Probing the determinants of phosphorylated sugar-substrate binding for human sialic acid synthase

Cotton, Thomas R.; Joseph, Dmitri D. A.; Jiao, Wanting; Parker, Emily J.

doi:10.1016/j.bbapap.2014.09.014

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…It contrasts with second-order factorial modelling, where items cannot load onto the general factor directly, but only through their respective factors 14 THE SPANISH-CHILEAN-TEIQUE-SF (see Chen, West, & Sousa, 2006;Reise, 2012). Although researchers in cognitive assessment have extensively studied bi-factor models (Gignac & Watkins, 2013;Gustafsson & Balke, 1993;Holzinger & Swineford, 1937;Luo, Petrill & Thompson, 1994), their applicability has also proved useful in the psychometric assessment of non-cognitive constructs (Morin et al, 2015).…”

Section: The Spanish-chilean-teique-sfmentioning

confidence: 99%

The Spanish-Chilean Trait Emotional Intelligence Questionnaire-Short Form: The Adaptation and Validation of the TEIQue-SF in Chile

Pérez-Díaz

Petrides

2019

Journal of Personality Assessment

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There is little doubt that currently trait EI (Trait emotional intelligence) theory and their measures have been found valid and reliable in several research and application settings. This research provides psychometric evidence of the TEIQue-SF in Chilean general and clinical population sample (n₁ = 335, n₂ = 120). The results confirmed the factor structure of the instrument and supported its multidimensionality. Hierarchical and bi-factor CFA models with the Spanish-Chilean-TEIQue-SF analysed its internal structure in the R environment, following the assessment of bi-factor ESEM models in Mplus. We performed these analyses both at the global and factorlevel. CFA models did not reach acceptable fit statistics for the models, whereas ESEM models reached good to excellent fit for the bi-factor models proposed. We also implemented measurement invariance analyses, which provided evidence for full measurement invariance between the original UK validation sample and the Chilean samples up to the scalar level. Also, the means for the global trait EI factor and the four factors (Well-being, Self-control, Emotionality and Sociability) were alike previous psychometric research with the questionnaire. The results highlight the importance of performing multidimensional factor analysis through ESEM following a bi-factor interpretation of the TEIQue-SF internal structure. 3 THE SPANISH-CHILEAN-TEIQUE-SF The study of Emotional Intelligence (EI) in Chile and Latin-America Although some studies have addressed EI in Chile, either their nature do not fit with the taxonomies already in place, or they suffer from methodological flaws. Therefore, addressing EI scientifically, with well-defined construct operationalisations, is eventually the only pathway for assessing the subjectivity of emotional experience (Petrides, 2009). Current research on emotional intelligence in Chile and Latin-America has usually not responded to psychometrically founded constructs such as trait EI. At most, local research has relied on trait EI scales of which no previous supporting psychometric evidence has been reported in the country. The latter is a serious limitation for assessing trait EI accurately not only in Chile but also in the region. For instance, some studies in the country have relied on the Spanish adaptation of the TMMS-48 (Trait Meta-Mood Scale-Spanish translation), performed in the late nineties in Spain (Fernández-Berrocal et al., 1998). Fernández-Berrocal, Salovey, Vera, Ramos and Extremera (2001) conducted a study based on this questionnaire four measures of emotional stability, where participants of U.S.A., Spain and Chile were compared cross-culturally regarding their trait EI and emotional stability. The authors concluded that the Spanish sample had a significantly lowest mean for trait EI compared to the U.S.A. and the Chilean samples. This latter also obtained the highest score for emotional balance. Although these authors did not inform measurement invariance between the samples, it can be deducted from the significant differenc...

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Section: The Spanish-chilean-teique-sfmentioning

confidence: 99%

The Spanish-Chilean Trait Emotional Intelligence Questionnaire-Short Form: The Adaptation and Validation of the TEIQue-SF in Chile

Pérez-Díaz

Petrides

2019

Journal of Personality Assessment

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“…The main difference is in the phosphorylation of the substrate and the product. NanS uses ManNAc-6-phosphate instead of ManNAc and produces Neu5Ac-9-phosphate instead of Neu5Ac, a reaction favoured by the interaction with the α2β2 loop present in the human enzyme [ 18 ]. The phosphate of Neu5Ac-9-phosphate is subsequently removed by the NanP phosphatase [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis

