Probenchemie <sup>15</sup>N–markierter Stickstoffverbindungen im Mikro– bis Nanomolbereich für die emissionsspektrometrische Isotopenanalyse

Faust, H.

doi:10.1080/10256016708622391

Cited by 76 publications

(16 citation statements)

References 2 publications

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“…The NH 4 CI solution obtained was taken directly for ' S N-analysis with an Isonitromat emission spectrometer according to the procedure described by Faust (1967).…”

Section: Methodsmentioning

confidence: 99%

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

Souffrant

Rérat²,

Jp³

et al. 1993

Reprod. Nutr. Dev.

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Summary ― The aim of the present study was to measure the incorporation of infused 15 N in blood fractions, urine, digesta, faeces and in the exocrine pancreatic and biliary secretions, in order to estimate the endogenous part of nitrogen in the ileal digesta and in the faeces of pigs fed a casein diet and to calculate the total endogenous nitrogen secretion as well as its recycling in the digestive tract.

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“…The NH 4 CI solution obtained was taken directly for ' S N-analysis with an Isonitromat emission spectrometer according to the procedure described by Faust (1967).…”

Section: Methodsmentioning

confidence: 99%

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

Souffrant

Rérat²,

Jp³

et al. 1993

Reprod. Nutr. Dev.

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“…Duplicate analyses of '''N enrichments were carried out on each of the preparations of the various N fractions according to the sodium hypobromite method of Faust (1967). A Statron NOI 4 atomic emission spectrophotometer (Packard Instruments, S.A., Zurich, Switzerland) was used for the '"'N assays.…”

Section: "•W Analysismentioning

confidence: 99%

AN INVESTIGATION INTO NITROGEN ASSIMILATION AND DISTRIBUTION IN FRUITING PLANTS OF BARLEY (HORDEUM VULGARE L. cv. CLIPPER) IN RESPONSE TO NITRATE, AMMONIUM AND MIXED NITRATE AND AMMONIUM NUTRITION

Soares

Lewis

1986

New Phytologist

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SUMMARYBarley plants {Hordetim vulgare L. cv. Clipper) were grown to maturity in pH-controlled, aerated solution culture with 2 mM inorganic '"'N supplied as nitrate alone, ammonium alone or 1 :1 nitrate + ammonium. At the end of the eighth week of growth, when seed filling was well advanced, the "N feeding solutions were replaced by "'N feeding solutions, and the plants were grown in these media for 6 and 12 h before harvesting. "'N analyses of the plant material show that even at this late developmental stage, barley plants are still accumulating and assimilating considerable quantities of nitrogen (N), although at a much slower rate than in younger plants. Much of this N is incorporated into leaf protein. Transport of newly assimilated N from vegetative to reproductive parts is rapid, 22 "o of the assimilated (organic) '''N being found in the seed of NOj^-fed plants at harvest, 26 "" in NH/-fed plants and 17 % in NH/-fed plants. Inorganic N sources are not translocated to the seed In significant quantities. NH/ and NH4+ + NO.,"-fed plants show a much more rapid incorporation of N into both vegetative and reproductive organs than do NO,,^-fed plants; seeds of NH^^ + NO^^-fed plants gained organic '•'N at a rate of 8-6 /fg h~' per plant, NH.,+-fed plants at 5-5 //g h"' per plant and NO.,--fed plants at 2-1 jig h"' per plant. The poor organic ' •'N accumulation observed in NO^^-i'ed plants is ascribed to the slow absorption rate of NO.," ions relative to NH,,+ ions and the low nitrate reducing potential of the vegetative organs of the plant at this stage of development.

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“…The 15N label of the amino acids was determined by emission-spectroscopy. N2 was developed essentially as described in Faust (9).…”

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confidence: 99%

Aminotransfer from Alanine and Glutamate to Glycine and Serine during Photorespiration in Oat Leaves

Betsche

1983

Plant Physiol.

