Probing Ligand–Protein Recognition with Sum‐Frequency Generation Spectroscopy: The Avidin–Biocytin Case

Dreesen, Laurent; Sartenaer, Yannick; Humbert, Christophe; Mani, A. A.; Méthivier, Christophe; Pradier, Claire‐Marie; Thiry, P.A.; Péremans, A.

doi:10.1002/cphc.200400213

Cited by 42 publications

(31 citation statements)

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“…AuNps were deposited either on silicon in external reflection configuration [4] or on glass substrates in total internal reflection configuration [5] to compare the sensitivity and molecular ordering of functionalized AuNp films as a function of the surface coverage on glass [6], to unravel the orientation of grafted molecules on AuNps and AgNps [7] and to extract and deduce the nature of the vibration modes thanks to the SPR amplification on functionalized AuNp films in the fingerprint spectral range of aromatic molecules with density functional theory (DFT) calculations [8]. In a general way, SFG has also proven its efficiency in biomolecular recognition on glass substrate [9], in DNAbased biosensors on Pt(111) single crystal [10] and in enlightening DNA hybridization on glass [11] and (100)-facetted gold films [12]. In summary, SFG/DFG spectroscopy is a promising tool in describing the chemistry occurring in biological recognition [13,14] performed on nanostructured biosensors provided that we light on/off the SPR amplification in a controlled manner: AuNps nature, size, shape, and dispersion (surface coverage and lattice parameter) on the substrate, exciting visible wavelength.…”

Section: Introductionmentioning

confidence: 99%

Optical spectroscopy of functionalized gold nanoparticles assemblies as a function of the surface coverage

et al. 2013

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Layers of thiophenol functionalized spherical gold nanoparticles grafted on Si(100) are probed by linear UV-vis, Fourier transform infrared and nonlinear infrared-visible vibrational sum/difference-frequency generation spectroscopies as a function of the nanoparticles surface coverage. Depending on the dipping time (5 min, 20 min, 1 h, and 24 h) in the colloidal solution, AFM imaging corroborates that the silicon surface coverage with gold nanoparticles increases, while the distance between neighbouring nanoparticles decreases, leading to their aggregation which dramatically impacts their optical properties. In the UV-vis reflectance spectra after the appearance of the 525-nm individual plasmonic band, a second broad band located at 660 nm and related to the gold nanoparticles aggregation on silicon rapidly dominates in intensity. Nonlinear vibrational spectroscopy is able to detect the specific vibration of the thiophenol molecules (3, 055 cm −1 ) whatever the immersion time and at least down to 1 % of the substrate filling factor by the gold nanoparticles, overtaking the molecular sensitivity threshold of surface infrared and Raman spectroscopies on small gold nanostructures (17 nm) adsorbed on a semiconductor. Moreover, a quantitative analysis of the nonlinear vibrational fingerprint from 5 min to 24 h in the framework of the effective medium models of Maxwell-Garnett and Bruggeman illustrates the role played by the interband and the plasmonic properties of gold modulated by the silicon optical response. In this case, the sample reflectivity affects the molecular oscillator strength measured by nonlinear optical vibrational spectroscopy. For this latter technique, no coupling with the optical properties of aggregated AuNps is evidenced while the localized surface plasmon resonance excitation amplifies the molecular response.

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Section: Introductionmentioning

confidence: 99%

Optical spectroscopy of functionalized gold nanoparticles assemblies as a function of the surface coverage

et al. 2013

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“…30–43 Recently, SFG has also been employed to study biomolecules such as lipids, peptides and proteins which further progressed this research field. 44–61 This article presents an overview of studies that have been done in our group that sheds light on elucidating molecular level information about interfacial peptides/proteins involved in different biological environments as well as processes.…”

Section: Introductionmentioning

confidence: 99%

Molecular Interactions of Proteins and Peptides at Interfaces Studied by Sum Frequency Generation Vibrational Spectroscopy

2011

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Interfacial peptides and proteins are critical in many biological processes and thus are of interest to various research fields. To study these processes, surface sensitive techniques are required to completely describe different interfacial interactions intrinsic to many complicated processes. Sum frequency generation (SFG) spectroscopy has been developed into a powerful tool to investigate these interactions and mechanisms of a variety of interfacial peptides and proteins. It has been shown that SFG has intrinsic surface sensitivity and the ability to acquire conformation, orientation and ordering information about these systems. This paper reviews recent studies on peptide/protein-substrate interactions, peptide/protein-membrane interactions and protein complexes at interfaces and demonstrates the ability of SFG on unveiling the molecular pictures of complicated interfacial biological processes.

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“…The binding time was fixed at 22 h with stirring, followed by the washing and rinsing steps with HEPES and DI, respectively. Before starting the scanning process, the slides were stored to rest for 30 min in the printer [23].…”

Section: Biotin-strepavidin Reactionmentioning

confidence: 99%

Dual detection biosensor based on porous silicon substrate

Simion

Kusko

Mihalache

et al. 2013

Materials Science and Engineering: B

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Probing Ligand–Protein Recognition with Sum‐Frequency Generation Spectroscopy: The Avidin–Biocytin Case

Cited by 42 publications

References 41 publications

Optical spectroscopy of functionalized gold nanoparticles assemblies as a function of the surface coverage

Optical spectroscopy of functionalized gold nanoparticles assemblies as a function of the surface coverage

Molecular Interactions of Proteins and Peptides at Interfaces Studied by Sum Frequency Generation Vibrational Spectroscopy

Dual detection biosensor based on porous silicon substrate

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