2021

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Nonulosonic acids (NulOs) are a group of nine-carbon monosaccharides with different functions in nature. N-acetylneuraminic acid (Neu5Ac) is the most common NulO. It covers the membrane surface of all human cells and is a central molecule in the process of self-recognition via SIGLECS receptors. Some pathogenic bacteria escape the immune system by copying the sialylation of the host cell membrane. Neu5Ac production in these bacteria is catalysed by the enzyme NeuB. Some bacteria can also produce other NulOs named pseudaminic and legionaminic acids, through the NeuB homologues PseI and LegI, respectively. In Opisthokonta eukaryotes, the biosynthesis of Neu5Ac is catalysed by the enzyme NanS. In this study, we used publicly available data of sequences of NulOs synthases to investigate its distribution within the three domains of life and its relationship with pathogenic bacteria. We mined the KEGG database and found 425 NeuB sequences. Most NeuB sequences (58.74 %) from the KEGG orthology database were classified as from environmental bacteria; however, sequences from pathogenic bacteria showed higher conservation and prevalence of a specific domain named SAF. Using the HMM profile we identified 13 941 NulO synthase sequences in UniProt. Phylogenetic analysis of these sequences showed that the synthases were divided into three main groups that can be related to the lifestyle of these bacteria: (I) predominantly environmental, (II) intermediate and (III) predominantly pathogenic. NeuB was widely distributed in the groups. However, LegI and PseI were more concentrated in groups II and III, respectively. We also found that PseI appeared later in the evolutionary process, derived from NeuB. We use this same methodology to retrieve sialic acid synthase sequences from Archaea and Eukarya. A large-scale phylogenetic analysis showed that while the Archaea sequences are spread across the tree, the eukaryotic NanS sequences were grouped in a specific branch in group II. None of the bacterial NanS sequences grouped with the eukaryotic branch. The analysis of conserved residues showed that the synthases of Archaea and Eukarya present a mutation in one of the three catalytic residues, an E134D change, related to a Neisseria meningitidis reference sequence. We also found that the conservation profile is higher between NeuB of pathogenic bacteria and NanS of eukaryotes than between NeuB of environmental bacteria and NanS of eukaryotes. Our large-scale analysis brings new perspectives on the evolution of NulOs synthases, suggesting their presence in the last common universal ancestor.

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“…SA is bound to the tip of glycan systems, if CMP-sialic acid is available [34]. This substance is built up along a well-known pathway with sialic acid synthase (NANS) as the key enzyme [35]. Linked to a CMP, SA is transported to its destination point, where it is transferred to the tip of an existing glycan structure [36].…”

Section: Introductionmentioning

confidence: 99%

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

Bauer

Gombocz²,

Wehland

et al. 2020

IJMS

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The adhesion behavior of human tissue cells changes in vitro, when gravity forces affecting these cells are modified. To understand the mechanisms underlying these changes, proteins involved in cell-cell or cell-extracellular matrix adhesion, their expression, accumulation, localization, and posttranslational modification (PTM) regarding changes during exposure to microgravity were investigated. As the sialylation of adhesion proteins is influencing cell adhesion on Earth in vitro and in vivo, we analyzed the sialylation of cell adhesion molecules detected by omics studies on cells, which change their adhesion behavior when exposed to microgravity. Using a knowledge graph created from experimental omics data and semantic searches across several reference databases, we studied the sialylation of adhesion proteins glycosylated at their extracellular domains with regards to its sensitivity to microgravity. This way, experimental omics data networked with the current knowledge about the binding of sialic acids to cell adhesion proteins, its regulation, and interactions in between those proteins provided insights into the mechanisms behind our experimental findings, suggesting that balancing the sialylation against the de-sialylation of the terminal ends of the adhesion proteins’ glycans influences their binding activity. This sheds light on the transition from two- to three-dimensional growth observed in microgravity, mirroring cell migration and cancer metastasis in vivo.

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Probing the determinants of phosphorylated sugar-substrate binding for human sialic acid synthase

Cited by 4 publications

References 35 publications

The Spanish-Chilean Trait Emotional Intelligence Questionnaire-Short Form: The Adaptation and Validation of the TEIQue-SF in Chile

The Spanish-Chilean Trait Emotional Intelligence Questionnaire-Short Form: The Adaptation and Validation of the TEIQue-SF in Chile

Origin and evolution of nonulosonic acid synthases and their relationship with bacterial pathogenicity revealed by a large-scale phylogenetic analysis

Insight in Adhesion Protein Sialylation and Microgravity Dependent Cell Adhesion—An Omics Network Approach

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