Probing the binding of trypsin to glutathione-stabilized gold nanoparticles in aqueous solution

Wang, Gongke; Liu, Xingbing; Yan, Changling; Bai, Guangyue; Lü, Yan

doi:10.1016/j.colsurfb.2015.07.063

Cited by 22 publications

(19 citation statements)

References 29 publications

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“…From Table 2, we can nd that DH 0 is a negative value, while DS 0 is a positive value, which demonstrates that the main acting forces during the interaction between the AgNPs and BSA may be hydrogen bonding and hydrophobic interactions. 29 Furthermore, it is seen that the positive value of DS 0 and the negative value of DH 0 can cooperatively contribute to the negative value of DG 0 . Therefore, it seems to be implied that the binding of BSA to AgNPs is synergistically driven by enthalpy and entropy.…”

Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 90%

“…However, for the static quenching mode, the quenching constant will decrease at higher temperatures. 29,30 In our case, the concentration of BSA was constant at 1.0 mM, and the AgNPs were added to a maximal concentration (10 À10 M). Fig.…”

Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 99%

“…In order to reveal energetic changes in the binding of BSA with AgNPs, the thermodynamic parameters of the binding process were further analyzed using the Van't Hoff equation. If the thermodynamic parameters DH 0 (change in enthalpy) and DS 0 (change in entropy) experience little change over the temperature range, then DH 0 and DS 0 can be derived from the following equation: 29,32 ln…”

Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 99%

“…2B), the emission peaks of the tryptophan (Trp) residues had almost no change, suggesting that the interaction between BSA and the AgNPs did not affect the microenvironment of the Trp residues. 29,32 FT-IR spectroscopy can be adopted to directly analyze the effect of the AgNPs on the secondary structures of BSA. The FT- IR spectra of the protein exhibit a number of amide bands, which represent the different vibrations of the peptide strands.…”

Section: Conformational Changes Of the Proteinmentioning

confidence: 99%

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Probing the binding behavior and kinetics of silver nanoparticles with bovine serum albumin

Wang

Hou

et al. 2017

RSC Adv.

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Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 90%

Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 99%

Section: Mechanism Of Uorescence Quenching and Binding Modementioning

confidence: 99%

Section: Conformational Changes Of the Proteinmentioning

confidence: 99%

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Probing the binding behavior and kinetics of silver nanoparticles with bovine serum albumin

Wang

Hou

et al. 2017

RSC Adv.

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“…Additionally, the data were fitted with a modified Hill equation, obtaining the following relation (2) 42,43 where:…”

Section: Methodsmentioning

confidence: 99%

Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism

Peinado

Olivier

Eberle

et al. 2019

Sci Rep

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Vitamin B 12 acts as a cofactor for various metabolic reactions important in living organisms. The Vitamin B 12 biosynthesis is restricted to prokaryotes, which means, all eukaryotic organisms must acquire this molecule through diet. This study presents the investigation of Vitamin B 12 metabolism and the characterization of precorrin-4 C(11)-methyltransferase (CobM), an enzyme involved in the biosynthesis of Vitamin B 12 in Corynebacterium pseudotuberculosis . The analysis of the C . pseudotuberculosis genome identified two Vitamin B 12 -dependent pathways, which can be strongly affected by a disrupted vitamin metabolism. Molecular dynamics, circular dichroism, and NMR-STD experiments identified regions in CobM that undergo conformational changes after s-adenosyl-L-methionine binding to promote the interaction of precorrin-4, a Vitamin B 12 precursor. The binding of s-adenosyl-L-methionine was examined along with the competitive binding of adenine, dATP, and suramin. Based on fluorescence spectroscopy experiments the dissociation constant for the four ligands and the target protein could be determined; SAM (1.4 ± 0.7 µM), adenine (17.8 ± 1.5 µM), dATP (15.8 ± 2.0 µM), and Suramin (6.3 ± 1.1 µM). The results provide rich information for future investigations of potential drug targets within the C . pseudotuberculosis’s Vitamin B12 metabolism and related pathways to reduce the pathogen’s virulence in its hosts.

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Synthesis and characterization of molecularly imprinted polymer nanoparticles against porcine circovirus type 2 viral-like particles

Klangprapan,

Sudjarwo,

Lieberzeit

et al. 2024

Anal Bioanal Chem

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PCV2 is a significant epidemic agricultural pathogen that causes a variety of swine diseases. PCV2 infections have significant economic impact on the swine industry, making effective strategies for rapid detection of PCV2 in pigs essential. Herein, we report on the synthesis of the so-called nano-MIPs which can be utilized for molecular recognition of PCV2. The morphology and structure of nano-MIPs were characterized using scanning electron microscopy (SEM). Nano-MIPs are spherical with sizes around 120–150 nm. Binding experiments demonstrate that the fluorescence intensity of PCV2 samples decreases proportionally to increasing the concentration of nano-MIPs due to quenching, while non-imprinted polymer nanoparticles (nano-NIPs) do not affect the signal. The Stern–Volmer constant of nano-MIPs binding to PCV2 was 1.3 × 10−3 mL/µg, whereas nano-NIPs led to 7 × 10−5 mL/µg, i.e., 1.8 orders of magnitude lower. The detection limit for binding MIP particles to PCV2 by fluorescence measurements is 47 µg/mL. This affinity test allows for designing both direct and competitive quartz crystal microbalance (QCM) assays for PCV2 leading to QCM measurements. The QCM results show nano-MIPs binding to PCV2 immobilized on the sensor surface with appreciable reproducibility. QCM sensor characteristics reveal signal saturation above around 200 µg/mL at a response of − 354 Hz and an LOD of approximately 35 µg/mL. Nano-MIPs also show selectivity factors of 2–5 for CSFV and PRRSV probably because the three viruses have similar diameters around 50 nm. Graphical Abstract

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Probing the binding of trypsin to glutathione-stabilized gold nanoparticles in aqueous solution

Cited by 22 publications

References 29 publications

Probing the binding behavior and kinetics of silver nanoparticles with bovine serum albumin

Probing the binding behavior and kinetics of silver nanoparticles with bovine serum albumin

Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism

Synthesis and characterization of molecularly imprinted polymer nanoparticles against porcine circovirus type 2 viral-like particles

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