Probiotic strains alleviated OVA-induced food allergy in mice by regulating the gut microbiota and improving the level of indoleacrylic acid in fecal samples

Liu, Wenwei; Li, Qian; Fang, Zhifeng; Wang, Hongchao; Zhu, Jinlin; Lee, Yuan‐Kun; Zhao, Jianxin; Zhang, Hao; Zhao, Jianxin

doi:10.1039/d1fo03520g

Cited by 35 publications

(33 citation statements)

References 52 publications

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“…Interestingly, LTA increased the gut microbiota diversity and enhanced Th1 immune response in the present study. Furthermore, the gut microbiota and its short-chain fatty acid metabolites can alleviate OVA-A pathological symptoms by decreasing Th2 immune responses . LTA contributes to intestinal homeostasis by regulating short-chain fatty acid metabolism; therefore, the potential activity of LTA in regulating gut microbiota and intestinal immunity has attracted much attention .…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the gut microbiota and its short-chain fatty acid metabolites can alleviate OVA-A pathological symptoms by decreasing Th2 immune responses. 42 LTA contributes to intestinal homeostasis by regulating short-chain fatty acid metabolism; therefore, the potential activity of LTA in regulating gut microbiota and intestinal immunity has attracted much attention. 24 LTA intervention was found to increase the abundance of Clostridiales, Lachnospiraceae, Lactobacillus, Prevotella, and Ruminococcus.…”

Section: ■ Discussionmentioning

confidence: 99%

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L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Gong

et al. 2022

J. Agric. Food Chem.

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Ovalbumin (OVA), a common food protein, can cause deadly allergies with intestine-specific immune reactions. L-Theanine (LTA) shows great potential for regulating intestinal immunity. To investigate the regulatory effect of LTA intervention on intestine-specific immunity, a 41 day experiment was performed on BALB/c OVA-sensitized mice. The results show that injecting female mice intraperitoneally with 50 μg of OVA and administering 30 mg of OVA 4 times can successfully establish an OVAsensitized mouse model. LTA intervention significantly increased weight gain and thymus index (p < 0.05), decreased allergy and diarrhea scores (p < 0.05), and improved jejunum structure. Meanwhile, the histological score and degranulation of mast cells decreased. LTA intervention increased Clostridiales, Lachnospiraceae, Lactobacillus, Prevotella, and Ruminococcus abundance while decreasing Helicobacter abundance. Flow cytometry and Western blotting results indicated that 200 and 400 mg/kg of LTA upregulated the expression of T-bet and Foxp3 proteins (p < 0.05), thus promoting the differentiation of jejunum CD4+ T cells to Th1 and Tregs and increasing the cytokines IFN-γ, IL-10, and TGF-β (p < 0.05). We found that 200 and 400 mg/kg of LTA downregulated the expression of RORγt and GATA3, thus inhibiting the differentiation of Th2 and Th17 cells and decreasing cytokines IL-4, IL-5, IL-13 TNF-α, IL-6, and IL-17A (p < 0.05). LTA inhibited the degranulation of mast cells and significantly decreased the serum levels of OVA-IgE, HIS, and mouse MCPT-1 (p < 0.05). Therefore, LTA intervention alleviated OVA allergy by improving intestine-specific immunity.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Gong

et al. 2022

J. Agric. Food Chem.

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“…Although no effect on sensitizing markers was detected, signi cantly increased expressions of Foxp3, TGF-β, and IL-10 ileum genes were observed, as well as increased levels of the tryptophan metabolite kynurenine (19). Feeding Bi dobacterium longum to foodallergic mice reduced IL-17 levels (20).…”

Section: Discussionmentioning

confidence: 92%

Early-life Gut Microbiota in Food Allergic Children and Its Impact on The Development of Allergic Disease

