Probing molecules by surface-enhanced Raman spectroscopy

Netti, M.C.; Zoorob, M.E.; Charlton, Martin D. B.; Ayliffe, P.; Mahnkopf, S.; Stopford, Paul; Todd, Kathryn G.; Lincoln, John R.; Perney, N.M.B.; Baumberg, Jeremy J.

doi:10.1117/12.646368

Cited by 14 publications

(23 citation statements)

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“…The process results in an array of highly reproducible inverted pyramid structures. 52 These array pyramids are reported to have "hot spots" or "trapped plasmons" located inside the wells. 52 Additionally, due to the fabrication process used, under ideal conditions these substrates have demonstrated relative standard deviations, RSDs ranging from 10-15%.…”

Section: Klarite Sers Substratesmentioning

confidence: 99%

“…52 Additionally, due to the fabrication process used, under ideal conditions these substrates have demonstrated relative standard deviations, RSDs ranging from 10-15%. 52 While these substrates do demonstrate a high degree of substrate reproducibility and very low substrate background (SERS signal and surface morphology) for applications to real-world situations increased analyte sensitivity is still necessary (low signal-to-noise, S/N ratios). …”

Section: Klarite Sers Substratesmentioning

confidence: 99%

“…This innate biological ability to guide and assemble nanoscale components into precisely controlled and sophisticated structures from very basic components has motivated intense defense, industrial and academic research efforts to develop and harness these directed artificial methods that mimic or exploit the selective recognition capabilities and sensitive interactions inherently found in biological systems. Many of these approaches have resulted in the successful merging of more traditional sensing techniques (Fluorescence 53 , Raman 54 , Raman-based spectroscopic techniques 1,30,52,[55][56][57][58] ) in novel nano-scale synthetic platforms combined with biomolecular recognition capabilities, thus demonstrating viable and novel transformative solutions for today's sensing needs.…”

Section: Peptide-based Sensingmentioning

confidence: 99%

“…[48][49][50][51][52] These substrates were developed using Si-based semiconductor fabrication techniques. 52 Klarite substrates are fabricated using a well-defined Silicon fabrication technique in which a silicon diode mask is defined by optical lithography, and then KOH surface etched. The process results in an array of highly reproducible inverted pyramid structures.…”

Section: Klarite Sers Substratesmentioning

confidence: 99%

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The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

et al. 2016

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The utility of peptide-based molecular sensing for the development of novel biosensors has resulted in a significant increase in their development and usage for sensing targets like chemical, biological, energetic and toxic materials. Using peptides as a molecular recognition element is particularly advantageous because there are several mature peptide synthesis protocols that already exist, peptide structures can be tailored, selected and manipulated to be highly discerning towards desired targets, peptides can be modified to be very stable in a host of environments and stable under many different conditions, and through the development of bifunctionalized peptides can be synthesized to also bind onto desired sensing platforms (various metal materials, glass, etc.). Two examples of the several Army relevant biological targets for peptide-based sensing platforms include Ricin and Abrin. Ricin and Abrin are alarming threats because both can be weaponized and there is no antidote for exposure. Combining the sensitivity of SERS with the selectivity of a bifunctional peptide allows for the emergence of dynamic hazard sensor for Army application.

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Section: Klarite Sers Substratesmentioning

confidence: 99%

Section: Klarite Sers Substratesmentioning

confidence: 99%

Section: Peptide-based Sensingmentioning

confidence: 99%

Section: Klarite Sers Substratesmentioning

confidence: 99%

See 2 more Smart Citations

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

et al. 2016

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“…The recent commercial availability of SERS-substrates produced by semiconductor techniques has overcome the well-known limitation in reproducibility of enhancement on substrate surfaces. 10,11 These substrates are based on silicon photonic crystals, which are etched with void architectures and coated with a layer of gold making a SERS-active surface. In this paper, we explore the feasibility of using these gold-coated SERS substrates for the rapid and reproducible detection of synovial fluid HA at clinically useful levels.…”

Section: Introductionmentioning

confidence: 99%

Detection of potential osteoarthritis biomarkers using surface enhanced Raman spectroscopy in the near-infrared

et al. 2006

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A novel application of surface-enhanced Raman spectroscopy (SERS) for in-vitro osteoarthritis (OA) biomarker detection is described. Hyaluronic acid (HA) is a potential OA biomarker and synovial fluid levels of HA have been correlated with progression of joint space narrowing. However, current immunoassay and chromatographic methods that identify HA in synovial fluid are cumbersome and often require sophisticated instrumentation. Raman spectroscopy may be an alternative to these analytical methods, providing rapid identification of HA using characteristic Raman bands. Yet, previous reports of un-enhanced Raman spectroscopy for hyaluronic acid are in aqueous solutions exceeding 1000X in-vivo concentrations because HA is a weakly scattering polysaccharide. Surface-enhanced Raman spectroscopy can improve detection limits by 100-1000 times and we present, to our best knowledge, the first surface-enhanced Raman spectra of hyaluronic acid. Moreover, the recent commercial availability of stable SERS gold substrates has enabled rapid SERS detection of this biomarker at concentrations diluted by more than an order of magnitude, compared to previous literature reports. Preliminary results of easily and rapidly observing hyaluronic acid at low concentrations in aqueous solutions supported further studies in synthetic models of biofluids, such as artificial synovial fluid, that contain HA at low concentrations. These complex fluids contain proteins that compete for the SERS-active sites on the substrate, and the resulting spectra are dominated by protein Raman bands. We apply a simple and validated protein precipitation protocol to artificial synovial fluid prior to deposition onto the SERS substrate. We find that HA is easily detected in these fluids after protein removal treatment.

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Time Fluctuations and Imaging in the SERS Spectra of Fungal Hypha Grown on Nanostructured Substrates

et al. 2007

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Surface-enhanced Raman scattering (SERS) spectroscopy is an emerging technique in biomolecular analysis that can have a tremendous impact in the life sciences. We report on the SERS imaging of fungal hyphae grown on nanostructured SERS active substrates engineered using semiconductor technologies. Time fluctuations in the intensity and band position in the SERS spectra measured on the same sample position with 1 s integration time have been observed indicating that the SERS signal arises from a limited number of molecules and that possibly single components are being detected.

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Probing molecules by surface-enhanced Raman spectroscopy

Cited by 14 publications

References 0 publications

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

The development of Army relevant peptide-based surface enhanced Raman scattering (SERS) sensors for biological threat detection

Detection of potential osteoarthritis biomarkers using surface enhanced Raman spectroscopy in the near-infrared

Time Fluctuations and Imaging in the SERS Spectra of Fungal Hypha Grown on Nanostructured Substrates

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