Proceedings: Superovulation in the cow following treatment with PMSG and prostaglandin F 2alpha

Rp, Elsden; Lewis, Shirley; Cumming, I. A.; Ra, Lawson

doi:10.1530/jrf.0.0360455

Cited by 21 publications

(4 citation statements)

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“…Because of the variability in oestrous cycle length in the cow, however, treatment of this type causes a variable onset of oestrus and ovarian response (Scanlon et al, 1968). The administration of PMSG during the luteal phase, followed by prostaglandin treatment to induce rapid luteolysis gives a consistent oestrous onset and a high ovulatory response (Elsden, Lewis, Cumming & Lawson, 1974), the latter being higher when PMSG is given between Days 8 and 14 of the cycle (Phillipo & Rowson, 1975).…”

mentioning

confidence: 99%

The effect of cycle stage and plasma progesterone level on the induction of multiple ovulations in heifers

Sreenan¹,

Gosling²

1977

Reproduction

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mentioning

confidence: 99%

The effect of cycle stage and plasma progesterone level on the induction of multiple ovulations in heifers

Sreenan¹,

Gosling²

1977

Reproduction

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“…Elsden et al 1974;Smith 1974;Roche 1974; King and Robertson 1974;Lauderdale 1975;Louis et al 1915;Moore 1975; Hafs and Manns 1975; Motlik et al 1976) and by certain of its synthetic analogues (Tervit et al 1973;Cooper and Rowson 1975;Dobson et al 1975; Leaver et al 1915;Deletang 1915) had been recently observed in estrus (day 0) and all prostaglandin (PG) treatments were carried out between days 6 and l5 ofthe cycle when the corpus luteum (CL) is known to be maximally responsive to PG-induced luteolysis (Rowson et al1912 For personal use only. (Welch et al 1975) or the rectal wall (Motlik et al 1976 (Fig.…”

Section: Amentioning

confidence: 99%

Synchronization of Estrus and Ovulation in Cattle With the Prostaglandin Analogue Ay 24655

Betteridge¹,

Sugden²,

Eaglesome³

1977

Can. J. Anim. Sci.

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“…From the early 1940s until the 1970s, embryo transfer in cattle was carried out by using pregnant mare serum gonadotropin (PMSG) and follicle-stimulating hormone (FSH) [ 1 ]. From the 1970s onwards, the use of commercial pituitary extracts and prostaglandins was developed, and later, in the 1980s and 1990s, partially purified pituitary extracts and progesterone devices established commercial embryo transfer (ET) activities [ 2 , 3 , 4 , 5 ]. Although ovum pick up (OPU) and IVP are currently practised on a large scale, superovulation techniques have been the main tools for embryo production in cattle for decades, with uterine lavage collection being a very effective technique for embryo production, with an increased viability and cryotolerance [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Extra-Long-Acting Recombinant Bovine FSH (bscrFSH) on Cattle Superovulation

Gutiérrez-Reinoso

Aguilera

Navarrete

et al. 2022

Animals

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Over the last few years, several commercial FSH products have been developed for cattle superovulation (SOV) purposes in Multiple Ovulation and Embryo Transfer (MOET) programs. The SOV response is highly variable among individuals and remains one of the main limiting factors in obtaining a profitable number of transferable embryos. In this study, follicle stimulating hormone (FSH) from different origins was included in two SOV protocols, (a) FSH from purified pig pituitary extract (NIH-FSH-p; two doses/day, 12 h apart, four consecutive days); and (b) extra-long-acting bovine recombinant FSH (bscrFSH; a single dose/day, four consecutive days), to test the effects of bscrFSH on the ovarian response, hormone profile levels, in vivo embryo production and the pluripotency gene expression of the obtained embryos. A total of 68 healthy primiparous red Angus cows (Bos taurus) were randomly distributed into two experimental groups (n = 34 each). Blood sample collection for progesterone (P4) and cortisol (C) level determination was performed together with ultrasonographic assessment for ovarian size, follicles (FL) and corpora lutea (CL) quantification in each SOV protocol (Day 0, 4, 8, and 15). Moreover, FSH profiles were monitorised throughout both protocols (Day 0, 4, 5, 6, 7, 8, 9, 10, and 15). In vivo embryo quantity and quality (total structures, morulae, blastocysts, viable, degenerated and blocked embryos) were recorded in each SOV protocol. Finally, embryo quality in both protocols was assessed by the analysis of the expression level of crucial genes for early embryo development (OCT4, IFNt, CDX2, BCL2, and BAX). P4 and cortisol concentration peaks in both SOV protocols were obtained on Day 15 and Day 8, respectively, which were statistically different compared to the other time-points (p < 0.05). Ovarian dimensions increased from Day 0 to Day 15 irrespective of the SOV protocol considered (p < 0.05). Significant changes in CL number were observed over time till Day 15 irrespective of the SOV protocol applied (p < 0.05), being non- significantly different between SOV protocols within each time-point (p > 0.05). The number of CL was higher on Day 15 in the bscrFSH group compared to the NIH-FSH-p group (p < 0.05). The number of embryonic structures recovered was higher in the bscrFSH group (p = 0.025), probably as a result of a tendency towards a greater number of follicles developed compared to the NIH-FSH-p group. IFNt and BAX were overexpressed in embryos from the bscrFSH group (p < 0.05), with a fold change of 16 and 1.3, respectively. However, no statistical differences were detected regarding the OCT4, CDX2, BCL2, and BCL2/BAX expression ratio (p > 0.05). In conclusion, including bscrFSH in SOV protocols could be an important alternative by reducing the number of applications and offering an improved ovarian response together with better embryo quality and superior performance in embryo production compared to NIH-FSH-p SOV protocols.

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Proceedings: Superovulation in the cow following treatment with PMSG and prostaglandin F 2alpha

Cited by 21 publications

References 0 publications

The effect of cycle stage and plasma progesterone level on the induction of multiple ovulations in heifers

The effect of cycle stage and plasma progesterone level on the induction of multiple ovulations in heifers

Synchronization of Estrus and Ovulation in Cattle With the Prostaglandin Analogue Ay 24655

Effects of Extra-Long-Acting Recombinant Bovine FSH (bscrFSH) on Cattle Superovulation

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