Probiotic Characterization of Indigenous Kocuria flava Y4 Strain Isolated from Dioscorea villosa Leaves

Barik, Adyasa; Patel, Gaurav D.; Sen, Sudip Kumar; Rajhans, Geetanjali; Nayak, Chirasmita; Raut, Sangeeta

doi:10.1007/s12602-021-09877-2

Cited by 5 publications

(3 citation statements)

References 59 publications

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“…The Kocuria flava Y4 strain isolated from Dioscorea villosa L. leaves and its characterization made in our previous study [ 14 ] has good acid and bile tolerance along with good hydrophobicity. This strain was maintained at 35 °C and stored at 4 °C on nutrient agar slants.…”

Section: Methodsmentioning

confidence: 99%

Fortification of orange juice with microencapsulated Kocuria flava Y4 towards a novel functional beverage: Biological and quality aspects

Barik

Pallavi

Sen³

et al. 2023

Heliyon

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Section: Methodsmentioning

confidence: 99%

Fortification of orange juice with microencapsulated Kocuria flava Y4 towards a novel functional beverage: Biological and quality aspects

Barik

Pallavi

Sen³

et al. 2023

Heliyon

Self Cite

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“…Hemolytic activity of the bacterial isolate strain NB2 bacterial culture was assessed using the blood agar plate (5% v/v) (Barik et al, 2021 ). Cellular toxicity of bacterial isolate strain NB2 was assessed against Caco-2 cell lines (Dowdell et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Human Intestinal Bacteria Cytobacillus oceanisediminis NB2 for Probiotic Potential

et al. 2022

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Systemic characterization of the human gut microbiota highlighted its vast therapeutic potential. Despite having enormous potential, the non-availability of their culture representatives created a bottleneck to understand the concept of microbiome-based therapeutics. The present study is aimed to isolate and evaluate the probiotic potential of a human gut isolate. Physiochemical, morphological, and phylogenetic characterization of a human gut isolate identifies it as a rod-shaped gram-negative microbe taxonomically affiliated with the Cytobacillus genus, having an optimal growth at 37°C in a partially alkaline environment (pH 8.0). This human gut isolate showed continuous growth in the presence of salts (up to 7% NaCl and 10% KCl), antibiotics, metals and metalloids [silver nitrate (up to 2 mM); lead acetate (up to 2 mM); sodium arsenate (up to 10 mM); potassium dichromate (up to 2 mM)], gastric and intestinal conditions, diverse temperature (25–50°C), and pH (5–9) conditions making it fit to survive in the highly variable gut environment. Genomic characterization identified the presence of gene clusters for diverse bio-catalytic activity, stress response, and antimicrobial activity, as well as it indicated the absence of pathogenic gene islands. A combination of functional features like anti-amylase, anti-lipase, glutenase, prolyl endopeptidase, lactase, bile salt hydrolase, cholesterol oxidase, and anti-pathogenic activity is indicative of its probiotic potential in various disorders. This was further substantiated by the CaCo-2 cell line assay confirming its cellular adherence and biosafety. Conclusively, human gut isolate possessed significant probiotic potential that can be used to promote animal and human health.

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“…Phylogenetic analysis of identified prolyl endopeptidase with the other members of the serine protease family (S9A, S9B, S9C, and S9D) indicates clustering of human gut prolyl endopeptidase sequences with the other members of the S9A serine family (Figure 4). Sequence alignment of the PEP proteins with characterized prolyl endopeptidase of Aeromonas punctata PEP (apPEP) and Myoxococcus xanthus PEP (mxPEP-zPP), indicates the presence of catalytically important residues (Trp582, Arg627, Asp 625, Ser541, and His660) numbering as per Aeromonas punctata PEP (Barik et al, 2021) (Supplementary Figure S6). The presence of conserved catalytic residues indicates that this protein could be involved in the gluten hydrolytic potential of Cellulomonas sp.…”

Section: Characterization Of Prolyl Endopeptidase Featuresmentioning

confidence: 99%

Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health

Yadav

Kumar²,

Maurya³

et al. 2023

Front. Cell. Infect. Microbiol.

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Cellulomonas sp. HM71, a human gut microbe possesses metabolic machinery to catabolize antigenic gluten, hence, holds promises as microbial therapy to treat gluten-derived celiac disease. However, its efficacy, safety, and survivability in the gastrointestinal ecosystem await functional elucidation. The current study is designed to characterize Cellulomonas sp. HM71 for its physiological, genomic, and probiotic properties. The morphological and physiological assessment indicates it as a coccus-shaped gram-positive bacterium growing optimally at 30°C in a neutral environment (pH 7.0). Cellulomonas sp. HM71 showed continuous growth even in stressful environments (salinity up to 3% NaCl and 6% KCl), variable temperature (25°C to 35°C) and pH (5-9), antibiotics, and gastric and intestinal conditions. The Cellulomonas sp. HM71 genome harbors diversified genetic machinery to modulate humongous metabolic potential for the host. This was substantiated by the hemolytic and CaCo-2 cell line assay which confirms its cellular adherence and biosafety. Notably, genome analysis did not identify any pathogenic islands. Probiotic characterization indicates its potential to overcome waterborne infections and digestion-related disorders. Cumulatively, Cellulomonas sp. HM71 can be considered a probiotic strain for improving human health because of the highlighted functions.

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Probiotic Characterization of Indigenous Kocuria flava Y4 Strain Isolated from Dioscorea villosa Leaves

Cited by 5 publications

References 59 publications

Fortification of orange juice with microencapsulated Kocuria flava Y4 towards a novel functional beverage: Biological and quality aspects

Fortification of orange juice with microencapsulated Kocuria flava Y4 towards a novel functional beverage: Biological and quality aspects

Isolation and Characterization of Human Intestinal Bacteria Cytobacillus oceanisediminis NB2 for Probiotic Potential

Characterization of Cellulomonas sp. HM71 as potential probiotic strain for human health

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