Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Kekkonen, Riina A.; Kajasto, E.; Miettinen, Minja; Veckman, Ville; Korpela, Riitta; Julkunen, Ilkka

doi:10.3748/wjg.14.1192

Cited by 99 publications

(95 citation statements)

References 43 publications

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“…Similar results have been reported from in vitro stimulation of human PBMCs with these LAB strains. 33,[142][143][144] These in vitro results are in keeping with the ability of these probiotic strains to induce regulatory mechanisms, rather than Th1 cytokine polarization, in vivo. Cytokine production in response to OVA restimulation was assessed in mesenteric lymph nodes…”

Section: Probiotics In Allergic Diseasesmentioning

confidence: 58%

“…30,31 Similar fi ndings have been reported in LAB-stimulated peripheral blood mononuclear cells (PBMCs). 32,33 This suggests that the intestinal microfl ora of the host may have a decisive infl uence on the response to treatment with a specifi c probiotic. Note, however, that this inhibitory effect of weak IL-12 inducers on the inducing capacity of strong inducers is DC-subset dependent, with inhibition seen in MDDCs, but not in blood myeloid DCs.…”

Section: Introductionmentioning

confidence: 99%

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Probiotics and immunity

et al. 2009

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Probiotics are defi ned as live microorganisms that, when administered in adequate amounts, confer a health benefi t on the host, including the gastrointestinal tract. While this benefi cial effect was originally thought to stem from improvements in the intestinal microbial balance, there is now substantial evidence that probiotics can also provide benefi ts by modulating immune functions. In animal models, probiotic supplementation is able to provide protection from spontaneous and chemically induced colitis by downregulating infl ammatory cytokines or inducing regulatory mechanisms in a strain-specifi c manner. In animal models of allergen sensitization and murine models of asthma and allergic rhinitis, orally administered probiotics can straindependently decrease allergen-specifi c IgE production, in part by modulating systemic cytokine production. Certain probiotics have been shown to decrease airway hyperresponsiveness and infl ammation by inducing regulatory mechanisms. Promising results have been obtained with probiotics in the treatment of human infl ammatory diseases of the intestine and in the prevention and treatment of atopic eczema in neonates and infants. However, the fi ndings are too variable to allow fi rm conclusions as to the effectiveness of specifi c probiotics in these conditions.

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Section: Probiotics In Allergic Diseasesmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Probiotics and immunity

et al. 2009

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“…Studies using IEC cultures have shown the downregulation of the secretion of the proinflammatory chemokine IL-8 (Frick et al 2007; Kamada et al 2008;Ma et al 2004;O'Hara et al 2006) and other NF-jB regulated chemokines, such as MCP-1 (Petrof et al 2009) or CCL-20 (Sibartie et al 2009), as well as a mechanism for the inhibition of IP-10 secretion. Also, a number of works demonstrated that probiotic bacteria directly interacting with immune cells elicit a differential cytokine production, thereby driving the polarization of T cells toward T h 1, T h 2, T h 17 or regulatory T cells (Foligne et al 2007a, b;Kekkonen et al 2008;Latvala et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

et al. 2012

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Significant health benefits have been demonstrated for certain probiotic strains through intervention studies; however, there is a shortage of experimental evidence relative to the mechanisms of action. Here, noninvasive experimental procedure based on a colon organ culture system has been used that, in contrast to most experimental in vitro models reported, can preserve natural immunohistochemical features of the human mucosa. This system has been used to test whether commensal lactobacilli (Lactobacillus paracasei BL23, Lactobacillus plantarum 299v and L. plantarum 299v (A -)) were able to hinder inflammation-like signals induced by phorbol 12-myristate 13-acetate (PMA)/ionomycin (IO). Whole genome microarrays have been applied to analyze expression differences, from which mRNA markers could be inferred to monitor the effect of putative probiotic strains under such conditions. Regarding the gene expression, PMA/IO treatment induced not only interleukin (IL)-2 and interferon gamma (IFN-c), as expected, but also other relevant genes related to immune response and inflammation, such as IL-17A, chemokine (C-X-C motif) ligand (CXCL) 9 and CXCL11. The ex vivo culturing did not modify the pattern of expression of those genes or others related to inflammation. Interestingly, this study demonstrated that lactobacilli downregulated those genes and triggered a global change of the transcriptional profile that indicated a clear homeostasis restoring effect and a decrease in signals produced by activated T cells.

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“…Selected species of these genera may, therefore, be of therapeutic benefit in gingivitis. However, the effects of these bacteria on the release of inflammatory mediators associated with gingival inflammation are likely strain specific, and some commercially available strains used as probiotics may have proinflammatory effects [11,12]. Indeed, a commercially available yoghurt containing Lactobacillus bulgaricus and Streptococcus thermophilus , with additional Lactobacillus casei DN 114 001, was shown to enhance LPS-stimulated production of proinflammatory cytokines, including TNF-α and IL-1β, in blood culture [12].…”

Section: Introductionmentioning

confidence: 99%

Ex vivo anti-inflammatory effects of probiotics for periodontal health

Schmitter

Fiebich²,

Fischer

et al. 2018

Journal of Oral Microbiology

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Background: Probiotic bacteria with anti-inflammatory properties have the potential to be of therapeutic benefit in gingivitis.Objective: To evaluate the effects of potential probiotic strains on inflammatory mediators involved in early gingivitis using an ex vivo inflammation model.Methods: Strains were screened in viable and attenuated forms for effects on bacterial lipopolysaccharide (LPS)-stimulated release of interleukins (IL)-1β, -6 and -8, tumor necrosis factor-α, prostaglandin E2 and 8-isoprostane from human primary monocytes, and then, if anti-inflammatory effects were shown, on IL-1β-stimulated release of inflammatory mediators from primary gingival fibroblasts. Lead strains were evaluated for optimal dosing, batch-to-batch variation and functional consistency in toothpaste.Results: Twenty-one of 73 strains showed anti-inflammatory effects in monocytes; of which, seven showed effects in both viable and attenuated forms. Seven of 14 strains showed effects in fibroblasts. Strains Lactobacillus paracasei LPc-G110(SYBIO-15) and Lactobacillus plantarum GOS42(SYBIO-41) induced statistically significant dose-dependent reductions in the release of multiple inflammatory mediators from monocytes, which were consistent across batches. Viable L. paracasei LPc-G110 tooth paste significantly reduced IL-6, IL-8 and prostaglandin E2 release from monocytes versus placebo.Conclusion: Strains L. paracasei LPc-G110 and L. plantarum GOS42 have potential for use as probiotics in oral care products to reduce gingival inflammation.

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Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Abstract: AIM:To investigate the capacity of potentially probiotic strains from six bacterial genera to induce cytokine production alone or in combinations in order to identify potential enhancing or synergistic effects in order to select probiotic bacteria for in vivo purposes. METHODS:

Cited by 99 publications

References 43 publications

Probiotics and immunity

Probiotics and immunity

Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa

Ex vivo anti-inflammatory effects of probiotics for periodontal health

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