Process Optimization of Nuciferine with Cellulase Auxiliary Ultrasonic-Assisted Extraction from Leaves of Nelumbo Nucifera Using Response Surface Methodology

Abstract

The cellulase auxiliary ultrasonic-assisted extraction process of nuciferine from Nelumbo nucifera leaves was optimized by response surface methodology. The extraction content of nuciferine was measured by HPLC, On the basis of single factor experiment, ethanol concentration, liquid-solid ratio, and extraction times were selected as independent variables, and the extraction content of nuciferine was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimum extraction conditions were 10% cellulase, 87% ethanol concentration, 1:26g/mL solid-liquid ratio, 3 times and 25 minutes. The content of nuciferine was 0.343mg/g. The extraction rate of nuciferine was 0.034% after cellulase was added, the extraction rate of nuciferine without cellulase was 0.010%. The extraction process obtained was reasonable, this experiment can provide a more excellent and reliable method for extracting nuciferine from leaves of Nelumbo nucifera.

A review on Extraction of alkaloid from Nelumbo nucifera Embryos and Leaves for Production of Dietary Supplement

“…The sample was soaked in 0.3M HCl-Methanol solvent, 1:1, v/v, and sonicated in ambient temperature for 30 minutes. Combining enzyme-assisted extraction with UAE also improvesd performance [16]. Lu et al used respond surface methodology to find optimal extraction conditions, and the results showed that 10% cellulose, 87% ethanol concentration, 1:26 g/ml solid-liquid ratio, 3 times and 25 minutes were most effective [16].…”

“…Combining enzyme-assisted extraction with UAE also improvesd performance [16]. Lu et al used respond surface methodology to find optimal extraction conditions, and the results showed that 10% cellulose, 87% ethanol concentration, 1:26 g/ml solid-liquid ratio, 3 times and 25 minutes were most effective [16]. Supercritical fluid extraction (SFE) uses the poor solubility of S-CO 2 to extract non polar compound, but the addition of methanol or ethanol can improve the solubility of S-CO 2 in polar compounds, which helps S-CO 2 extract polar compounds [13].…”

Valorization of Lotus Leaf by In Situ Expanded‐Bed Adsorption and Ultrasonic‐Assisted Desorption

“…and two‐phase solvents (petroleum ether/ammonia solution 9). Various intensification processes, such as microwave‐assisted heating 10, ultrasonic‐assisted extraction 11, enzyme‐assisted extraction 12, β ‐cyclodextrin‐assisted extraction 13, have been employed to improve the extraction rate. Although the research of this aspect has certain progress at present, further research still should be intended to reduce extraction efforts in order to make the operation simpler and more cost‐effective.…”