Pro-apoptotic and size-reducing effects of protein corona-modulating nano-architectures enclosing platinum prodrug in in vivo oral carcinoma

Abstract

The selective and localized delivery of active agents to neoplasms is crucial to enhance the chemotherapeutic efficacy while reducing the associated side effects. The encapsulation of chemotherapeutics in nanoparticles decorated...

Hybrid nano-architectures loaded with metal complexes for the co-chemotherapy of head and neck carcinomas

“…The nano-architectures have been synthesized by employing standard protocols with some modifications due to the encapsulation of the ruthenium complex (NAs-RuCy) and of both the metallic drugs (NAs-CisPt-RuCy). 19 A schematic explanation of the steps involved in the synthesis is reported in Fig. 1A.…”

“…All nanoparticles were formulated starting from a standard protocol for the synthesis of NAs as described previously. 19 Briefly, ultrasmall gold nanoparticles with a diameter of approximately 3 nm were prepared by adding 20 mL of poly(sodium 4-styrene sulfonate) (PSS, 30% aqueous solution) and 200 mL of HAuCl 4 aqueous solution (10 mg mL À1 ) to 20 mL of milliQ s water. During stirring, 200 mL of sodium borohydride (8 mg mL À1 in milliQ water) was added quickly to the mixture that was then mixed for another 2 minutes.…”

“…NAs are hybrid nano-architectures composed of biodegradable silica capsules containing a polymeric matrix and ultrasmall metal nanoparticles. 19 The extreme versatility of the synthetic protocol allows for the loading of active molecules in the inner cavity as well as for surface functionalization. 20,21 The NA family has been developed to avoid the issue of plasmon nanomaterial persistence after the action with a special focus on oncology.…”

Drug-Free Hybrid Nanoarchitecture Modulation of the Metastatic Behavior of Pancreatic Ductal Adenocarcinoma in Alternative in Vivo Models

“…14,33 The silica shell protects the inner core and provides a modifiable surface for active targeting purposes. 38 The synthetic protocol is standardized with a high batch-by-batch homogeneity, and its versatility is demonstrated by the possibility to include various metals (gold, silver, platinum, copper) and/or active molecules or dyes. 39,40 As we have already demonstrated, plasmon USMNPs enclosed in NAs are efficiently excreted after intravenous injection or intranasal instillation in healthy mice.…”

“…The polymeric matrix is formed by polymers of opposite charge, namely, poly­(sodium 4-styrenesulfonate) (PPS, negatively charged) and poly­( l -lysine) (PL, positively charged). The polymeric templates enclosing USMNPs are embedded in a silica capsule formed via a modified Stöber reaction (final diameter: ∼150 nm). , The silica shell protects the inner core and provides a modifiable surface for active targeting purposes . The synthetic protocol is standardized with a high batch-by-batch homogeneity, and its versatility is demonstrated by the possibility to include various metals (gold, silver, platinum, copper) and/or active molecules or dyes. , As we have already demonstrated, plasmon USMNPs enclosed in NAs are efficiently excreted after intravenous injection or intranasal instillation in healthy mice. − The hydrodynamic diameter and zeta potential of NAs were measured using Dynamic Light Scattering (DLS), whereas the size was assessed through Transmission Electron Microscopy (TEM) imaging (Figure b,c), and the metal content was determined using Inductively Coupled Plasma–Mass Spectrometry (ICP–MS) (Table ).…”