Probiotic effect of Bacillus NL110 and Vibrio NE17 on the survival, growth performance and immune response of Macrobrachium rosenbergii (de Man)

Abstract

Eight hundred and eighty-¢ve strains of bacterial isolates from various samples associated with the natural habitat of Macrobrachium rosenbergii were screened for their probiotic potential. Two putative probionts namely Bacillus NL110 and Vibrio NE17 isolated from the larvae and egg samples, respectively, were selected for experimental studies and were introduced to the juveniles of M. rosenbergii (0.080 AE 0.001g) through di¡erent modes such as through feed, water and both. The probiotic potential of the above bacteria in terms of improvements in water quality, growth, survival, speci¢c growth rate (SGR), feed conversion ratio and immune parameters was evaluated. The treatment groups showed a signi¢cant improvement in SGR and weight gain (Po0.001). Survival among di¡erent treatment groups was better than that in the control group. There were also signi¢cant improvements in the water quality parameters such as the concentration of nitrate and ammonia in the treatment groups (Po0.05). Improvements in immune parameters such as the total haemocyte count (Po0.05), phenoloxidase activity and respiratory burst were also signi¢cant (Po0.001). It is concluded that screening of the natural micro£ora of cultured ¢sh and shell¢sh for putative probionts might yield probiotic strains of bacteria that could be utilized for an environment-friendly and organic mode of aquaculture.

Effects of dietary Bacillus pumilus on growth performance, innate immunity and digestive enzymes of giant freshwater prawns ( Macrobrachium rosenbergii )

“…However, the increased activity of amylase and protease in the dietary B. pumilus groups may have led to higher digestion and absorption of nutrients(Ziaei- Nejad et al, 2006), then the growth performance of M. rosenbergii may have been improved. Furthermore, studies in zebra fish, Danio rerio, demonstrated that some probiotics, for example Lactobacillus rhamnosus, could modulate the transcription of genes related to the differentiation and maturation of some tissues, thereby enhancing the growth of the animals(Avella et al, 2012;Gioacchini et al, 2011;Maradonna et al, 2013).The immune indices, including phagocytic activities and RBs activity, and the enzyme activity of PO, CAT, ACP and NOS were all significantly higher in all dietary B. pumilus groups than in the control group, and a higher survival rate was induced by dietary B. pumilus.Similar results have been observed in M. rosenbergii fed with other probiotics, such as Vibrio NE17(Rahiman, Jesmi, Thomas, & Hatha, 2010), Bacillus licheniformis(Kumar et al, 2013) and L. plantarum(Dash et al, 2016). A significant increase in immune indices caused by dietary B. pumilus was also reported in Nile tilapia (Oreochromis niloticus)(Srisapoome & Areechon, 2017) and striped catfish (Pangasianodon hypophthalmus)(Truong Thy et al, 2017).Hemocytes play a vital role in phagocytosis, nodule formation, encapsulation, hemocyte clumping, production of microbicidal compounds, mediation of cytotoxicity and so on(Johansson, Keyser, Sritunyalucksana, & Söderhäll, 2000;Smith, Brown, & Hauton, 2003), all of which have been proven to be an important part of the innate immunity of crustaceans.…”

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

“…There are several studies demonstrating the positive effects of probiotics Bacillus spp. on the growth of M. rosenbergii (Keysami et al., ; Kumar et al., ; Mujeeb Rahiman, Jesmi, Thomas, & Mohamed Hatha, ; Seenivasan, Radhakrishnan, Muralisankar, & Saravana Bhavan, ). For instance, Kumar et al.…”

“…There are several studies demonstrating the positive effects of probiotics Bacillus spp. on the growth of M. rosenbergii(Keysami et al, 2012;Kumar et al, 2013;Mujeeb Rahiman, Jesmi, Thomas, & Mohamed Hatha, 2010;Seenivasan, Radhakrishnan, Muralisankar, & Saravana Bhavan, 2016). For instance,Kumar et al (2013) showed that dietary Bacillus licheniformis addition at 1 × 10 9 cfu/g significantly improved the growth performance in M. rosenbergii juveniles after 60 days of feeding.…”

Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

“…Presence of E. coli in the gut of control prawn (10 -4 , ViBact*, Bini it TM and Biogen® incorporated diets [4][5][6][7][8][9][10][11][12][13][32][33][34][35][36][37][38][39][40][41][42]. Similarly, the dietary administration of Lactobacillus plantarum, Bacillus sp., and C. butyricum also produced a signi icant improvement in growth, carcase biochemical constituents, and activities of digestive enzymes in shrimps, Litopenaeus vannamei and Marsupenaeus japonicas [12,[43][44][45][46].…”