Probing redox reaction of azurin protein immobilized on hydroxyl-terminated self-assembled monolayers with different lengths

Hamzehloei, Ali; Bathaie, S. Zahra; Mousavi, Mir F.

doi:10.1016/j.jelechem.2015.07.005

Cited by 7 publications

(5 citation statements)

References 37 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is ascribed to the location of the copper centre being far from the electrode and buried inside the protein matrix. 84 Square wave voltammogram of bare Si−H electrode did not show any redox peaks but rather a sharp increase in the current reflecting significant Si oxidation in the absence of the protein monolayer (Figure 7b).…”

Section: Protein Azurin On Simentioning

confidence: 97%

Covalent Linkages of Molecules and Proteins to Si–H Surfaces Formed by Disulfide Reduction

et al. 2020

View full text Add to dashboard Cite

Thiols and disulfides contacts have been, for decades, key for connecting organic molecules to surfaces and nanoclusters as they form self-assembled monolayers (SAMs) on metals such as gold (Au) under mild conditions. In contrast, they have not been similarly deployed on Si owing to the harsh conditions required for monolayers formation. Here, we show that SAMs can be simply formed by dipping Si−H surfaces into dilute solutions of organic molecules or proteins comprising disulfide bonds. We demonstrate that S−S bonds can be spontaneously reduced on Si−H, forming covalent Si−S bonds in the presence of traces of water, and that this grafting can be catalyzed by electrochemical potential. Cyclic disulfide can be spontaneously reduced to form complete monolayers in 1 hour and the reduction can be catalyzed electrochemically to form full surface coverages within 15 minutes. In contrast, the kinetics of SAM formation of the cyclic disulfide molecule on Au was found to be three folds slower than that on Si. It is also demonstrated that dilute thiol solutions can form monolayers on Si−H following oxidation to disulfides under ambient conditions; the supply of too much oxygen, however, inhibits SAM formation. The electron-transfer kinetics of the Si-S enabled SAMs on Si-H is comparable to that on Au SAMs, suggesting that Si−S contacts are electrically transmissive. We further demonstrate the prospective of this spontaneous disulfide reduction by forming a monolayer of protein azurin on a Si-H surface within 1 hour. The direct reduction of disulfides on Si electrodes opens new capabilities for a range of fields, including molecular electronics, for which highly conducting SAM-electrode contacts are necessary, and for emerging fields such as bio-molecular electronics as disulfide linkages could be exploited to wire proteins between Si electrodes, within context of the current Si-based technologies.

show abstract

Section: Protein Azurin On Simentioning

confidence: 97%

Covalent Linkages of Molecules and Proteins to Si–H Surfaces Formed by Disulfide Reduction

et al. 2020

View full text Add to dashboard Cite

show abstract

“…One emphasis of biological SECM measurements has been the characterization of proteins and their building blocks. Several different systems have been investigated in the past five years, including tryptophan (amino acid), 402 Tat(44-57) and melittin (peptides), 403 azurin (protein), 404 bacterial flaggelin (protein), 405 cytochrome c (protein), 406 ovalbumin (protein), 407 and photosystem I (protein complex). 193 An interesting "real world" application involving proteins has been the analysis of latent fingerprints on various surfaces.…”

Section: Biologicalmentioning

confidence: 99%

“…One emphasis of biological SECM measurements has been the characterization of proteins and their building blocks. Several different systems have been investigated in the past five years, including tryptophan (amino acid), Tat(44-57) and melittin (peptides), azurin (protein), bacterial flaggelin (protein), cytochrome c (protein), ovalbumin (protein), and photosystem I (protein complex) . An interesting “real world” application involving proteins has been the analysis of latent fingerprints on various surfaces. − For example, Zhang and co-workers used direct current magnetron sputtering (based on vacuum metal deposition) to deposit a thin conductive film of Al-doped ZnO (ZAO) onto a surface containing a latent fingerprint .…”

Section: Applicationsmentioning

confidence: 99%

Scanning Electrochemical Microscopy: A Comprehensive Review of Experimental Parameters from 1989 to 2015

