Probiotic capability of novel lactic acid bacteria isolated from worker honey bees gut microbiota

El-Zeini, Hoda M.; Ali, Abdel Rahman Abdel Atti; Nasr, Nasr Fawzy; Hassan, Mariam; Hassan, Ashwaa Abdel Moneim; Elenany, Yasser Essam

doi:10.1093/femsle/fnab030

Cited by 22 publications

(18 citation statements)

References 25 publications

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“…Такі препарати не адаптовані до бджіл, змінюють склад мікробіому цих комах, що негативно відображається на їх здоров'ї (Kwong et al, 2017;Leonard et al, 2018;Zheng et al, 2018). Тому важливою характеристикою пробіотичних засобів є специфічність дії щодо виду тварин (Bomilla-Rosso & Engel, 2018;Elzeini et al, 2021;Hordiienko et al, 2022).…”

Section: вступunclassified

“…Продуктивність бджолиної сім'ї безпосередньо залежить від її сили, а також від впливу ряду екологічних чинників, серед яких забруднення навколишнього середовища, наявність збудників інфекційних та інвазійних захворювань, застосування антибіотиків тощо. Для отримання екологічно безпечної продукції бджільництва (наприклад, меду без антибіотиків), стимуляції розмноження та підвищення резистентності бджіл до збудників інфекційних захворювань і несприятливих чинників довкілля назріла доцільність застосовувати комплексні натуральні препарати, зокрема пробіотики (Olofsson et al, 2016;Elzeini et al, 2021;Hordiienko et al, 2022).…”

Section: обговоренняunclassified

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Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

Postoienko¹,

Postoienko²,

Yefimenko³

et al. 2022

SMLNUVMB

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Viral diseases of bees, in particular the sac brood, are an urgent problem of the industry, which brings great losses. The situation worsens in the case of parallel infestation of insects by the Varroa mite. Today, a complex approach is used for the treatment of viral diseases of bees, which is based on a connection of antiviral and antiparasitic measures in combination with drugs that have general strengthening and biostimulating effects. Nevertheless, the issue of developing ecologically safe and effective schemes for the prevention and treatment of bee diseases, in particular viral ones, is relevant today. In this work, the positive effect of using a specific probiotic for bees “Apinormin” for sac brood was investigated. Research in laboratory conditions was carried out on bees of the summer generation, selected into cages. The oral effect of the drug was determined by feeding bees with sugar syrup, the contact effect by spraying the body of insects with an aqueous solution in concentrations of 0.4; 0.8; 4.0; 8.0 %. Under natural conditions, the therapeutic effectiveness of a 4 % solution of “Apinormin” compared to the creation of an infertile period for the period of removal of queens for bee sac brood was determined. In laboratory conditions, the drug was proven to be harmless for bees when used orally in all tested concentrations. It has been established that “Apinormin” significantly increases the life expectancy of insects. Thus, feeding an 8 % probiotic solution slowed down the process of natural dying of bees from 7.24 to 27.75 % in the dynamics of observations. Under natural conditions, the drug did not show an antiviral effect. The effectiveness of the infertile period in the treatment of this viral disease is shown. The obtained positive effect of “Apinormin” on the life extension of bees and the presence of a number of biologically active compounds in its composition serve as a basis for the use of the drug to increase the strength and productivity of families, in case of stress, protein starvation, various technological violations of maintenance and the development of schemes for the prevention and treatment of bacterial diseases of bees. The expediency of using the infertile period for the period of removal of the queens in the following studies, both for self-administration and for working out the schemes of complex therapy of infectious diseases of bees together with the appropriate veterinary drugs, is substantiated.

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Section: вступunclassified

Section: обговоренняunclassified

Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

Postoienko¹,

Postoienko²,

Yefimenko³

et al. 2022

SMLNUVMB

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“…Високу активність α-амілази реєструємо у бджіл, які споживали пробіотик, розведений медовим гречаним сиропом (5 % -5577 ОД/л; 1,25 % -4608 ОД/л). Така активність зумовлена здатністю молочнокислих бактерій зі складу препарату продукувати комплекс травних ферментів, зокрема амілазу (Elzeini et al, 2021).…”

Section: матеріал і методи дослідженьunclassified

Effect of “EM® PROBIOTIC FOR BEES” on the biochemical parameters of bee haemolymph in an entomological cage experiment