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ABSTRACT"N-Labeled glutamate and alanine were used to examine the photorespiratory nitrogen metabolism in oat (A vena sativa L.) leaf slices. Glutamate and alanine supply amino groups for glycine formation during photorespiration. The nitrogen flux from alanine to glycine was estimated to be 3 times higher than that from glutamate. It is concluded from these results that alanine is a direct and important amino donor for photorespiratory glycine formation in oat leaves. The 'N labeling of serine was almost as high as that of glycine during the initial period of the labeling experiments. Thereafter, the ratio of "N label in serine to "N label in glycine declined substantially.During photorespiration glycine is formed from glyoxylate. This process occurs in the leaf peroxisomes. The glycine enters the mitochondria, where two glycine are converted into one serine (24). In this complex reaction, CO2 and NH3 are liberated. The serine reenters the leaf peroxisomes, where serine:glyoxylate aminotransferase mediates the formation of hydroxypyruvate and glycine. By this process, one amino-nitrogen is directly recycled for glycine formation. The nitrogen of the other amino group, liberated as NH3, is refixed to glutamate via the glutamine synthetase/glutamate synthase pathway (1 1, 13, 22, 30). The finding that leaf peroxisomes contain glutamate:glyoxylate aminotransferase leads to the suggestion that the additional amino nitrogen needed for the formation of the second glycine is provided by this glutamate (13). However, there are a number of reports indicating that leaf extracts, or leaf peroxisomes, have substantial alanine:glyoxylate aminotransferase activity that may even exceed the glutamate:glyoxylate aminotransferase activity (4,7,21,24,25,28). Alanine is the main substrate for the formation of glycine also in the microbodies (peroxisomes) of the alga Mougeotia (29). In higher plants, most of the alanine:glyoxylate aminotransferase activity is attributed to the glutamate:glyoxylate aminotransferase (21,24,25). Glutamate:glyoxylate aminotransferase is also responsible for the glutamate:pyruvate and the alanine:a-ketoglutarate aminotransferase activities of leaf peroxisomes (3, 12,19,20 Preparation of Leaf Slices. The leaves were cut into slices (1.5-2.0 mm) with a razor blade. Four g of the leaf slices were vacuuminfiltrated in 40 ml of an osmotic medium consisting of 0.3 M sorbitol, 2 nim KH2PO4, 2 mM MgCl2, 1 mim MnCl2, 1 mm EDTA, and 5 mm Hepes/KOH, pH 7.9. Care was taken to release the vacuum slowly. Before use, the leaf slices were rinsed in a sieve with 40 ml of the same medium.Labeling Experiments. The leaf slices were transferred into a cuvette (8 x 5 x 2 cm) filled with 55 ml of the osmotic medium described above. The latter was bubbled with air for 3 h before being used. The leaf slices were stirred gently from the top, and the cuvette was illuminated with slide projectors from both sides the leaf slices were quickly transferred into a sieve, rinsed with 30 ml of water, and dipped several times into a large...

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Probenchemie ¹⁵N–markierter Stickstoffverbindungen im Mikro– bis Nanomolbereich für die emissionsspektrometrische Isotopenanalyse

Cited by 76 publications

References 2 publications

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

AN INVESTIGATION INTO NITROGEN ASSIMILATION AND DISTRIBUTION IN FRUITING PLANTS OF BARLEY (HORDEUM VULGARE L. cv. CLIPPER) IN RESPONSE TO NITRATE, AMMONIUM AND MIXED NITRATE AND AMMONIUM NUTRITION

Aminotransfer from Alanine and Glutamate to Glycine and Serine during Photorespiration in Oat Leaves

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Probenchemie 15N–markierter Stickstoffverbindungen im Mikro– bis Nanomolbereich für die emissionsspektrometrische Isotopenanalyse

Cited by 76 publications

References 2 publications

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

Exogenous and endogenous contributions to nitrogen fluxes in the digestive tract of pigs fed a casein diet. III. Recycling of endogenous nitrogen

AN INVESTIGATION INTO NITROGEN ASSIMILATION AND DISTRIBUTION IN FRUITING PLANTS OF BARLEY (HORDEUM VULGARE L. cv. CLIPPER) IN RESPONSE TO NITRATE, AMMONIUM AND MIXED NITRATE AND AMMONIUM NUTRITION

Aminotransfer from Alanine and Glutamate to Glycine and Serine during Photorespiration in Oat Leaves

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Probenchemie ¹⁵N–markierter Stickstoffverbindungen im Mikro– bis Nanomolbereich für die emissionsspektrometrische Isotopenanalyse