2016

View full text Add to dashboard Cite

Scanning electrochemical microscopy (SECM) is an electroanalytical scanning probe technique capable of imaging substrate topography and local reactivity with high resolution. Since its inception in 1989, it has expanded into a wide variety of research areas including biology, corrosion, energy, kinetics, instrumental development, and surface modification. In the past 25 years, over 1800 peer-reviewed publications have focused on SECM, including several topical reviews. However, these reviews often omit key details, forcing readers to search the literature. In this review, we provide a comprehensive summary of the experimental parameters (e.g., solvents, probes, and mediators) used in all SECM publications since 1989, irrespective of the application. It can be used to rapidly assess experimental possibilities and make an informed decision about experimental design. In other words, it is a practical guide to SECM.

show abstract

“…The shift of initial oxidation potential and reduction peak potential of CAT and HQ with pH values indicated that the protons were directly involved in the redox reaction of CAT and HQ. 24,41 According to the pK a (>10) of hydroxyl group from MCH, 29 and the pK a of CAT (9.3, 13.0), HQ (10,12) and RE (9.4, 12.3), 42 the CAT, HQ and RE were stable with two hydroxyl groups existed especially at pH 4.4 and 7.0. Thus, the discrimination effect of CAT and HQ might be similar at pH 4.4 and 7.0.…”

Section: Comparison Ofmentioning

confidence: 99%

“…Understanding the difference of different functional groups (-COOH, -OH or -NH 2 ) on discriminating CAT, HQ and RE is important for designing sensor with good performance. Functional groups (-COOH, -OH or -NH 2 ) terminated thiols could adsorb on gold to form self-assembled monolayers (SAMs), [27][28][29] which provided the ideal interface for exploring the effect of functional groups on the performance of sensors. It is necessary to investigate the discrimination effect of CAT, HQ and RE based on functional groups (-COOH, -OH or -NH 2 ) terminated thiols SAMs on gold.…”

mentioning

confidence: 99%

Electrochemical Discrimination of Dihydroxybenzene Isomer at Different Thiols Self-Assembled Monolayers on Gold and Simultaneous Determination of Catechol and Hydroquinone

Liu

Jia

et al. 2020

J. Electrochem. Soc.

View full text Add to dashboard Cite

The discrimination effect of dihydroxybenzene isomer including catechol (CAT), hydroquinone (HQ) and resorcinol (RE) on bare gold, and a series of thiols self-assembled monolayers (SAMs) including mercaptohexanol (MCH), 6-mercaptohexanoic acid (MHA), mercaptoethylamine (MEA), thiol-modified double-stranded DNA (ds-DNA), single-stranded DNA (ss-DNA) is first investigated by cyclic voltammetry (CV) and linear scan voltammetry (LSV). Results show that CAT, HQ and RE can not be electrochemically discriminated simultaneously by bare gold or thiols SAMs based on their oxidation or reduction peaks. Furthermore, RE has a big interference on the electrochemical response of CAT and HQ. At acidic solution (pH 4.4), in presence of RE, CAT and HQ can not be discriminated on bare gold, ds-DNA or ss-DNA SAMs. However, CAT and HQ can be discriminated well at MCH, MHA, and MEA SAMs on gold based on the difference of reduction peak potentials (ΔE pc) as 219, 212 and 165 mV respectively. MCH SAMs is chosen to study simultaneous determination of CAT and HQ due to bigger ΔE pc and current response. We fix the potential at 0.7 V for 60 s to enrich the oxidation products of CAT and HQ, then investigated the relationships of reduction peak current (i pc) with the concentrations of CAT and HQ. The i pc values are linear with the concentrations of CAT and HQ in the range of 7.0 × 10−6∼1.04 × 10−3 M. The limit of detection (LOD) is calculated to be 5.9 × 10−7 M for CAT and 8.3 × 10−7 M for HQ (S/N = 3). The MCH SAMs on gold is applied to the simultaneous determination of CAT and HQ in lake water sample and satisfactory result is obtained.

show abstract

Probing redox reaction of azurin protein immobilized on hydroxyl-terminated self-assembled monolayers with different lengths

Cited by 7 publications

References 37 publications

Covalent Linkages of Molecules and Proteins to Si–H Surfaces Formed by Disulfide Reduction

Covalent Linkages of Molecules and Proteins to Si–H Surfaces Formed by Disulfide Reduction

Scanning Electrochemical Microscopy: A Comprehensive Review of Experimental Parameters from 1989 to 2015

Electrochemical Discrimination of Dihydroxybenzene Isomer at Different Thiols Self-Assembled Monolayers on Gold and Simultaneous Determination of Catechol and Hydroquinone

Contact Info

Product

Resources

About