Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Bermúdez, Diego; Escalier, Denise; Gallo, Jean‐Marc; Viellefond, Annick; Ríus, Francisca; Vargas, I. Pérez de; Schrével, Joseph

doi:10.1530/jrf.0.1000567

Cited by 19 publications

(17 citation statements)

References 5 publications

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“…ACR is a specific marker of meiotic and post‐meiotic GCs, and expression of this marker in the culture shows the cell's entry into meiosis (Bermudez et al. ; Marques‐Mari et al. ).…”

Section: Discussionmentioning

confidence: 99%

Induction of Ram Bone Marrow Mesenchymal Stem Cells into Germ Cell Lineage using Transforming Growth Factor‐β Superfamily Growth Factors

Ghasemzadeh-Hasankolaei

Sedighi-Gilani

Eslaminejad

2014

Reprod Domestic Animals

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Several studies have proposed that in vitro generation of germ cells (GCs) from stem cells can be considered a future option for infertility treatment. Mesenchymal stem cells (MSCs) have the capability to differentiate into male GCs with the use of inducers such as retinoic acid. Transforming growth factor-beta 1 (TGFb1) has been shown to play important roles in male fertility and spermatogenesis. Bone morphogenic protein 4 (BMP4) and BMP8b are also involved in the derivation of primordial GCs (PGCs) from epiblast cells. Therefore, this study aims to determine whether TGFb1, BMP4 and BMP8b can initiate transdifferentiation of MSCs into GCs in vitro and to determine the type of changes that occur in the expression of GC-specific markers. In this study, we have divided passage-3 ram bone marrow (BM)-MSCs into three main groups (BMP4, BMP8b and TGFb1) which were separately treated with 10 ng/ml TGFb1, 100 ng/ml BMP4 and 100 ng/ml BMP8b for a period of 21 days. We have evaluated the ability of these groups to differentiate into GCs by assessing expressions of GC-specific markers with reverse transcription PCR (RT-PCR), quantitative RT-PCR (qRT-PCR), immunocytochemistry, morphological changes and alkaline phosphatase (ALP) activity. Our results showed that BMP4 and BMP8b induced PGCs properties in some BM-MSCs and TGFb1 formed spermatogonial stem cells (SSCs) and spermatogonia-like cells in BM-MSCs culture. The important results of this study provide the basis for additional studies to determine the exact mechanism of GCs differentiation and possibly solve the problem of infertility.

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“…ACR is a specific marker of meiotic and post‐meiotic GCs, and expression of this marker in the culture shows the cell's entry into meiosis (Bermudez et al. ; Marques‐Mari et al. ).…”

Section: Discussionmentioning

confidence: 99%

Induction of Ram Bone Marrow Mesenchymal Stem Cells into Germ Cell Lineage using Transforming Growth Factor‐β Superfamily Growth Factors

Ghasemzadeh-Hasankolaei

Sedighi-Gilani

Eslaminejad

2014

Reprod Domestic Animals

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“…[26] Recently, fine needle aspiration (FNA) has been introduced as a simple, lowcost and lowrisk procedure that is adequate for differentiating between obstructive and non-obstructive azoospermia and for assessing different spermatogenic defects as it shows good agreement with open biopsy data. [1027] FNA is also used for sperm retrieval with minimal cost and morbidity.…”

Section: Discussionmentioning

confidence: 99%

Histopathological patterns of testicular biopsy in male infertility: A retrospective study from a tertiary care center in the western part of Saudi Arabia

Abdullah¹,

Bondagji²

2011

Urol Ann

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Objective:To identify and categorize various pathological changes seen in testicular biopsies of males with infertility and to compare the results with data from other local and international studies.Materials and Methods:All testicular biopsies from males with infertility received by the Pathology Department of King AbdulAziz University Hospital, Jeddah, in the period from January 2004 until May 2010 are reviewed and histopathologically classified into seven categories as follows : Normal spermatogenesis, hypospermatogenesis, germ cell maturation arrest (GCMA), Sertoli cell only syndrome, seminiferous tubule hyalinization, mixed and discordant patterns.Results:One hundred testicular biopsies were identified in the computerized records of the Department of Pathology of King AbdulAziz University Hospital in the studied period. The age ranged from 22 to 70 years with a mean age of 24.5 years. The histopathological patterns were as follows: 14 (14%) cases were reported as normal spermatogenesis;(29, 29%) cases as hypospermatogesis; and 12 (12%) cases were reported as GCMA, mostly at the level of primary spermatocytes. The Sertoli cell only syndrome and the seminiferous tubule hyalinization categories were each reported in 16 cases (16%). Nine cases (9%) showed a mixed pattern. Discordant pattern was seen in 5 (5%) cases.Conclusion:Our study showed that hypospermatogenesis is the commonest pattern in testicular biopsies taken from males with infertility in our region. This study supports the recommendation of bilateral testicular biopsies when investigating male infertility.

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“…According to the acrosome formation, spermiogenesis can be divided into several steps, which in turn can be grouped into four phases: Golgi, Cap, Acrosome, and Maturation (Table 1). It is normally accepted that the acrosome formation starts with spermiogenesis, but in humans, rhesus monkeys, and mice, the synthesis of many acrosome-specific proteins, such as proacrosin and acrogranin, begins at the pachytene spermatocyte step (Bermudez et al, 1994;Moreno et al, 2000a). The biosynthetic routes taken by species such as cattle, pigs, and rats are different because the proacrosin gene is expressed in the round spermatid and not in the spermatocytes (Kremling et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

The mammalian acrosome as a secretory lysosome: New and old evidence

Moreno

Alvarado

2006

Molecular Reproduction Devel

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The morphological and biochemical characteristics of the acrosome depart well from any other vesicles in somatic cells, making it one of a kind amongst secretory vesicles. The components of the acrosome include a mixture of unique enzymes like acrosin and other enzymes that when present in somatic cells are commonly found in lysosomes, peroxisomes, and even in the cytoplasm. Several observations have pointed out that acrosomal biogenesis has unique features not previously described in secretory vesicle biogenesis of somatic cells. In this review we discuss the evidence supporting a molecular link between the machinery involved in lysosome and acrosome biogenesis, link which may help account for the acrosome unique composition.

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Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Abstract: A quantitative immunohistochemical study of human spermatogenesis was performed using the 4D4 anti-proacrosin monoclonal antibody (mAb 4D4)

Cited by 19 publications

References 5 publications

Induction of Ram Bone Marrow Mesenchymal Stem Cells into Germ Cell Lineage using Transforming Growth Factor‐β Superfamily Growth Factors

Induction of Ram Bone Marrow Mesenchymal Stem Cells into Germ Cell Lineage using Transforming Growth Factor‐β Superfamily Growth Factors

Histopathological patterns of testicular biopsy in male infertility: A retrospective study from a tertiary care center in the western part of Saudi Arabia

The mammalian acrosome as a secretory lysosome: New and old evidence

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