Probiotic potency of Lactobacillus plantarum KX519413 and KX519414 isolated from honey bee gut

Abstract

The Indian honey bee Apis cerana indica, which harbors an abundant and diverse range of lactic acid bacteria (LAB) in their gut with beneficial effects, was used as the source for the isolation of LAB. In the present study, two LAB isolates from honey bee gut were selected primarily based on their phenotypic and selective biochemical characterization, followed by PCR and identified using 16S rRNA sequencing as Lactobacillus plantarum and were registered in National Centre for Biotechnology Information under accession number KX519413 and KX519414. The probiotic potency of test strains indicated their survivability at acidic pH, bile salts and viability in simulated gastric juice enabling them to withstand gastrointestinal tract conditions. Evaluation of cell surface properties suggested that they possess an important defense mechanism against the pathogen since they are hydrophobic, auto-aggregative and have co-aggregative ability. Further, efficient exopolysaccharide production by them indicates not only their ability to enrich biofilm formation and auto-aggregation, but also enhances bacterial adhesion and colonization on the host intestinal tract. The present study concluded that L. plantarum from the gut of Apis cerana indica possesses probiotic potency, and potential candidates for use as food besides application in nutraceutical and pharmaceutical industries.

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

“…The probiotic adherence ability on epithelial cells is important for health-promoting effects, which is required to maintain their intestinal colonization, besides facilitating the augmentation of intestinal mucosal barrier. Therefore, this will prevent cells from the adherence of pathogenic bacteria [31], thus increase the immunity of the organism. It was reported that the hydrophobicity and autoaggregation ability of the probiotic is associated with adherence ability, which is used as an indicator to evaluate bacterial adhesion ability [32].…”

Genome Analysis of Lactobacillus plantarum Isolated From Some Indian Fermented Foods for Bacteriocin Production and Probiotic Marker Genes

“…The percentage hydrophobicity for L. plantarum strains DHCU70 and DKP1 was 55.57% and 5.40% for xylene, respectively, and 42.9 and 40.88% for hexadecane, respectively (Supplementary Table S2c). L. plantarum shows a defense mechanism against the pathogen due to its hydrophobic and auto aggregative ability (Honey Chandran and Keerthi, 2018).…”

The Effect of Pesticides on the Microbiome of Animals

“…They also inhibited the growth of the main pathogen of the honey bee-Paenibacillus larvae, which means that the studied lactobacilli have healthy probiotic properties. Lactobacilli are able to survive at acidic pH values, bile salts, and show vitality in the gastric juice [106], which makes them a potential candidate for use in the nutraceutical and pharmaceutical industries. Tests with Lactobacillus bacteria also revealed their probiotic effect [107].…”