Yan

Xiang

et al. 2023

Preprint

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Background: The prevalence of food allergy (FA) has been increasing steadily over the past 2 decades to 3 decades, with diversified symptoms and increasing severity. The long-term outcome will affect the growth and development of children, and it may be associated with inflammatory bowel disease. However, its pathogenesis remains unclear. Previous studies have shown that early infancy is a crucial time when gut microbiota has an impact on the development of food allergy. However, there is no unified perspective on the characteristics on dysbiosis in early life link between dysbiosis and the future of allergic diseases. Objective: We investigated the association between composition in gut microbiota in early life and long-term clinical manifestations in children with food allergy. Methods: In this study, 16S rDNA gene high-through sequencing method was used to analyze the composition of gut microbiota in children with food allergy in early life in southern Zhejiang, and the clinical manifestations were followed up for 2 years to analyze the influence of gut microbiota changes in early life on the later clinical manifestations. Results: we found that the diversity of gut microbiota in children with food allergy did not change, but the abundance of microbiota changed. The bacterial markers of food allergy may be Akkermansia, Parabacteroides, Blautia and Escherichia-Shigella, the lack of gut microbiota many be Bifidobacteriumand Clostridium. After two years，two of the ten children with food allergy still had symptoms of food allergy, the intestinal flora of these two cases in early childhood showed an increase in Escherichia-Shigella and a decrease in Bifidobacterium, The other eight cases had remission of allergic symptoms. Conclusion: We concluded that food allergy is not associated with intestinal flora diversity. Most of the symptoms of food allergy can be relieved by the age of 3 years old, which is related to the composition of early intestinal flora. Escherichia-Shigellaand Bifidobacterium may affect the prognosis of food allergy.

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“…Escherichia Shigella , belonging to the family Enterobacteriaceae, was one of the culprits of acute diarrhea in intestinal infection, which explained severe diarrhea symptoms in GS mice. It has been reported that Escherichia Shigella was significantly increased in OVA-sensitized mice …”

Section: Discussionmentioning

confidence: 99%

Combination of Gut Microbial Features and the Proteomic Pattern Revealed Changes in Specific Intestinal Luminal Factors and Mechanisms of Their Regulation of Gluten Allergy

Xie

Liu

et al. 2023

J. Agric. Food Chem.

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Recent research consensus has highlighted the role of intestinal luminal factors in the association between intestinal microenvironment homeostasis and food allergy. However, the association between intestinal immune homeostasis and food allergy-related proteomic features remains elusive. In this study, we aimed to investigate the changes in gluten allergy (GA)-defined phenotypes and endotypes and intestinal microenvironment factors in BALB/c mice and linked GA to colonic proteomic signatures. Combined with increased allergy and diarrhea scores, intense antibody responses and abnormalities in T-cell cytokine production were induced in mice. GA-associated disruption of intestinal microenvironment homeostasis was underlined by the increased colonic pH, decreased intestinal antioxidant capacity, impaired intestinal barrier function, and decreased production and imbalanced proportions of short-chain fatty acids. 16S rRNA amplicon sequencing showed that the gut microbiota dysbiosis in mice was characterized by significant enrichment of six bacterial taxonomic units, including Prevotellaceae, Escherichia Shigella, Alloprevotella, Escherichia coli, Bacteroides vulgatus, and Lachnospiraceae bacterium DW59, which was correlated with immune end points. Using a label-free proteomics quantitative approach, 24 differentially expressed proteins linking GA-induced gut dysbiosis were identified, with four of them enriched in the serine endopeptidase inhibitor activity pathway. The development of GA in mice was associated with changes in specific intestinal luminal factors and may be mediated by serine protease activity-associated metabolic routes.

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Probiotic strains alleviated OVA-induced food allergy in mice by regulating the gut microbiota and improving the level of indoleacrylic acid in fecal samples

Abstract: Food allergy (FA) is a common immune disorder caused by food antigens.

Cited by 35 publications

References 52 publications

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Early-life Gut Microbiota in Food Allergic Children and Its Impact on The Development of Allergic Disease

Combination of Gut Microbial Features and the Proteomic Pattern Revealed Changes in Specific Intestinal Luminal Factors and Mechanisms of Their Regulation of Gluten Allergy

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