Probing the limitations of the fluorous content for tag-mediated microarray formation

Chemistry of natural glycan microarrays

“…It is likely that all of these products show slight variations in the actual microarray experimental results. Printing platforms such as nitrocellulose [34,57,58] and fluorous slides [59,60] present more complex chemistry/physics in glycan immobilization, generally thought to be through hydrophobic interactions requiring addition of a lipid to the glycans that normally lack them. A potential merit of nitrocellulose membranes is the existence of three-dimensional structure, compared to the two-dimensional surface of other glass surfaces.…”

Fluorous enzymatic synthesis of phosphatidylinositides

“…1) where tandem enzymatic reactions are used to generate multiple probes after purification through fluorous solid phase extraction (FSPE) [8a] . These probes can then be used as enzyme reporters, or be directly immobilized on a fluorous surface to form a microarray [11] to investigate protein-small molecule interactions. PtdIns(4,5)P 2 is the most well-studied PI and functions as a substrate of multiple enzymes including phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC).…”

“…We chose to use Teflon as the fluorous surface because the fluorous array used in the literature [11] is no longer commercially available. Equal molar amounts of the three fluorous tagged PIs, PtdIns(4,5)P 2 , PtdIns(3,4,5)P 3 , and PtdIns(3,4)P 2 , from the enzymatic reactions were spotted on a perfluorinated Teflon membrane for immobilization.…”

Application of Fluorous Chemistry for Oligosaccharide Synthesis

“…Since then various oligosaccharide syntheses with the fluorous method have been described (8)(9)(10)(11)(12). We have also reported peptide and oligosaccharide synthesis by using various fluorous tags, the molecules of which contain polyamide bonds (Scheme 2) (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).…”