Probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum alleviates age-inflicted oxidative stress and improves expression of biomarkers of ageing in mice

Abstract

The potential benefiting effects of probiotic Dahi on age-inflicted accumulation of oxidation products, antioxidant enzymes and expression of biomarkers of ageing were evaluated in mice. Probiotic Dahi were prepared by co-culturing in buffalo milk (3% fat) Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC-86 and Lactococcus lactis ssp. lactis biovar diacetylactis NCDC-60) along with selected strain of Lactobacillus acidophilus LaVK2 (La-Dahi) or combined L. acidophilus and Bifidobacterium bifidum BbVK3 (LaBb-Dahi). Four groups of 12 months old mice (6 each) were fed for 4 months supplements (5 g/day) of buffalo milk (3% fat), Dahi, La-Dahi and LaBb-Dahi, respectively, with basal diet. The activities of catalase (CAT) and glutathione peroxidase (GPx) declined and the contents of oxidation products, thiobarbituric acid reactive substances (TBARS) and protein carbonyls, increased in red blood corpuscles (RBCs), liver, kidney and heart tissues and superoxide dismutase (SOD) activity increased in RBCs and hepatic tissues during ageing of mice. Feeding ageing mice with La-Dahi or LaBb-Dahi increased CAT activity in all the four tissues, and GPx activity in RBCs and hepatic tissue, and a significant decline in TBARS in plasma, kidney and hepatic tissues and protein carbonyls in plasma. Feeding mice with probiotic Dahi also reversed age related decline in expression of biomarkers of ageing, peroxisome proliferators activated receptor-α, senescence marker protein-30 (SMP-30) and klotho in hepatic and kidney tissues. The present study suggests that probiotic Dahi containing selected strains of bacteria can be used as a potential nutraceutical intervention to combat oxidative stress and molecular alterations associated with ageing.

RETRACTED ARTICLE: Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice

“…The liver is the major site of glutathione synthesis and transport and thus could explain the apparent effects of LR consumption on GPx activity in liver and RBCs. Similarly, consumption of probiotic cultured milk has also been shown to enhance activities of SOD and CAT in various clinical and experimental studies (Shih and Yen 2007;Yadav et al 2008;Zhou et al 2010;Kaushal and Kansal 2012) which could be attributed to release of antioxidative peptides from milk proteins by proteolytic activity of probiotic strains. Moreover, it has also been shown that probiotic supplementation may protect against oxidative stress by decreasing the accumulation of ROS (Kullisar et al 2002;Martarelli et al 2011) possibly by mechanisms involving metal ion chelation, scavenging of the oxidant compounds, or even inhibiting ROS production in the intestine (Azcárate-Peril et al 2011).…”

Preparation, characterization and in vitro antioxidative potential of synbiotic fermented dairy products

“…Hutt et al (2009) reported that consumption of a synbiotic product reduced the systemic oxidative stress due to colonization of Helicobacter pylori. Kaushal and Kansal (2012) reported that probiotic dahi containing L. acidophilus and B. bifidum reduces age-inflicted oxidative stress as studied by expression of biomarkers of ageing in mice. Moreover, results of revealed that synbiotic yogurt samples containing L. plantarum and L. fermentum, the DPPH radical inhibition was 85 and 82 %, respectively, at day 1 and the values increased as the storage time increased.…”

Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum improves phagocytic potential of macrophages in aged mice

“…The metabolites produced by the action of probiotics on milk constituents also possess immuno-stimulating potentials (Cavallo et al 1996). Recent studies showed that administration of Dahi prepared using these strains protects against enteric infection and gastrointestinal carcinogenesis Kansal 2008, 2009), and ameliorates age related oxidative stress and molecular alterations in mice (Kaushal and Kansal 2011). The present study was conducted to examine the effect of probiotic Dahi prepared using these strains on age related decline in macrophage phagocytic functions in mice.…”