Probiotic characteristics of Lactobacillus strains isolated from cheese and their antibacterial properties against gastrointestinal tract pathogens

Abstract

In the present study, four Lactobacillus strains from the cheese were analyzed for its probiotic potential against enteropathogenic bacteria. The probiotic properties of the selected strains were also analyzed and the selected bacterial strains showed high tolerance in bile salts and organic acid. The strain L. plantarum LP049 showed maximum survival rate (92 ± 4.2% and 93.3 ± 2%) after 3 h of treatment at 0.25% (w/v) bile salts and 0.25% (w/v) organic acid concentrations. The ability of the Lactobacillus strains to adhere to human epithelial cells (HT-29 cell lines) was evaluated and L. plantarum LP049 showed maximum adhesion property (19.2 ± 1.1%) than other tested strains. The Lactobacillus strains produced lactic acid at various concentrations. Compared with other strains, maximum level of lactic acid (3.1 g/L), hydrogen peroxide (4.31 mM) and bacteriocin (31 AU/mg) was detected in LB049. The inhibitory activity of culture supernatant against various bacterial pathogens was observed. The zone of inhibition ranged between 6 ± 2 mm and 23 ± 2 mm. The cell free extract showed activity against, Escherichia coli (ATCC 10536), Salmonella enteritidis (ATCC 13076), Shigella flexneri (ATCC 29903), and Enterococcus faecium (ATCC 8459). Consequently, L. plantarum LP049 may be considered as a potential candidate for the production of novel bioactive metabolites for therapeutic and bio-protective applications.

Effects of Lactobacillus plantarum on growth traits, slaughter performance, serum markers and intestinal bacterial community of Daheng broilers

“…It is widely known that probiotics will multiply rapidly after entering the intestine, compete with harmful bacteria for nutrition, and inhibit the growth of harmful microorganisms (Foltz et al, 2017; Zhang et al, 2020). In addition, probiotics can directly regulate intestinal digestion and absorption of nutrients, or improve intestinal digestion and absorption of nutrients by regulating digestive enzyme activities, so as to promote the growth of animals (Jager et al, 2018).…”

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

“…The production of bacteriocins by Lactobacillus and their antibacterial properties against many pathogenic bacteria have been widely explored [ 10 , 11 , 12 ]. Similarly, their ability to produce hydrogen peroxide and their impact on pathogen growth inhibition have also been described [ 13 , 14 ]. However, in many cases, the antimicrobial activity of Lactobacillus strains is associated with organic acid production.…”

In vitro investigation on lactic acid bacteria isolatedfrom Yak faeces for potential probiotics

“…Besides, they could also inhibit the development of pathogenic microorganisms, prevent or reduce the course of bacterial, viral and antibiotic diarrhea, eliminate or reduce the symptoms of lactose intolerance and so on (Jarocki et al, 2020). Probiotics are widely used in treatment of human and animal gastrointestinal disorders because of their biological properties of promoting intestinal peristalsis and maintaining intestinal homeostasis (Wang et al, 2018;Jarocki et al, 2020;Zhang et al, 2020). The European Society for Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN group) recommended the usage of probiotics as an adjunctive/ preventive treatment for different types of diarrhea in pediatrics (Dioso et al, 2020).…”