Mobile devices not only bring convenience to aspects of life but also change people’s behaviour in their daily lives, in particular reading. While most of the studies focus on reading books, there are few systematic publications primarily focusing on electronic periodicals, especially comparing the different needs of patrons from different faculties. Through an online questionnaire, the authors explored whether and how university patrons of the University of Hong Kong changed their reading behaviour of e-periodicals in the context of mobile devices. Six reading patterns were investigated: reading frequency, types of periodicals, preference of mobile devices, reading time spent, reading time slot and reading location. The authors purposefully compare subjects selected from three faculties (Education, Engineering and Science) to examine whether research and learning requirements affect their behaviours. The analysis found that reading patterns did indeed change after patrons adopted mobile devices to read periodicals. There are also some statistically significant differences among the faculties investigated, reflecting their different information needs. The findings can help academic libraries review their periodical subscription policies and reading promotion schemes to satisfy various patrons’ needs.