Procedures for immunochemical study of histamine release from leukocytes with small volume of blood

May, Charles D.; Lyman, Margaret S.; Alberto, R.; Cheng, Jane

doi:10.1016/0021-8707(70)90056-0

Cited by 322 publications

(115 citation statements)

References 5 publications

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“…The tissue was removed from the samples and to the supernatants was added one tenth their volume of 70% PCA and precipitation of protein was carried out for 15 min. The samples were centrifuged at 800 gfor 10 min and the histamine content of the supernatant fraction was quantified by the spectrophotofluorometric method described by May, Lyman, Alberto & Cheng (1970). Separate experiments indicated that extraction of histamine from the supernatants did not significantly influence the results.…”

Section: Sensitization Proceduresmentioning

confidence: 99%

Antigen‐induced Bronchial Anaphylaxis in Actively Sensitized Guinea‐pigs: Effect of Long‐term Treatment With Sodium Cromoglycate and Aminophylline

Andersson

Bergstrand

1981

British J Pharmacology

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The effects of long‐term treatment with sodium cromoglycate (SCG) and aminophylline on antigen‐induced bronchoconstriction have been studied in guinea‐pigs actively sensitized according to two different regimens (one producing IgE‐ and IgG‐like antibodies and the other producing exclusively IgG‐like antibodies). Treatment for three weeks with SCG (10 mg/kg) and aminophylline (10,30 or 60 mg/kg) led to a decreased bronchial response capacity which persisted even three days after treatment ceased. In this respect SCG was effective only in guinea‐pigs sensitized to produce at least partly IgE‐like antibodies; aminophylline was effective in guinea‐pigs sensitized to produce both IgE and/or IgG antibodies. The results in vivo with SCG were reflected in vitro by a reduced capacity of chopped lung tissue to release histamine at antigen challenge; lungs from animals treated with aminophylline did not show reduced histamine releasing capacity. Acute treatment with atropine was shown to reduce significantly the antigen‐induced bronchial contraction in guinea‐pigs sensitized to produce both IgE‐ and IgG‐antibodies. No effect of atropine was seen on an IgG‐mediated anaphylaxis. Increased reactivity to methacholine but not to histamine was seen in guinea‐pigs sensitized to produce both IgG‐ and IgE‐antibodies. Long‐term treatment with SCG did not affect this hyperreactivity to methacholine. Decreased reactivity to isoprenaline was found in isolated tracheae taken from guinea‐pigs sensitized to produce both IgE‐ and IgG‐like antibodies compared to unsensitized guinea‐pigs. Long‐term treatment with SCG, but not with aminophylline, reversed this decreased reactivity.

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Section: Sensitization Proceduresmentioning

confidence: 99%

Antigen‐induced Bronchial Anaphylaxis in Actively Sensitized Guinea‐pigs: Effect of Long‐term Treatment With Sodium Cromoglycate and Aminophylline

Andersson

Bergstrand

1981

British J Pharmacology

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“…Mes was determined according to the method of May ef al., 16) while LT was determined by the partially modified method of Asciak et al 17) To 10 6 isolated MCs was added a sufficient amount of an L-cysteine solution to make its content 0.5% vivo The resulting mixture was pre incubated for 2 minutes, before a sufficient amount of a calcium ionophore A23187 ethanol solution was added to make its content 0.5°;() v/v. The mixture was shaken and incubated for another 10 minutes.…”

Section: Measurement Qf Histamine and Lt In Mes The Histamine Contenmentioning

confidence: 99%

Effect of Dietary n-3/n-6 Fatty Acid Ratio on the Total Count, Fatty Acid Composition, and Histamine and Leukotriene Concentrations of Mast Cells in Tunica Mucosa Bronchiorum of Type I Allergic Guinea Pig

Kuwamori

Wada

Takita

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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“…Ten mi\li)itres of blood were collected on \ ml EDTA 125 mM, pH 7 4. and 2-2 ml giucose-dextran (May et al, 1970). The blood suspension sedimented for 40 min, which allowed the separation of the leucocytes from the bulk of the red blood cells.…”

Section: Shr From Human Leucocytesmentioning

confidence: 99%

Seasonal increase of spontaneous histamine release in washed leucocytes from rhinitis patients sensitive to grass pollen

Weyer

Guinnepain²,

Sutra³

et al. 1990

Clinical and Experimental Immunology

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SUMMARYThe spontaneous histamine release (SHR) in basophils from patients sensitive lo grass pollen has been studied before and during the 1987 grass pollen season. Nineteen patients were recruited on seasonal rhinitis symptoms, positivity for cutaneous lests and for serum-specific IgE wilh grass pollen. At the time of the biological investigations the patients were following a clinical trial of hyposensitization, including placebo, calcium phosphate and aluminium hydroxide-adsorbed grass pollen extract treatments. During the pollen season, grass pollen counts and clinical scores were checked over a 4()~day period. Mean SHR was significantly higher during the pollen period than before, for the whole population of 19 patients (10'9% and 4-6%; P < 0-005) as well as when the high SHR responders were excluded (5-5% and 3-

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Procedures for immunochemical study of histamine release from leukocytes with small volume of blood

Cited by 322 publications

References 5 publications

Antigen‐induced Bronchial Anaphylaxis in Actively Sensitized Guinea‐pigs: Effect of Long‐term Treatment With Sodium Cromoglycate and Aminophylline

Antigen‐induced Bronchial Anaphylaxis in Actively Sensitized Guinea‐pigs: Effect of Long‐term Treatment With Sodium Cromoglycate and Aminophylline

Effect of Dietary n-3/n-6 Fatty Acid Ratio on the Total Count, Fatty Acid Composition, and Histamine and Leukotriene Concentrations of Mast Cells in Tunica Mucosa Bronchiorum of Type I Allergic Guinea Pig

Seasonal increase of spontaneous histamine release in washed leucocytes from rhinitis patients sensitive to grass pollen

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