Probleme bei der RNA-Markierung mit radioaktiven Vorstufen bei In-vivo-Versuchen

Dahnke, H.-G.

doi:10.1515/bchm2.1975.356.2.1555

Cited by 8 publications

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“…The failure to detect any zonal distribution of RNA incorporation (despite this being described by Rabes & Brandle, 1969;Fabrikant, 1968) must be due to the long incorporation time used in this study compared with the 1 hr used by the others, or to the use of different precursors, as it is reported that different labelled precursors can produce widely different results, depending on the length of incorporation and their rates of conversion to nucleotides (Muramatsu & Busch, 1965). It is reported that pyrimidine nucleotides can be incorporated into liver lipids, proteins and other compounds, especially if the time of incorporation is long (Dahnke, 1975;Schneider & Greco, 1971). As already mentioned, the small amount of incorporated activity that in this study was left after combined RNase-and DNase-treatment probably constitutes incorporation into sites other than nucleic acids.…”

Section: Discussionsupporting

confidence: 52%

AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

Lorup

1977

Cell Proliferation

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An autoradiographic study was made of the 3H‐uridine incorporation into RNA and DNA in nucleus and cytoplasm of parenchymal cells in the regenerating liver of the mouse after a pulse time of 2 hr. After a decreased uptake of precursor into the parenchymal nucleus during the first 6 hr compared with the normal value, incorporation increased and was maximal at 36 hr; normal values were restored at 72 hr. The cytoplasmic labelling, after an initial small decrease, reached a maximum at 12 hr; this changed to normal 48 hr after hepatectomy. RNase‐digestion of the liver sections left a small incorporation in both nucleus and cytoplasm: presumably DNA. This incorporation is maximal at 12 hr over the nucleus and at 24 hr over the cytoplasm. After a 2 hr pulse of 3H‐thymidine, there was a marked uptake of the precursor into DNA about 24 hr after hepatectomy. This was maximal at 48 hr and reached normal values at 72 hr. A small amount of incorporation of 3H‐thymidine into DNA was seen immediately after the operation, and this population of weakly labelled nuclei was still rather large 72 hr later.

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Section: Discussionsupporting

confidence: 52%

AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

Lorup

1977

Cell Proliferation

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“…We also collected fractions con [17][18][19][20] and attributable to high uridine catabolism in the liver [17,21,22]. As reported by Moyer et al [20] and Dahnke [23], [3H]cytidine was much better incorporated into soluble nucleotides and RNA. After 5 min, 20 % or more of the 3H label recovered in the liver extract was found to be incorporated into soluble nucleotides and RNA.…”

Section: Resultsmentioning

confidence: 99%

Pyrimidine metabolism and sugar nucleotide synthesis in rat liver

Rijcken

Hooghwinkel

Ferwerda

1990

Biochemical Journal

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With radioactive precursors, the labelling kinetics of the soluble pyrimidine nucleotides and of RNA were measured in rat liver to determine the contribution of the metabolic flows through synthesis de novo and the salvage pathway. To separate and quantify all pyrimidine nucleotides, an h.p.l.c. technique was developed using anion-exchange chromatography and reversed-phase chromatography. The concentrations of cytidine nucleotides were in the range of 30-45 nmol/g wet weight, and the concentrations of the uridine phosphates and of the UDP-sugars were approx. 6 and 20 times higher respectively. After a single injection of [14C]orotic acid and of [3H]cytidine, the specific radioactivities were determined as a function of time. The 1'C/3H ratio was calculated and gave a good indication of the involvement of the different flows. It could be concluded that UTP derived from synthesis de novo and from the salvage pathway is not completely mixed before being utilized. The flow of the salvage pathway is relatively more directed to RNA synthesis in the nucleus and that of synthesis de novo to cytoplasmic processes. For CTP it could also be concluded that the flow of the salvage pathway was relatively more directed to RNA synthesis in the nucleus. Because of the nuclear localization of the enzyme CMP-NeuAc (N-acetylneuraminate) synthase, special attention was paid to CMP-NeuAc. However, a conclusion about a location about the synthesis of CMP-NeuAc could not unequivocally be drawn, because of the small differences in 14C/3H ratio and the different values for the CDP-lipids.

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Characterization of RNA synthesis by isolated hepatocytes in suspension

Castle¹,

Kreamer²,

Liu³

et al. 1979

Archives of Biochemistry and Biophysics

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Probleme bei der RNA-Markierung mit radioaktiven Vorstufen bei In-vivo-Versuchen

Cited by 8 publications

References 0 publications

AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

Pyrimidine metabolism and sugar nucleotide synthesis in rat liver

Characterization of RNA synthesis by isolated hepatocytes in suspension

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Probleme bei der RNA-Markierung mit radioaktiven Vorstufen bei In-vivo-Versuchen

Cited by 8 publications

References 0 publications

AN AUTORADIOGRAPHIC STUDY OF THE 3H‐URIDINE AND 3H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

AN AUTORADIOGRAPHIC STUDY OF THE 3H‐URIDINE AND 3H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

Pyrimidine metabolism and sugar nucleotide synthesis in rat liver

Characterization of RNA synthesis by isolated hepatocytes in suspension

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AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER

AN AUTORADIOGRAPHIC STUDY OF THE ³H‐URIDINE AND ³H‐THYMIDINE INCORPORATION IN THE REGENERATING MOUSE LIVER