Probing bacterial transmembrane histidine kinase receptor–ligand interactions with natural and synthetic molecules

Ng, Wai Leung; Wei, Yunzhou; Perez, Lark J.; Cong, Jian-Ping; Long, Tao; Koch, Matthew J.; Semmelhack, M. F.; Wingreen, Ned S.; Bassler, Bonnie L.

doi:10.1073/pnas.1001392107

Cited by 58 publications

(138 citation statements)

References 53 publications

(58 reference statements)

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“…The synthetic autoinducers were produced as described previously (25)(26)(27)(28) and were provided as a kind gift by Bonnie L. Bassler and Wai-Leung Ng, (Princeton University, Princeton, NJ and Tufts University, Medford, MA, respectively). The CAI-1 preparation was dissolved in dimethyl sulfoxide to prepare a 5-mM stock solution.…”

Section: Methodsmentioning

confidence: 99%

Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples

Bari

Roky

Mohiuddin

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Cholera epidemics have long been known to spread through water contaminated with human fecal material containing the toxigenic bacterium Vibrio cholerae. However, detection of V. cholerae in water is complicated by the existence of a dormant state in which the organism remains viable, but resists cultivation on routine bacteriological media. Growth in the mammalian intestine has been reported to trigger "resuscitation" of such dormant cells, and these studies have prompted the search for resuscitation factors. Although some positive reports have emerged from these investigations, the precise molecular signals that activate dormant V. cholerae have remained elusive. Quorum-sensing autoinducers are small molecules that ordinarily regulate bacterial gene expression in response to cell density or interspecies bacterial interactions. We have found that isolation of pathogenic clones of V. cholerae from surface waters in Bangladesh is dramatically improved by using enrichment media containing autoinducers either expressed from cloned synthase genes or prepared by chemical synthesis. These results may contribute to averting future disasters by providing a strategy for early detection of V. cholerae in surface waters that have been contaminated with the stools of cholera patients or asymptomatic infected human carriers.biofilm formation | CVEC | transmissibility T he natural habitats of the species Vibrio cholerae are estuarine or fresh water aquatic environments (1-3). In cholera endemic areas such as Bangladesh, viable V. cholerae can be readily detected in water during seasonal cholera epidemics; however, as disease incidence decreases, the isolation of viable V. cholerae becomes dramatically more difficult perhaps in part due to the influence of lytic bacteriophages (4, 5). However, during the interepidemic period, the organism can also occasionally be found in a viable but dormant state, which has been alternately referred to as the viable but nonculturable (VBNC) cells (1), conditionally viable environmental cells (CVEC) (6), or active but nonculturable (ABNC) (7). Recently we have documented the existence of CVEC in surface waters of Bangladesh by using fluorescent antibody based microscopy, which revealed clumps of V. cholerae O1 cells in water, which were often negative for V. cholerae O1 by conventional culture. To detect possible presence of relatively small number of culturable cells in such water we also used enrichment and selection approaches that depend on antibiotic resistance profiles displayed by the strains that have caused the preceding cholera epidemic. This approach referred to as antibiotic selection technique (AST) (8) allowed enhanced detection of V. cholerae by suppressing growth of other environmental bacteria that would otherwise mask the small number of V. cholerae colonies.In our previous studies, CVEC were found to be organized as aggregates of cells embedded in extracellular material, presumably Vibrio extracellular polysaccharide (VPS) (6). The genes responsible for VPS production are c...

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Section: Methodsmentioning

confidence: 99%

Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples

Bari

Roky

Mohiuddin

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…1, a and b, rightmost bars). We showed previously that introduction of cqsA into recombinant E. coli is sufficient for high level production of CAI-1 (7,33). In all cases C. elegans preferred bacteria that produce CAI-1 to those that do not, and C. elegans preferred fluids collected from bacterial cultures containing CAI-1 compared with those lacking CAI-1.…”

Section: Resultsmentioning

confidence: 99%

“…Specifically, we found the order of chemotactic preference to be: CAI-1 ϭ (C9)-CAI-1 Ͼ (C8)-CAI-1 ϭ (C11)-CAI-1. Interestingly, this order of preference for tail length exactly parallels the CqsS receptor preference (9,28,33). To assess the generality of this response to other bacterial signaling molecules containing fatty acid tails, we examined the responses of C. elegans to a series of acylated homoserine lactone AIs.…”

Section: Resultsmentioning

confidence: 99%

Caenorhabditis elegans Recognizes a Bacterial Quorum-sensing Signal Molecule through the AWCON Neuron

Werner

Perez

Ghosh

et al. 2014

Journal of Biological Chemistry

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Background:The nematode Caenorhabditis elegans consumes bacteria as its sole food source. Results: The bacterium Vibrio cholerae produces a quorum-sensing signal molecule called CAI-1, which C. elegans detects through the AWC ON chemosensory neuron. Conclusion: C. elegans uses bacterial-produced molecules as cues, and these molecules are also physiologically significant to bacteria. Significance: The V. cholerae molecule CAI-1 enables cross-kingdom chemical interaction.

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“…1). LuxO is the point of convergence of both the system I and II [2,31,34,35]. System III converges with System I and System II at LuxO protein through activation of VarS/A pathway but the exact mechanism of the series of steps that occur to activate LuxO is unknown [2,32].…”

Section: Quorum Sensing In Vibrio Choleraementioning

confidence: 99%

Meddling Vibrio cholerae Murmurs: A Neoteric Advancement in Cholera Research

2015

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Cholera, a known diarrheal disease is associated with various risk factors like hypovolemic shock, rice watery stools, and death in developing countries. The overuse of antibiotics to treat cholera imposed a selective pressure for the emergence and spread of multi-drug resistant Vibrio cholerae strains. The failure of conventional antimicrobial therapy urged the researchers to find an alternative therapy that could meddle the cholera murmurs (Quorum Sensing). It seems to effectively overcome the conventional cholera therapies in parallel to decrease the morbidity and mortality rate in the developing countries. The paramount objective of this review essentially focuses on the different Quorum Sensing (QS) regulatory switches governing virulence and pathogenicity of Vibrio cholerae. This review also provides an insight into the plausible QS targets that could be exploited to bring about a breakthrough to the prevailing cholera therapy.

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Probing bacterial transmembrane histidine kinase receptor–ligand interactions with natural and synthetic molecules

Cited by 58 publications

References 53 publications

Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples

Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples

Caenorhabditis elegans Recognizes a Bacterial Quorum-sensing Signal Molecule through the AWCON Neuron

Meddling Vibrio cholerae Murmurs: A Neoteric Advancement in Cholera Research

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