Probiotic use of lactobacilli in the human female urogenital tract

McGroarty, Jacqueline A.

doi:10.1111/j.1574-695x.1993.tb00337.x

Cited by 114 publications

(45 citation statements)

References 106 publications

(111 reference statements)

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“…They [7,12]. Klebanoff et al [10] have studied the control of the vaginal microflora by H 2 O 2 -generating lactobacilli and expressed that these microorganisms contribute to nonspecific antimicrobial host defense, probably through interaction with peroxidase and a halide.…”

Section: Discussionmentioning

confidence: 99%

“…They exert a protective role mainly by a combination of steric exclusion and inhibitory substance production [12]. Among these substances, lactic acid produced from carbohydrates helps to maintain a low vaginal pH [11].…”

mentioning

confidence: 99%

“…The characteristics of lactobacilli for probiotic use in the human female urogenital tract have been described previously [12]. Our objective is the selection of Lactobacillus species for probiotic use in the human vagina as a barrier to infections and as an alternative to long-term use of antibiotics.…”

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confidence: 99%

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Selection of Vaginal H2O2-Generating Lactobacillus Species for Probiotic Use

Ocaña¹,

Holgado²,

Nader‐Macías³

1999

Curr Microbiol

112

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Lactobacilli are believed to contribute to the control of the vaginal microflora by different mechanisms such as production of antagonistic substances like lactic acid, bacteriocins, and H2O2. This paper describes the selection of H2O2-generating lactobacilli among 35 hydrophobic isolates from the human vagina. Lactobacillus crispatus F117, which generated the highest H2O2 level, was chosen to study: (a) the kinetics of H2O2 production considering different culture conditions, and (b) the effect of this metabolite on the growth of urogenital tract pathogens. The levels of H2O2 in L. crispatus supernatant increased during its growth and were maximum at the early stationary phase (3.29 mmol H2O2 L-1) under aerated conditions (agitated cultures). In nonagitated cultures there were no detectable levels of H2O2. L. crispatus F117 spent supernatant inhibited Staphylococcus aureus growth in plaque assay. Inhibition was due to H2O2 since catalase treatment of the supernatant suppressed inhibition. In mixed cultures performed with L. crispatus and S. aureus a significant decrease in pathogen growth was observed. The inhibitory effect depended on the initial inoculum of S. aureus. Further evaluation of the properties of L. crispatus F117 will be performed to consider its inclusion in a probiotic for local use in the vaginal tract.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Selection of Vaginal H2O2-Generating Lactobacillus Species for Probiotic Use

Ocaña¹,

Holgado²,

Nader‐Macías³

1999

Curr Microbiol

112

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“…There has been an increasing recognition of the role of lactobacilli in the maintenance of homeostasis within dynamic ecosystems such as the vagina, and in prevention of colonization and infection caused by pathogenic organisms (McGroarty, 1993). Reid et al (1988) showed that certain Lactobacillus strains undergo coaggregation with uropathogens and suggested that this phenomenon is an important factor in the establishment and maintenance of a healthy urogenital flora.…”

Section: Resultsmentioning

confidence: 99%

Factors affecting the coaggregation ability of vaginal Lactobacilli withCandida spp.

2009

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In this study, the capacity of 30 strains of lactobacilli to coaggregate with Candida albicans ATCC 10239, C. albicans AJD 180 and Candida krusei ATCC 6258 were studied in vitro. A marked coaggregation with C. albicans was observed for two strains of Lactobacillus crispatus, a strain of Lactobacillus cellobiosus and a strain of Lactobacillus salivarius. Coaggregation occurred at a pH range from 3 to 7, some strains showing optimal binding at high and other strains at low pH. Treatment of lactobacilli at 70 or 85 °C for 20 min or treatment of the bacteria with pepsin abolished their capacity of coaggregation. The results may be of importance when trying to establish probiotics for vaginal use.

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“…The epithelial cells of the intestine are covered by a protective layer of mucus, which is a complex mixture of glycoproteins and glycolipids, with large glycoprotein mucin being the main component. Complexes of glycoproteins in the mucus layer prevent the host from harmful antigens and pathogens, and promote luminal motility (McGroarty, 1993;Van Tassell and Miller, 2011). Therefore, adhesion to this mucus is required for probiotic interaction to occur with the host and to elicit a response (Van Tassell and Miller, 2011).…”

Section: Resultsmentioning

confidence: 99%

Detection and identifcation of adherence genes of intestinal-origin Lactobacillus and Pediococcus strains grown on gastric mucin in vitro

2017

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One of the primary selection criteria for potential probiotics is the ability to adhere to the host gastrointestinal tract. This study evaluated the in vitro adhesion ability on gastric mucin of two Lactobacillus casei strains (AP and AG) and two Pediococcus acidilactici strains (BE and BK), and identified the corresponding genes responsible for adherence. Adhesion assays were performed in 96-well polystyrene microtiter plates using gastric mucin from porcine stomach as the matrix. An in vitro study on gastric mucin revealed that lactobacilli had a greater adherence ability compared with pediococci strains. The potential adherence genes were investigated using polymerase chain reaction (PCR) technology. Using specific primers, PCR studies amplified 150 base pairs of a potential mub gene and 161 base pairs of a potential ef-Tu gene, but no amplified bands for potential map and bac genes were obtained. Sequence comparisons showed that the 150 and 161 amplified base pairs were respectively homologous to the mub of Pediococcus acidilactici and ef-Tu genes of Lactobacillus paracasei. We concluded that the adherence ability of two strains of Lactobacillus casei (AP and AG) and two strains of Pediococcus acidilactici (BE and BK) on gastric mucin is in accordance with the presence of ef-Tu and mub genes. High level attachment in lactobacilli is likely to correlate with the ef-Tu gene, which is a lactobacilli-specific adhesive gene.

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Probiotic use of lactobacilli in the human female urogenital tract

Cited by 114 publications

References 106 publications

Selection of Vaginal H2O2-Generating Lactobacillus Species for Probiotic Use

Selection of Vaginal H2O2-Generating Lactobacillus Species for Probiotic Use

Factors affecting the coaggregation ability of vaginal Lactobacilli withCandida spp.

Detection and identifcation of adherence genes of intestinal-origin Lactobacillus and Pediococcus strains grown on gastric mucin in vitro

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