Problems Relating to the Biosynthesis of Peptide Antibiotics

Abraham, E. P.; Newton, G. G. F.; Warren, S. C.

doi:10.1016/b978-0-12-395524-1.50018-7

Cited by 11 publications

(7 citation statements)

References 74 publications

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“…Growing cultures also show a delay of synthesis of nisin since this did not start till the logarithmic phase of growth (Hirsch, 1951). I n this respect nisin synthesis resembles that of other polypeptide antibiotics (for review see Abraham, Newton & Warren, 1965) . This differential synthesis, if confirmed, could suggest that the basic peptides and nisin had a possible function in the life-cycle of the organism.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of the Antibiotic Nisin and Other Basic Peptides by Streptococcus lactis Grown in Batch Culture

Hurst

1966

Journal of General Microbiology

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SUMMARYStreptococcus Zactis was grown in batch culture in a complex organic medium. Growth of the culture was followed by estimating the extinction, the dry weight, protein, DNA and RNA of the organism; basic peptides were estimated by electrophoresis and nisin by bio-assay. By the end of the lag phase of growth when active DNA and RNA synthesis were already proceeding, the nisin which had been introduced with the inoculum could not be recovered, although the cocci contained other basic peptides. Rapid nisin synthesis started after an increase of about 5 0 % in the dry weight of the cocci had taken place. Initially, high molecular weight nisin was made, the concentration of which decreased as the incubation proceeded. This was followed by the production of lower molecular weight nisin which could be recovered 24 hr after the end of the incubation. Inocula of organisms derived a t intervals from the parent culture were tested in secondary cultures for length of lag of growth by re-inoculation into fresh medium of the same composition. During the lag phase of growth of the parent culture, the length of lag of growth of the secondary cultures fell to about half the original time. The shortest lag of the secondary cultures coincided with Commencement of logarithmic growth of the parent culture and with the disappearance of cellular nisin from the parent culture. The length of the lag of the secondary cultures returned to their original value as the parent culture reached stationary phase and coincided with maximum nisin concentration in the parent culture. It is suggested that the length of lag of growth is related to the presence of basic peptides.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of the Antibiotic Nisin and Other Basic Peptides by Streptococcus lactis Grown in Batch Culture

Hurst

1966

Journal of General Microbiology

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“…The data reported in this paper show that the thermal movement of the composite membrane is larger than that of the felt from which it was constructed but less than that reported for similar bitumens used in the construction of built-up roofs [7]. Further, the data show that the ap parent coefficient of linear thermal expansion ob tained in the across machine direction is appreci ably greater than that obtained for in the machine direction.…”

Section: Summary and Commentmentioning

confidence: 48%

“…The coefficient of cubical expansion of asphalt and coal-tar pitch was reported to be 3.5 to 3.9X10"4 per F over the temperature range of 32 to 140 F and 2.6X10"4 per F over the range of 200 to 300 F [7], respectively. If the assump tion is made that the linear expansion is about }i that of the cubical expansion, the linear ex pansion coefficient is 120X10"6 per F for asphalt and 90X10"6 per F for coal-tar pitch over the temperature ranges indicated.…”

Section: Thermal Movementmentioning

confidence: 99%

Effects of thermal shrinkage on built-up roofing

Cullen¹

1965

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The National Bureau of Standards is a principal focal point in the Federal Government for assur ing maximum application of the physical and engineering sciences to the advancement of technology in industry and commerce. Its responsibilities include development and maintenance of the national standards of measurement, and the provisions of means for making measurements consistent with those standards; determination of physical constants and properties of materials; development of methods for testing materials, mechanisms, and structures, and making such tests as may be neces sary, particularly for government agencies; cooperation in the establishment of standard practices for incorporation in codes and specifications; advisory service to government agencies on scientific and technical problems; invention and development of devices to serve special needs of the Government; assistance to industry, business, and consumers in the development and acceptance of commercial standards and simplified trade practice recommendations; administration of programs in cooperation with United States business groups and standards organizations for the development of international standards of practice; and maintenance of a clearinghouse for the collection and dissemination of scientific, technical, and engineering information. The scope of the Bureau's activities is suggested in the following listing of its four Institutes and their organizational units.

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“…The coefficient of cubical expansion of asphalt and coal-tar pitch is reported to be 3.5 to 3.9 X 10~4 (32°F to 140°F) and 2.6 X 1(T 4 (2Q0 S F to 300°F)/°F [7].…”

Section: Thermal Movementmentioning

confidence: 99%

“…7 These observations would certainly suggest that thermal shrinkage of the membrane may be involved in such failures .…”

mentioning

confidence: 99%

The effects of thermal shrinkage on built-up roofing

Cullen¹

1964

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Problems Relating to the Biosynthesis of Peptide Antibiotics

Cited by 11 publications

References 74 publications

Biosynthesis of the Antibiotic Nisin and Other Basic Peptides by Streptococcus lactis Grown in Batch Culture

Biosynthesis of the Antibiotic Nisin and Other Basic Peptides by Streptococcus lactis Grown in Batch Culture

Effects of thermal shrinkage on built-up roofing

The effects of thermal shrinkage on built-up roofing

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