Probing biomolecular interaction forces using an anharmonic acoustic technique for selective detection of bacterial spores

Ghosh, Sourav; Ostanin, Victor P.; Johnson, Christian L.; Lowe, Christopher R.; Seshia, Ashwin A.

doi:10.1016/j.bios.2011.08.008

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…Although the degree of non-complementarity of the sequences was not investigated as part of this work, and it is possible that the random sequence had some degree of affinity towards the receptor sequence, the specificity using the third harmonic signal is comparatively much better. Improved specificity in transduction using third harmonic shift signal agrees with that observed in our previous work [7,8,22].…”

Section: Selectivity Of Detectionsupporting

confidence: 90%

Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator

Swarbrick

Efimov

Ostanin

et al. 2018

Electrochimica Acta

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The paper describes a sensor for single-stranded DNA (ssDNA) biomarker based on anharmonic acoustic signals arising during hybridization with complementary thiolated ssDNA functionalised on the gold electrode of an electrochemical quartz crystal resonator. The steps of sensor preparation and hybridization are carried out in an electrochemical microfluidic flowcell. While the electrochemical impedance spectroscopy does not allow a definitive interpretation, the changes in resonance frequency and third Fourier harmonic current of the resonator on actuation at the fundamental mode indicate formation of a flexibly bound layer. The functionalization and hybridization steps monitored by the anharmonic detection technique (ADT) are described with a simple model based on Duffing nonlinear equation.

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Section: Selectivity Of Detectionsupporting

confidence: 90%

Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator

Swarbrick

Efimov

Ostanin

et al. 2018

Electrochimica Acta

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“…When relatively large (d>1µm) and more flexible adsorbates bind to the quartz crystal via small contact points, they form a coupled oscillator with the quartz crystal resonator. The binding of colloidal particles 7 and biological particles, such as bacterial spores 8 , to the quartz crystal resonator via ligand-receptor bonds are examples of such coupled resonators. The mass coupling is weak for these particles.…”

Section: Introductionmentioning

confidence: 99%

“…In our earlier work, the quartz crystal was driven close to its fundamental resonance mode and the electrical current was measured exactly at three times the drive frequency (3f), referred to as the third Fourier harmonic 5,8 . It should be noted that the third Fourier harmonic is a result of nonlinear response of an oscillator and may be present even in an oscillator with a single-mode of resonance, such as a spring-mass oscillatory system.…”

Section: Introductionmentioning

confidence: 99%

“…The quantitative change in the 3f current was proportional to the number of bound microparticles, and interestingly, significantly smaller for physisorbed microparticles, where the particleresonator interaction was weaker. The transduction method based on the measurement of the change in the third Fourier harmonic current was referred to as the Anharmonic Detection Technique (ADT) 5,8 .…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of particle-surface interactions via anharmonic acoustic transduction

Granja

Sandström

Efimov

et al. 2018

Sensors and Actuators B: Chemical

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Most transduction methods for measuring particle-surface interactions are unable to differentiate the strength of interaction and largely reliant on extensive washing to reduce the ubiquitous non-specific background. Label-based methods, in particular, are limited in wide applicability due to their inherent operational complexity. On the other hand, label-free force-spectroscopic methods that can differentiate particlesurface interaction strength are skill-demanding and time-consuming. Here, we present a label-free anharmonic (nonlinear) acoustic transduction method employing the quartz crystal resonator that reads out ligand-receptor binding based on the interaction strength. We show that while stronger specific interactions are transduced more strongly, and in linear proportionality to the ligand concentration on microparticles, non-specific interactions are significantly attenuated. This allows ligand quantification with high specificity and sensitivity in realtime under flow without separate washing steps. Constructing an analytical model of a quartz resonator, we can relate the number and type (specific vs. non-specific) of ligandreceptor interactions with the change in characteristic nonlinearity coefficient of the resonator. The entirely-electronic and microfluidic-integrable transduction method could potentially allow a simple, fast and reliable way for characterising particlesurface interactions with economy of scale.

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“…Reverse-transcription PCR (RT-PCR) is commonly used in the detection of norovirus and there are many assays that have been proposed. Gregory et al (2011) One such study (Tang, Flandre et al, 2011) reported an inter-digitated array microelectrode-oxide- Anharmonic acoustic techniques were utilized to detect bacterial spores (Bacillus subtilis) in a study by Ghosh et al (2011). This sensor used a thickness-shear mode AT-cut quartz crystal and the sensor was subjected to a pure sinusoidal oscillation.…”

Section: Introductionmentioning

confidence: 99%

Detection and Occurrence of Indicator Organisms and Pathogens

Bhaduri¹,

Stedtfeld²,

Srinivasan³

et al. 2012

Water Environment Research

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This review summarizes key studies published in 2011 related to the detection and occurrence of fecal indicators and pathogens. The first section is a summary of the key findings and developments in detection methods for fecal indicators using polymerase chain reaction (PCR), microarrays, label-free detection methods, isothermal amplification methods, immunoassays, microfluidics, and also advances in sample processing. The second section focuses on key studies in microbial source tracking (MST) and includes genotypic and phenotypic methods. The third section deals with studies on the occurrence, persistence and transport of conventional and alternative indicators.

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Probing biomolecular interaction forces using an anharmonic acoustic technique for selective detection of bacterial spores

Cited by 10 publications

References 24 publications

Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator

Anharmonic acoustic effects during DNA hybridization on an electrochemical quartz crystal resonator

Characterisation of particle-surface interactions via anharmonic acoustic transduction

Detection and Occurrence of Indicator Organisms and Pathogens

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