Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products

Abstract

Lactobacilli species are an effective biotherapeutic alternative against bacterial infections and intestinal inflammatory disorders. However, it is important to evaluate their beneficial properties, before considering them as probiotics for medical use. In this study we evaluated some probiotic properties of Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLSD, Lactobacillus helveticus IMAU70129, and Lactobacillus casei IMAU60214 previously isolated from dairy products and as control Lactobacillus casei Shirota. Experimental evaluations revealed that all strains expressed hydrophobicity (25–40%), auto-aggregation (55–60%), NaCl tolerance (1–4%), adhesion to Caco-2 cells (25–33%), partial inhibition on adherence of Escherichia coli ATCC 35218, Salmonella Typhimurium ATCC 14028, and Staphylococcus aureus ATCC 23219. Cell-free supernatants (CFS) of Lactobacilli also inhibit growth of these pathogens. In immunomodulatory properties a reduction of interleukin-8 (IL-8) and nitric oxide (NO) release was observed in assays with Caco-2 cells stimulated with interleukin-1β (1 ng/mL), or lipopolysaccharide (0.1 µg/mL). On the other hand, the damage induced to Caco-2 cells with sodium dodecyl sulfate (SDS) was attenuated when the cultured cells were pretreated with L. rhamnosus KLDS, L. helveticus IMAU70129 and L. casei IMAU60214. These Lactobacilli possess probiotic properties determined by both an antagonistic activity on pathogenic bacteria and reduction in the inflammatory response of cells treated with SDS, a pro-inflammatory stimulant.

Safety Assessment of One Lactiplantibacillus plantarum Isolated from the Traditional Chinese Fermented Vegetables—Jiangshui

“…Auto-aggregation and co-aggregation are some of the abilities of probiotics to persist in the intestinal tract and adhere to mucus and epithelial cells in the host, which are utilized for screening the potential probiotics [ 23 , 24 ]. As depicted in Figure 1 D, the novel isolates exhibited a superior auto-aggregation at 2 h and 3 h compared to the E. coli and S. aureus , and the rate of it was nearly half of the others at 2 h, which provided the prerequisite ability for the isolate to intestinal epithelial cells.…”

“…As depicted in Figure 1 D, the novel isolates exhibited a superior auto-aggregation at 2 h and 3 h compared to the E. coli and S. aureus , and the rate of it was nearly half of the others at 2 h, which provided the prerequisite ability for the isolate to intestinal epithelial cells. The observed results showed the ability of co-aggregation of the L. plantarum isolates to E. coli or S. aureus has dramatically increased after 1 h. In pathogenic bacteria, aggregation was usually associated with pathogenesis mechanisms, as the formation of microcolonies may lead to an effective increase in the concentration of effectors secreted in or near the host cell, thereby regulating toxicity [ 23 ]. Conversely, the aggregation ability of probiotics, which is needed for probiotics to survive in the host intestines, is also an effective protective mechanism, reducing greatly the colonization of pathogenic bacteria after its aggregation with pathogenic bacteria on the surface [ 25 ].…”

Probiotic characterization of Lactiplantibacillus plantarum HOM3204 and its restoration effect on antibiotic-induced dysbiosis in mice

“…In despite of its benefits, probiotic effects are strain-specific. The beneficial effects of probiotics relate to their physiological characteristics: gastrointestinal transit ability, intestinal epithelial cell adhesion ability and antimicrobial activity (Dunne et al 2001;Rocha-Ramirez et al 2021). Another important factor limiting the efficacy of probiotics is their survivability during processing and storage (Gonzalez-Ferrero et al 2018).…”

“…2001; Rocha‐Ramirez et al . 2021). Another important factor limiting the efficacy of probiotics is their survivability during processing and storage (Gonzalez‐Ferrero et al .…”

Determination of prophylactic and therapeutic effectiveness of probiotic strain Escherichia coli 39-SN

“…Accordingly, the ability to colonize the intestines in humans, animals, or birds is different. When selecting strains for probiotics, these and many other biological properties must be taken into account [ 4 , 5 ]. Subsequently, the approach of developing probiotics should be based on the study of many parameters, including a comprehensive assessment of the properties of microorganisms – probionts.…”