMC Complement Altern Med

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BackgroundAstragalus membranaceus (AM) is a Chinese traditional herb which has been reported to have broad positive effects on many diseases, including hepatitis, heart disease, diabetes and skin disease. AM can promote cell proliferation, increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and inhibit apoptosis by regulating the transcription of proto-oncogenes controlling cell death. While AM is included in some commercially available “testosterone boosting supplements”, studies directly testing ability of AM to modulate testosterone production are lacking. In the present study, we examined the effects of AM on Leydig cell function in vitro.MethodsRat Leydig cells were purified and treated with AM at different concentrations (0 μg/mL, 10 μg/mL, 20 μg/mL, 50 μg/mL, 100 μg/mL and 150 μg/mL) and cell counting-8 (CCK-8) assay, Enzyme-linked immunosorbent assay, quantitative real time PCR and analysis of activities of SOD and GPx were done respectively.ResultsTreatment with 100 μg/mL (P < 0.05) and 150 μg/mL AM (P < 0.01) significantly increased Leydig cell numbers. Treatment with AM (20 μg/mL, 50 μg/mL and 100 μg/mL) significantly increased testosterone production (P < 0.01). In addition, increased Leydig cell SOD and GPx activities were observed in response to 20 μg/mL and 50 μg/mL AM treatment (P < 0.01). Furthermore, expression of Bax mRNA was significantly decreased (P < 0.01), and the ratio of Bcl-2/Bax mRNA was significantly increased in response to 20 μg/mL AM in the culture medium (P < 0.05).ConclusionsResults supported a beneficial effect of AM on multiple aspects of rat Leydig cell function in vitro including testosterone production.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Jiang

Cao

Huang

et al. 2015

BMC Complement Altern Med

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“…During decade, several researches have focused on studying extraction separation, pharmacokinetics, and pharmacological activities of astragaloside IV by separation and pure. It has been reported that astragaloside IV can promote zebrafish in vivo , which is closely associated with increase in the expression of vascular endothelial cell growth factor and its receptor, thereby activating the pathway of protein kinase B and phosphoinositide 3-kinase, as well as regulation of the expression of hypoxia inducible factor protein [ 17 ]. With regard to protection of endothelial function, researches showed that astragaloside IV can resist lipoprotein-induced injury to endothelial cells and increase the level of malondialdehyde (MDA) and SOD [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Astragaloside IV on the SDF-1/CXCR4 Expression in Atherosclerosis of apoE^−/−Mice Induced by Hyperlipaemia

Qin

Liu

Lin

2015

Evidence-Based Complementary and Alternative Medicine

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Astragaloside IV (AsIV) is the major effective component extracted from the Chinese herb Astragalus membranaceus, which has been widely used to treat cardiovascular disease. Recent studies have shown that AsIV can potentially protect the arteries from atherosclerosis; however the mechanisms underneath are unknown. The aim of this study was to investigate the effects of AsIV on blood lipids, CD40-CD40L signal system, and SDF-1/CXCR4 biological axis in high-fat diet apoE−/− mice and reveal the molecular mechanisms of AsIV against atherosclerosis. Here, we showed that AsIV alleviated the extent of atherosclerosis in aorta of apoE−/− mice. And AsIV can significantly downregulate PAC-1, CD40L, and CXCR4 expression on platelet surface in blood of high-fat diet apoE−/− mice. AsIV also can significantly downregulate mRNA and protein level of SDF-1 and CXCR4 in thoracic aorta. Consistent with aorta CXCR4 expression, CXCR4 in bone marrow-derived endothelial progenitor cell (EPC) was also reduced. Meanwhile biochemical analysis showed that AsIV could downregulate TG, TC, and LDL-C levels and upregulate HDL-C level in blood of high-fat diet apoE−/− mice. We concluded that the protective effects of AsIV in atherosclerosis injury may be related to regulating blood lipids, CD40-CD40L system, and SDF-1/CXCR4 biological axis. SDF-1/CXCR4 biological axis is probably one of the main targets of intervening atherosclerosis.

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“…In addition, PDGF-BB has been reported to induce an increase in VSMC migration (23). Since AS-IV has been found to play a protective role in the cardiovascular system (2), AS-IV was hypothesized to exhibit a suppressive effect on the PDGF-BB-induced upregulation of VSMC migration. The results demonstrated that following PDGF-BB treatment, the migration of HDVSMCs was significantly upregulated, together with increased expression levels of MMP2 and MMP9, which is consistent with the results of previous studies (24,25).…”

Section: Discussionmentioning

confidence: 99%

“…Astragaloside IV (AS-IV) is the main compound extracted from the astragalus root, and has been recently demonstrated to exhibit various pharmacological effects on the liver, nervous system, hematopoietic system, endocrine system, cardiac function, metabolism of collagen and organ immune system (1). Furthermore, AS-IV has been shown to promote the proliferation of human umbilical vein endothelial cells, as well as the formation of tube-like structures in vitro (2). In addition, AS-IV has been reported to stimulate angiogenesis via the phosphoinositide 3-kinase/Akt, janus kinase 2/signal transducer and activator of transcription 3 and extracellular-signal-regulated kinase 1/2 pathways (3,4).…”

Section: Introductionmentioning

confidence: 99%

Astragaloside IV inhibits platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells via the inhibition of p38 MAPK signaling

Chen

Cai

Zhang

et al. 2014

Experimental and Therapeutic Medicine

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Astragaloside IV (AS-IV), the major active component extracted from Astragalus membranaceus, has been demonstrated to exhibit protective effects on the cardiovascular, immune, digestive and nervous systems; thus, has been widely used in traditional Chinese medicine. Abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is closely associated with the initiation and progression of cardiovascular diseases, including atherosclerosis and restenosis. However, the effects of AS-IV on VSMCs remain unknown. For the first time, the present study demonstrated that AS-IV markedly suppressed platelet-derived growth factor (PDGF)-BB-stimulated cellular proliferation and migration of HDMEC-a human dermal VSMCs (HDVSMCs). Further investigation into the underlying molecular mechanisms demonstrated that the administration of AS-IV attenuated the PDGF-BB-stimulated switch of HDVSMCs into a proliferative phenotype. Furthermore, AS-IV inhibited the PDGF-BB-induced expression of cell cycle-associated proteins, as well as the upregulation of matrix metalloproteinase (MMP)2, but not MMP9. In addition, AS-IV was shown to downregulate the activation of p38 mitogen-activated protein kinase (MAPK) signaling induced by PDGF-BB in HDVSMCs. Therefore, the observations of the present study indicate that AS-IV inhibits PDGF-BB-stimulated VSMC proliferation and migration, possibly by inhibiting the activation of the p38 MAPK signaling pathway. Thus, AS-IV may be useful for the treatment of vascular diseases.

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Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

Cited by 22 publications

References 17 publications

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Effects of Astragaloside IV on the SDF-1/CXCR4 Expression in Atherosclerosis of apoE^−/−Mice Induced by Hyperlipaemia

Astragaloside IV inhibits platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells via the inhibition of p38 MAPK signaling

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Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

Cited by 22 publications

References 17 publications

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Effects of treatment with Astragalus Membranaceus on function of rat leydig cells

Effects of Astragaloside IV on the SDF-1/CXCR4 Expression in Atherosclerosis of apoE−/−Mice Induced by Hyperlipaemia

Astragaloside IV inhibits platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells via the inhibition of p38 MAPK signaling

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Effects of Astragaloside IV on the SDF-1/CXCR4 Expression in Atherosclerosis of apoE^−/−Mice Induced by Hyperlipaemia