Process control in cell culture technology using dielectric spectroscopy

Justice, C.; Brix, Alexander; Freimark, Denise; Kraume, Matthias; Pfromm, Peter H.; Eichenmueller, B.; Czermak, Peter

doi:10.1016/j.biotechadv.2011.03.002

Cited by 107 publications

(87 citation statements)

References 94 publications

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“…Typically online, these data are collected by indirect inline measurement, e.g. by estimating the cell number based on the oxygen demand or glucose consumption rate of adherent cells growing in semi-dynamic cultivation systems [58,84,85]. In a fixed-bed bioreactor, this is realized by the simultaneous measurement of oxygen concentrations at the inlet and outlet.…”

Section: Process Controlmentioning

confidence: 99%

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Grein¹,

Weidner²,

Czermak³

2017

New Insights Into Cell Culture Technology

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The industrial-scale manufacturing of viruses or virus-like particles in cell culture is necessary for gene therapy and the treatment of cancer with oncolytic viruses. Complex multistep processes are required in both cases, but the low virus titers in batch cultures and the temperature sensitivity of the virus particles limit the production scale. To meet commercial and regulatory requirements, each process must be scalable and reproducible and must yield high virus titers. These requirements are met by establishing a cell culture process that matches the properties of the virus/host-cell system and by using serum-free cell culture medium. This chapter focuses on two case studies to consider the different aspects of process design, such as the reactor configuration and operational mode: the continuous production of retroviral pseudotype vectors in a retroviral packaging cell line and the production of oncolytic measles virus vectors for cancer therapy.

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Section: Process Controlmentioning

confidence: 99%

Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

Grein¹,

Weidner²,

Czermak³

2017

New Insights Into Cell Culture Technology

View full text Add to dashboard Cite

show abstract

“…Insect viruses are central components of current manufacturing processes producing recombinant proteins such as enzymes (Vicente et al, 2010;Vicente et al, 2009) and human or animal vaccines (Justice et al, 2011) at industrial scale (virus cultures in the order of several m 3 /day, Boehringer Ingelheim Vetmedica). For decades large scale applications of biopesticides (in the order of 10 4 km 2 /year (Moscardi, 1999)) have made use of insect viruses targeting specifically their hosts (Mishra, 1998;Moscardi, 1999;Oliveira et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Purification of a recombinant baculovirus of Autographa californica M nucleopolyhedrovirus by ion exchange membrane chromatography

Grein

Michalsky

López

et al. 2012

Journal of Virological Methods

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“…However, very high volume fractions of non-biomass materials close to the sensor may influence the capacitance signal since the polarizable cells are replaced by non-polarizable materials [102]. These applications have already been reviewed by other authors [113,107]. …”

Section: Dielectric Spectroscopymentioning

confidence: 99%