Лахман¹,

Galatiuk²,

Романишина³

et al. 2022

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The problem of proper support for the health of bee colonies is now a topical issue. The study of bee haemolymph parameters under the influence of certain agents and factors has been carried out by a number of scientists in different countries of the world. Therefore the determination of changes in biochemical parameters of bee hemolymph when applying different concentrations (5 %, 2.5 %, 1.25 %) of “EM® PROBIOTIC FOR BEES” diluted with honey buckwheat and sugar syrup was the aim of the experiment. The study included placing experimental insects in entomological cages, creating optimal conditions for their life, feeding them with “EM® PROBIOTIC FOR BEES” diluted with honey buckwheat and sugar syrup in different concentrations and studying biochemical parameters of their haemolymph on the “limiting” day in relation to the time of their death. Some biochemical parameters of the bees from 8 experimental groups (laboratory conditions) were compared with the hemolymph Apis mellifera of the control (natural conditions) group from the same apiary. Biochemical parameters of haemolymph: total protein (PRO); albumin (ALB); glucose (GLU); α-amylase (AMY); alkaline phosphatase (ALP); aspartate aminotransferase (AST) (SGOT); gamma-glutamyl transpeptidase (GGT) and creatinine (Cre) were determined by a Chem 7 semiautomatic biochemical analyser by wavelength selection and calibration for each parameter. The changes in these parameters in relation to the bee organism under laboratory conditions are interpreted. The negative effects of honey buckwheat syrup as a solvent for “EM® PROBIOTIC FOR BEES” have been revealed, leading to hyperprotenemia, where an excessive amount of protein can act as a trigger for the development of ketosis. In turn, the decomposition product of this protein, ammonia, that causes high enzyme activity in the form of aspartate aminotransferase (SGOT), and gamma-glutamyl transpeptidase (GGT) in the blood of experimental insects which were fed probiotic diluted with buckwheat honey syrup. At the same time, concentrations of the preparation diluted with sugar syrup solution were determined to cause an increase in the content of globulins in the haemolymph of experimental insects, which have important immunological significance. There was a decrease in creatinine in the haemolymph of experimental insects which had been fed “EM® PROBIOTIC FOR BEES”, diluted with sugar syrup solution (5 % – 780 µmol/l; 2.5 % – 700.8 µmol/l; 1.25 % – 62.4 µmol/l). Note the fact of satisfactory operation of the malpighian vessels of the excretory system of experimental insects. Thus, “EM® PROBIOTIC FOR BEES” is a promising agent with a differential effect on the biochemical parameters of bee hemolymph in the studied concentrations.

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“…Recently, colistin-resistant strains have been isolated from wounds and hospital-resident patients, which is considered as the last line of defense against multidrug-resistant strains [10,11]. Hence, screening new naturally occurring candidates for fighting these microbes is seriously needed, as these natural resources represent rich reservoirs for new antimicrobials and bioactive metabolites [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Bioguided Isolation of Antibiofilm and Antibacterial Pregnane Glycosides from Caralluma quadrangula: Disarming Multidrug-Resistant Pathogens

et al. 2021

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Methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant Acinetobacter baumannii (MDRAB) present a serious challenge because of their capability to cause biofilm resistance to commonly used antibiotics producing chronic infections and hindering the process of wound healing. In the current study, we investigated the antibacterial activity of Caralluma quadrangula extracts (MeOH, and its fractions CH2Cl2 and n-butanol) against multidrug-resistant MRSA USA300 and A. baumannii AB5057. In vitro, the MeOH extract and both fractions of C. quadrangula significantly inhibited biofilm formation and disrupted previously established biofilm by MRSA and MDRAB at all the tested concentrations (0.625, 0.313, and 0.156 mg/mL). In vivo, C. quadrangula extracts successfully decreased bacterial loads in MRSA-infected skin lesions in mice. Four pregnane glycosides and one flavone glycoside were isolated from the bioactive n-butanol fraction. The isolated compounds (Rus A–E) were tested for their biofilm inhibition and biofilm detachment activities. The results revealed that Rus C was the most active compound (IC50 = 0.139 mmole), while Rus E was the least active (IC50 = 0.818 mmole). These results support the potential use of C. quadrangula extracts or their isolated compounds for hindering the biofilm attachment and the virulence of MRSA and MDRAB and their application as a topical antimicrobial preparation for MRSA skin infections.

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Probiotic capability of novel lactic acid bacteria isolated from worker honey bees gut microbiota

Cited by 22 publications

References 25 publications

Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

Harmlessness, biostimulating and antiviral action of probiotic “Apinormin” for honey bees

Effect of “EM® PROBIOTIC FOR BEES” on the biochemical parameters of bee haemolymph in an entomological cage experiment

Bioguided Isolation of Antibiofilm and Antibacterial Pregnane Glycosides from Caralluma quadrangula: Disarming Multidrug-Resistant Pathogens

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