Pro-inflammatory cytokines associated with clinical severity of dry eye disease of patients with depression

Mrugacz, Małgorzata; Ostrowska, Lucyna; Bryl, Anna; Szulc, Agata; Żelazowska-Rutkowska, Beata; Mrugacz, Grzegorz

doi:10.1016/j.advms.2017.03.003

Cited by 61 publications

(52 citation statements)

References 39 publications

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“…However, when we compared the cytokine levels measured in our samples to the results reported by Mrugacz et.al. examining physiological tear with ELISA method [28], the levels of IL-6, TNFa and IL-17 were much higher in our samples. A similar phenomenon was observed when we compared our data to those obtained by Gupta et al [29] examining eye-drop naive patients with newly diagnosed POAG compared to controls using a different sample collection procedure and a different method than ours.…”

Section: The Analysis Of Tearmentioning

confidence: 52%

Comparative analysis of cytokine profiles of glaucomatous tears and aqueous humour reveals potential biomarkers for trabeculectomy complications

et al. 2019

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Glaucoma is a multifactorial neurodegenerative disease that causes impaired vision and, in advanced cases, blindness. The increasing prevalence of glaucoma due to an ageing population has necessitated the identification of suitable biomarkers for the early detection of the disease. Aqueous humour ( AH ) has been proposed as a source of biomarkers, but it can only be collected using a minor, yet invasive surgical intervention. Tears, however, are constantly available and can be collected any time via noninvasive methods. In order to examine the utility of tear as a surrogate for aqueous humour in biomarker development, we compared the levels of 27 cytokines and chemokines in paired samples of tear and aqueous humour using a Luminex multiplex immunobead‐based technique. Significantly higher levels of cytokines in tear compared to aqueous humour were detected suggesting that tear and aqueous humour are not identical in terms of inflammation response. Furthermore, the levels of IFN ‐γ, GM ‐ CSF and IL ‐5 in tear were significantly lower in patients who developed complications after one year, but no statistically significant changes in cytokine levels were observed in aqueous humour. These three molecules may have potential as predictive biomarkers for the appearance of late flap‐related complications of trabeculectomy.

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Section: The Analysis Of Tearmentioning

confidence: 52%

Comparative analysis of cytokine profiles of glaucomatous tears and aqueous humour reveals potential biomarkers for trabeculectomy complications

et al. 2019

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“…Although the etiology of DED varies and the pathological mechanism is complicated, a common consensus is that the involvement of inflammation as an integral part of DED. Abundant evidences from animal and clinical studies has shown that upregulated of inflammatory cytokines in tears and conjunctival epithelium can result in more severe forms of DED [ 6 , 13 , 18 , 19 ]. In light of the important role in the process of inflammation, there is growing interests on the identification of cytokines that are specifically involved in the pathogenesis of DED.…”

Section: Discussionmentioning

confidence: 99%

Tear Luminex Analysis in Dry Eye Patients

Zhao

et al. 2018

Med Sci Monit

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BackgroundThe purpose of this study was to analyze tear inflammatory cytokines of different subclasses of dry eye disease (DED) patients using Luminex technology.Material/MethodsForty-five DED patients including 20 Sjogren syndrome aqueous tear deficiency (SS-ATD) patients, 20 non-Sjogren syndrome aqueous tear deficiency (NSS-ATD) patients, 15 meibomian gland dysfunction (MGD) patients, and 15 normal participants were enrolled in this study. Concentrations of 11 inflammatory cytokines in tear samples of study participants were measured by Luminex assay; ELISA assay was further applied for validation.ResultsThe levels of cytokines were mostly increased (TNF-α, IL-1α, IL-1β, IL-6, IL-8, IL-12P70, IL-13, IFN-γ, and MIP-1α) in DED patients compared with normal participants. And the levels of TNF-α, IL-6, IL-8, and IL-12P70 were significantly elevated in tears of the patient groups compared to tears of participants in the normal group (P<0.05). Statistical differences were also observed among the patient groups (SS-ATD, NSS-ATD, and MGD) for the level of IL-8 and TNF-α. The results of ELISA assay demonstrated the consistence with Luminex assay, confirming the practicality of Luminex technology for the analysis of multiple cytokines in DED patient tears.ConclusionsThe levels of inflammatory cytokines were mostly elevated in DED patients, and statistical differences of some cytokines were also found between SS-ATD, NSS-ATD, and MGD groups, suggesting that inflammatory cytokines could be potential supplements for the diagnosis of DED subclasses and therapeutic targets for DED patients.

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“…TNFα is one of the main inflammatory cytokines produced by the corneal epithelium in response to hyperosmolarity. 46 In addition, the clinical severity of DED has been correlated with TNFα levels, 55 and TNFα has been found to be elevated in tears from DED patients 56 , 57 and in tear-washings of dry eye mice. 58 These results demonstrate that HMGB1 production is enhanced by a dry eye-like environment.…”

Section: Discussionmentioning

confidence: 99%

High-Mobility Group Box 1 in Dry Eye Inflammation

Lema

Reins

Redfern

2018

Invest. Ophthalmol. Vis. Sci.

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PurposeTo determine high-mobility group box 1 (HMGB1) expression during experimental dry eye (EDE) and dry eye-like culture conditions and elucidate its role in corneal dry eye-related inflammation.MethodsEDE was induced in 8- to 12-week-old C57BL/6 mice. Corneal tissue sections and lysates from EDE and untreated mice were evaluated for HMGB1 expression by immunostaining and quantitative real-time PCR (qPCR). For in vitro studies, human corneal epithelial cells (HCEC) were treated with hyperosmolar media, toll-like receptor (TLR) agonists, or proinflammatory cytokines to determine HMGB1 expression. HCEC were also treated with human recombinant HMGB1 (hrHMGB1) alone or in combination with inflammatory stimuli, and TNFα, IL-6, and IL-8 expression evaluated by qPCR and ELISA. Nuclear factor-κB (NF-κB) p65 nuclear translocation was determined by immunostaining.ResultsEDE mice had higher corneal HMGB1 RNA and protein expression compared to untreated animals. In HCEC, hyperosmolar stress and TNFα treatment stimulated HMGB1 production and secretion into culture supernatants. However, in vitro stimulation with hrHMGB1 did not induce secretion of TNFα, IL-6, or IL-8 or NF-κB p65 nuclear translocation. In addition, the inflammatory response elicited by TLR agonists fibroblast-stimulating lipopeptide-1 and lipopolysaccharide was not enhanced by hrHMGB1 treatment.ConclusionsHMGB1 expression was enhanced by dry eye conditions in vivo as well as in vitro, during hyperosmolar stress and cytokine exposure, suggesting an important role for HMGB1 in dry eye disease. However, no direct inflammatory effect was observed with HMGB1 treatment. Therefore, under these conditions, HMGB1 does not contribute directly to dry eye-induced inflammation and its function at the ocular surface needs to be explored further.

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Pro-inflammatory cytokines associated with clinical severity of dry eye disease of patients with depression

Cited by 61 publications

References 39 publications

Comparative analysis of cytokine profiles of glaucomatous tears and aqueous humour reveals potential biomarkers for trabeculectomy complications

Comparative analysis of cytokine profiles of glaucomatous tears and aqueous humour reveals potential biomarkers for trabeculectomy complications

Tear Luminex Analysis in Dry Eye Patients

High-Mobility Group Box 1 in Dry Eye Inflammation

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