Process conditions optimization for CoQ10 production by Paracoccus Species

Rambir,; Dubey, Kashyap Kr.; Bhansali, Anil G.; Behera, Binati

doi:10.1016/j.jbiotec.2008.07.642

Cited by 1 publication

(1 citation statement)

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“…Although an interest in CoQ 10 has been recently renewed due to the growing demands of the pharmaceutical industry [17], a few scale-up studies of the CoQ 10 fermentation have been conducted using microorganisms other than photosynthetic bacteria: optimization of culture conditions and scale-up to pilot and plant scales using A. tumefaciens [11] and statistical optimization of culture conditions and operation parameters in a 150-l fermenter using Paracoccus denitrificans [28]. As a result, a pilotscale fermentation of R. sphaeroides is necessary to verify its potential for commercial CoQ 10 production.…”

Section: Introductionmentioning

confidence: 99%

Coenzyme Q10 production in a 150-l reactor by a mutant strain of Rhodobacter sphaeroides

Kien

Kong

Lee

et al. 2010

J Ind Microbiol Biotechnol

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For the commercial production of CoQ(10), batch-type fermentations were attempted in a 150-l fermenter using a mutant strain of R. sphaeroides. Optimum temperature and initial aeration rate were found to be 30 degrees C and 2 vvm, respectively. Under optimum fermentation conditions, the maximum value of specific CoQ(10) content was achieved reproducibly as 6.34 mg/g DCW after 24 h, with 3.02 g/l of DCW. During the fermentation, aeration shift (from the adequate aeration at the early growth phase to the limited aeration in active cellular metabolism) was a key factor in CoQ(10) production for scale-up. A higher value of the specific CoQ(10) content (8.12 mg/g DCW) was achieved in fed-batch fermentation and comparable to those produced by the pilot-scale fed-batch fermentations of A. tumefaciens, which indicated that the mutant strain of R. sphaeroides used in this study was a potential high CoQ(10) producer. This is the first detailed study to demonstrate a pilot-scale production of CoQ(10) using a mutant strain of R. sphaeroides.

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Section: Introductionmentioning

confidence: 99%