Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa

Regoli, Francesco; Gorbi, Stefania; Machella, Nicola; Tedesco, Sara; Benedetti, Maura; Bocchetti, Raffaella; Notti, Alessandra; Fattorini, Daniele; Piva, Francesco; Principato, Giovanni

doi:10.1016/j.freeradbiomed.2005.08.004

Cited by 75 publications

(45 citation statements)

References 45 publications

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“…Under our experimental conditions it was also possible to note a significant increase of ROS production in two tested cell lines even with a different qualitative and quantitative timecourse. It should be mentioned that "in vivo" experiments in literature also seem to confirm that chronic exposure to ELF-EMF is able to generate a stress oxidative status [32][33][34]. The "in vitro" experiments reported here, as well as the results derived from "in vivo" techniques [13,22,24,25,28,35,36] in mice, show that the presence of ELF-EMF can induce a variable and specie-specific alteration of the stress oxidative pathway.…”

Section: Discussionsupporting

confidence: 78%

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Farina

Mariggiò³

et al. 2010

PIER B

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Abstract-The aim of this paper is to discuss the effects of the exposure to Extremely Low Frequency ElectroMagnetic Fields (ELFEMFs) on non-and excitable cells using in vitro cell models, namely neuron-like cell line (PC12), glioblastoma-derived GL15 cells as glial model and C2C12 myocytes as muscle model, focusing our attention on standardized protocols for ELF-EMFs generation and exposure. A major issue in laboratory -and likely in nature -studies about possible biological effects of ELF waves is the difficulty in providing standard, reproducible environmental conditions. Hence, as part of the work we have developed an exposure system including a probing scanner, able to sample a given volume and to measure the timevarying magnetic field vector. The system allows detection, monitoring and removal of electromagnetic noise sources, as well as means to assess field homogeneity in terms of intensity and polarization.

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Section: Discussionsupporting

confidence: 78%

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Farina

Mariggiò³

et al. 2010

PIER B

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“…In accordance with this hypothesis, redox-related cellular changes following ELF-MFs exposure were reported in the last few years [Regoli et al, 2005;Wolf et al, 2005;Yokus et al, 2005;Zwirska-Korczala et al, 2005;Falone et al, 2007Falone et al, , 2008Hashish et al, 2008;Di Loreto et al, 2009].…”

mentioning

confidence: 54%

Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells

et al. 2011

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Extremely low-frequency magnetic fields (ELF-MFs) may affect human health because of the possible associations with leukemia but also with cancer, cardiovascular, and neurological disorders. In the present work, human SH-SY5Y neuroblastoma cells were exposed to a 50 Hz, 1 mT sinusoidal ELF-MF at three different times, that is, 5 days (T5), 10 days (T10), and 15 days (T15) and then the effects of ELF-MF on proteome expression and biological behavior were investigated. Through comparative analysis between treated and control samples, we analyzed the proteome changes induced by ELF-MF exposure. Nine new proteins resolved in sample after a 15-day treatment were involved in a cellular defense mechanism and/or in cellular organization and proliferation such as peroxiredoxin isoenzymes (2, 3, and 6), 3-mercaptopyruvate sulfurtransferase, actin cytoplasmatic 2, t-complex protein subunit beta, ropporin-1A, and profilin-2 and spindlin-1. Our results indicated that ELF-MFs exposure altered the proliferative status and other important cell biology-related parameters, such as cell growth pattern, and cytoskeletal organization. These findings support our hypothesis that ELF radiation could trigger a shift toward a more invasive phenotype.

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“…Also, under our experimental conditions, it was possible to note a significant modification in ROS production in undifferentiated PC12 cells while the differentiated phenotype seemed not to be affected. Moreover, in vivo experiments also appear to confirm that chronic exposure to ELF-EMFs can generate an oxidative stress status [31,[52][53][54][55][56][57][58][59][60][61]. In addition, the presence of a "stress" status is also demonstrated by the fact that the enzymatic activity of catalase, involved in the catalytic removal of ROS accumulated as a possible effect of ELF-EMF exposure, was decreased during chronic exposure.…”

Section: Discussionmentioning

confidence: 84%

“…a remarkable amount of data support another interesting theory that find in chemical reactions involving free radical production, the target of ELF-EMFs [29]. In fact in the last years, some data from literature have described redox-related cellular changes, following exposure to ELF-EMFs [10,27,[30][31][32].…”

Section: Introductionmentioning

confidence: 83%

Effects of Acute and Chronic Low Frequency Electromagnetic Field Exposure on PC12 Cells during Neuronal Differentiation

Morabito

Guarnieri

Fanò³

et al. 2010

Cell Physiol Biochem

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Background/Aims. The purpose of this study was to provide information about the in vitro neuritogenesis during cell exposure to extremely low frequency electromagnetic fields (ELF-EMFs) of different intensities and durations using pheochromocytoma-derived cell line (PC12 cells) as neuronal model. Methods. Proliferative rates and neuritogenesis were tested by colorimetric assay and morphological analysis, respectively; reactive oxygen species (ROS) levels and intracellular Ca²⁺ variations monitored using single cell videomicroscopy. Results. The long-lasting ELF-EMF exposure (0.1-1.0 mT) did not appear to significantly affect the biological response (proliferation and neuritogenesis). However, during the acute ELF-EMF exposure (30 min), in undifferentiated PC12 cells, there were increased ROS levels and decreased catalase activity, that, conversely, resulted increased after chronic exposure (7 days) at 1.0 mT. Acute exposure (0.1-1.0 mT) affected the spontaneous intracellular Ca²⁺ variations in undifferentiated cells, in which basal intracellular Ca²⁺ resulted increased after chronic exposure. In addition acute exposure affected cell response to a depolarizing agent, while basal membrane potential was not changed. Conclusion. Even if further studies remain necessary to identify the ROS/intracellular Ca²⁺cross-talking pathway activated by ELF-EMF exposure, we support the hypothesis that ROS and Ca²⁺ could be the cellular “primum movens” of the ELF-EMF induced effects on biological systems.

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Pro-oxidant effects of extremely low frequency electromagnetic fields in the land snail Helix aspersa

Cited by 75 publications

References 45 publications

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

ELF-EMFs INDUCED EFFECTS ON CELL LINES: CONTROLLING ELF GENERATION IN LABORATORY

Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells

Effects of Acute and Chronic Low Frequency Electromagnetic Field Exposure on PC12 Cells during Neuronal Differentiation

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