Probing the differential effects of infrared light sources IR1072 and IR880 on human lymphocytes: Evidence of selective cytoprotection by IR1072

Bradford, Andrea M.; Barlow, Amelia; Chazot, Paul L.

doi:10.1016/j.jphotobiol.2005.05.005

Cited by 31 publications

(24 citation statements)

References 27 publications

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“…Human peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA blasts) were generated essentially as described previously (Bradford et al, 2005). In brief, heparinized human whole blood was obtained from healthy volunteers (with local ethical approval), and PBMCs were separated using Lymphoprep (Axis-Shield Poc AS, Oslo, Norway) and centrifuged at 400g for 25 min.…”

Section: Construction Of Epitope-tagged Proteins For Tr-fretmentioning

confidence: 99%

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors

Rijn¹,

Chazot²,

Shenton³

et al. 2006

Mol Pharmacol

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In this study, we report the homo-and hetero-oligomerization of the human histamine H 4 R by both biochemical (Western blot and immobilized metal affinity chromatography) and biophysical [bioluminescence resonance energy transfer and time-resolved fluorescence resonance energy transfer (tr-FRET)] techniques. The H 4 R receptor is the most recently discovered member of the histamine family of G-protein-coupled receptors. Using specific polyclonal antibodies raised against the C-terminal tail of the H 4 R, we demonstrate the presence of H 4 R oligomers in human embryonic kidney 293 and COS-7 cells heterologously overexpressing H 4 Rs and putative native H 4 R oligomers in human phytohaemagglutinin blasts endogenously expressing H 4 Rs. Moreover, we show that H 4 R homo-oligomers are formed constitutively, are formed at low receptor densities (300 fmol/mg of protein), and are present at the cell surface, as detected by tr-FRET. The formation of these oligomers is independent of N-glycosylation and is not modulated by H 4 R ligands, covering the full spectrum of agonists, neutral antagonists, and inverse agonists. Although we show H 4 R homo-oligomer formation at physiological expression levels, the detection of H 1 R-H 4 R hetero-oligomers was achieved only at higher H 1 R expression levels and are most likely not physiologically relevant.

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Section: Construction Of Epitope-tagged Proteins For Tr-fretmentioning

confidence: 99%

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors

Rijn¹,

Chazot²,

Shenton³

et al. 2006

Mol Pharmacol

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“…Nitric oxide (NO) in low concentrations is known to be a potent inhibitor of apoptosis in various cell types . The authors suggested that the increase in iNOS might be the underlying mechanism of protective preconditioning by 1072 nm light therapy in human lymphocytes . Considering this, we can hypothesize that 1072 nm infrared light therapy may enhance the cell viability of lymphocytes that act against HSV infection.…”

Section: Discussionmentioning

confidence: 99%

“…An in vitro study by Bradford et al found that 1072 nm infrared LLLT elicited a significant protective effect on human lymphocytes. 22 In their study, the cell viability of peripheral blood mononuclear cells (PBMCs) after exposure to ultraviolet (UV) light was significantly increased when cells were pretreated with 1072 nm light before the UV exposure (preconditioning). Quantitative immunoblotting showed that there was a significant 4.9-fold increase in the level of iNOS in the pretreated group compared with the control group.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the efficacy of low-level light therapy using 1072 nm infrared light for the treatment of herpes simplex labialis

Dougal¹,

Lee²

2013

Clin Exp Dermatol

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“…[9][10][11][12] E®ects such as cytoprotection, cellular proliferation, growth factor release have also been reported. [13][14][15] Studies have indicated the e®ects of NIR on nervous system, like AD. [16][17][18][19] The biological e®ects of NIR are speci¯c to wavelength and not based on thermal e®ects.…”

Section: Introductionmentioning

confidence: 99%

Near infra-red light treatment of Alzheimer’s disease

Han

Wang

Xue

et al. 2017

J. Innov. Opt. Health Sci.

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Alzheimer's disease (AD) is a chronic neurodegenerative disease. The symptoms include memory and spatial learning di±culties, language disorders, and loss of motivation, which get worse over time, eventually ending in death. No e®ective treatments are available for AD, currently. Current treatments only attenuate symptoms temporarily and are associated with severe side e®ects. Near infra-red (NIR) light has been studied for a long time. We investigated the e®ect of NIR on AD using a transgenic mouse model, which was obtained by co-injecting two vectors carrying AD mutations in amyloid precursor protein (APP) and presenilin-1 (PSEN1) into C57BL/6J mice. The irradiation equipment consisted of an accommodating box and an LED array. The wavelength of NIR light emitted from LED was between 1040 nm and 1090 nm. The power density delivered at the level of the mice was approximately 15 mW/cm 2 . Firstly, we treated the mice with NIR for 40 days. Then, the irradiation was suspended for 28 days. Finally, another 15 days treatment was brought to mice. We conducted Morris water maze and immuno°uorescence analysis to evaluate the e®ects of treatment. Immuno°uorescence analysis was based on measuring the quantity of plaques in mouse brain slices. Our results show that NIR light improves memory and spatial learning ability and reduces plaques moderately. NIR light represents a potential treatment for AD.

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Probing the differential effects of infrared light sources IR1072 and IR880 on human lymphocytes: Evidence of selective cytoprotection by IR1072

Cited by 31 publications

References 27 publications

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors

Evaluation of the efficacy of low-level light therapy using 1072 nm infrared light for the treatment of herpes simplex labialis

Near infra-red light treatment of Alzheimer’s disease

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Probing the differential effects of infrared light sources IR1072 and IR880 on human lymphocytes: Evidence of selective cytoprotection by IR1072

Cited by 31 publications

References 27 publications

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H4Receptors

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H4Receptors

Evaluation of the efficacy of low-level light therapy using 1072 nm infrared light for the treatment of herpes simplex labialis

Near infra-red light treatment of Alzheimer’s disease

Contact Info

Product

Resources

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Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors

Oligomerization of Recombinant and Endogenously Expressed Human Histamine H₄Receptors