Probing protein nanopores with poly(ethylene glycol)s

Liu, Wenxing; Nestorovich, Ekaterina M.

doi:10.1002/pmic.202100055

Cited by 5 publications

(4 citation statements)

References 116 publications

(307 reference statements)

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“…PEG Solutions. Typically, experiments of PEG equilibrium partitioning in protein channels 10 or with PEG as crowding agent or osmotic stress inducer are performed in PEG solutions of varying concentration, although very often in the dilute or semidilute regime. To prove PEG exclusion near a charged membrane in a more realistic environment, we ran 1 μs all-atom MD simulations in 0.1 M KCl solutions containing 10% PEG600 (w/w).…”

Section: Neutron Reflectometry (Nr)mentioning

confidence: 99%

“…PEG is also widely used in many different practical contexts, including the pharmaceutical and food industries, protein precipitation, , and biomedical applications involving drug delivery through PEGylation . PEG is also a versatile biophysical toolbox to induce osmotic stress in polymer-excluded regions , or to probe protein ion channels to get information about their size or their access resistance. , …”

Section: Introductionmentioning

confidence: 99%

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Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure

Aguilella-Arzo,

Hoogerheide,

Doucet

et al. 2024

J. Am. Chem. Soc.

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Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein–protein interactions, and intracellular transport drive a growing interest in quantifying the effects of crowding. While the properties of crowded solutions have been extensively studied, less attention has been paid to the interaction of crowders with the cellular boundaries, i.e., membranes. However, membranes are key components of cells and most subcellular organelles, playing a central role in regulating protein channel and receptor functions by recruiting and binding charged and neutral solutes. While membrane interactions with charged solutes are dominated by electrostatic forces, here we show that significant charge-induced forces also exist between membranes and neutral solutes. Using neutron reflectometry measurements and molecular dynamics simulations of poly(ethylene glycol) (PEG) polymers of different molecular weights near charged and neutral membranes, we demonstrate the roles of surface dielectrophoresis and counterion pressure in repelling PEG from charged membrane surfaces. The resulting depletion zone is expected to have consequences for drug design and delivery, the activity of proteins near membrane surfaces, and the transport of small molecules along the membrane surface.

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Section: Neutron Reflectometry (Nr)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure

Aguilella-Arzo,

Hoogerheide,

Doucet

et al. 2024

J. Am. Chem. Soc.

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“…Nanopore-based protein and peptide sensing reviews Finally, we are pleased to present two brief reviews covering important topics in nanopore sensing with connections to protein and peptide analysis. The first, by Liu and Nestorovich [21], provides a thorough review of the biophysical processes involved in polymer transport in porins and ion channels. They offer a detailed history of the development and use of synthetic polymers (e.g., polyethylene glycol) for probing the geometry of water-filled proteins.…”

Section: 3mentioning

confidence: 99%

Highlights on the current state of proteomic detection and characterization with nanopore sensors

Robertson

Reiner

2022

Proteomics

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We believe this entire collection presents a wide overview of topics that are of growing interest to the nanopore sensing community. We have focused this issue on both biosensing applications of nanopore detection and fundamental mechanisms underlying polymer-pore interactions. We have identified a variety of investigators active in these pursuits and we hope that this will help further motivate studies in these areas. We thank each of the authors for their efforts along with the reviewers for making this special issue a reality, and we hope you, the reader, will benefit from the studies reported herein.

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“…Carbon nanosheet possessed tremendous energy-power characteristics from hybrid sodium-ion capacitors . In addition, PEO or polyethylene glycol (PEG)-based materials could be used as additives to form crystalline phase at room temperature with stability of sodium metal, lightweight, and low interfacial resistance as well as to form nanopores by PEG partitioning. − Moreover, the Mxene (M n +1 X n T x ) family, in which T x stands for terminal surface groups −O, −F, and/or −OH, owns an outstanding metallic conductivity with tunable surface and intercalation/deintercalation chemistry that contributes for enhancing capacitance . For example, the sandwich structure of Ti 3 C 2 T x Mxene formed with polyethylenimine improved the capability of Na + storage via a hydrogen-bonding network …”

Section: Introductionmentioning

confidence: 99%

Multiplex Wearable Electrochemical Sensors Fabricated from Sodiated Polymers and Mxene Nanosheet To Measure Sodium and Creatinine Levels in Sweat

Kalasin,

Sangnuang

2023

ACS Appl. Nano Mater.

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Noninvasive multifunctional wearable technology with long-term care provides a promising future for personalized and remote health assessment that enables delay or mitigate epidemiology of multimorbidity, which is susceptible in aging societies. The new generation of textile electrodes (TEs) that include capacitive transducers gains outstanding performance with stable signal monitoring capable of long-term healthcare analysis. Nevertheless, one limitation is that the existing coarseness phase during superior sensing material (SM) deposition could degrade the sensing performance over time. This paper addresses sodium-ion and creatinine sensing integrated with epidermal wearable sensors that focused on an unexplored property of electrochemical solid-contact sodiated transducers made of sodiated-redox-active conductive polymers, poly(3,4-ethylenedioxythiophene) polystyrenesulfonate (PEDOT:PSS) (for potentiometric sodium-ion sensing) and polypyrrole (PPy) (for voltammetry creatinine sensing), which was sandwiched between sodiated nanoporous-layer carbon-polyethylene glycol and sodiated Ti 3 C 2 T x Mxene nanosheet. A high stable current density for 3,000 cycles demonstrated that the sodiated nanosheet minimized an electron transfer loss via nanoporous ethylene layer, redox-active PEDOT:PSS and PPy by exploiting the sodium dopant. A potentiometric transducer achieved 428 μF and signal drift of 0.02 mV/h, while a voltammetric transducer achieved 402 μF with current drift of 0.008 μA/h (within 0.3 μM/h) in 24 h. The degradation of potential and current drifts was within 0.01 mV/h and 0.01 μA/h for 6 months. The integrated electrochemical TEs produced outstanding stretching cycles and potential retention, advantageous for long-term diagnostics while combining the minimizing-interference layer to reduce sweat interference. The wearable multifunctionality with minimized degradation of SMs was demonstrated in real-time with dynamic sweat analysis related to routine activities. Hence, this wearable sensor enabled the information on life-threatening events that could minimize or prevent long-term health organ failure at sodiumion and creatinine metabolite variations as well as facilitate skin and environmental temperature determination in times of epidemiology of multimorbidity.

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Probing protein nanopores with poly(ethylene glycol)s

Cited by 5 publications

References 116 publications

Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure

Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure

Highlights on the current state of proteomic detection and characterization with nanopore sensors

Multiplex Wearable Electrochemical Sensors Fabricated from Sodiated Polymers and Mxene Nanosheet To Measure Sodium and Creatinine Levels in Sweat

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