Processing cord blood from premature infants into autologous red‐blood‐cell products for transfusion

Khodabux, C. M.; Beckhoven, J. M. van; Scharenberg, J. G. M.; Barjiji, F. El; Slot, Manon C.; Brand, Anneke

doi:10.1111/j.1423-0410.2010.01440.x

Cited by 20 publications

(22 citation statements)

References 26 publications

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“…Cord blood units without clots or signs of hemolysis, when fractionated, have red cells with acceptable biochemical and functional properties during storage [13,16]. Accordingly, hematocrit gain after transfusion and time intervals between transfusions were similar in UCB-RBCs and A-RBCs.…”

Section: Discussionmentioning

confidence: 99%

“…Among these, umbilical cord blood (UCB) collection and processing to obtain RBC products for autologous transfusion have been extensively investigated [7,8,9,10,11,12]. The refinement of techniques of collection, processing and storage of UCB allowed the production of UCB-RBC units suitable for clinical use [13,14]. However, the small volume of UCB collected does not allow an adequate coverage of transfusion needs of preterm infants and this practice has nowadays been abandoned [12].…”

Section: Introductionmentioning

confidence: 99%

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Allogeneic Umbilical Cord Blood Red Cell Concentrates: An Innovative Blood Product for Transfusion Therapy of Preterm Infants

Bianchi¹,

Giannantonio

Spartano³

et al. 2014

Neonatology

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Background: Preterm infants often receive blood transfusions early in life. In this setting, umbilical cord blood (UCB) might be safer than adult blood (A) with respect to infectious and immunologic threats. Objectives: To evaluate, as a first objective, the feasibility of fulfilling transfusion needs of preterm infants with allogeneic UCB red blood cell (RBC) concentrates and, as a secondary objective, to assess the safety of allogeneic cord blood transfusions. Methods: At the Neonatal Intensive Care Unit and the UNICATT Cord Blood Bank of ‘A. Gemelli' Hospital in Rome, a prospective study was carried out over a 1-year period, enrolling newborns with gestational age ≤30 weeks and/or birth weight ≤1,500 g requiring RBC transfusions within the first 28 days of life. At first transfusion, patients were assigned to receive UCB-RBCs or A-RBCs depending on the availability of ABO-Rh(D)-matched UCB-RBC units. The same regimen (UCB-RBC or A-RBC units) was thereafter maintained, unless ABO-Rh(D)-matched UCB-RBC units were not available. Results: Overall, 23 UCB-RBC units were transfused to 9 patients; the requests for UCB-RBC units were met in 45% of patients at the first transfusion and in 78% at the subsequent transfusions. At a median follow-up of 57 days (range 6-219), no acute or delayed transfusion-related adverse events occurred. Hematocrit gain after transfusion and time intervals between transfusions were similar in the UCB-RBC and A-RBC group, as well. Conclusions: Transfusing allogeneic UCB-RBC units in preterm infants appears a feasible and safe approach, although the transfusion needs of our study population were not completely covered. More data are necessary to validate this novel transfusion practice. © 2014 S. Karger AG, Basel

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allogeneic Umbilical Cord Blood Red Cell Concentrates: An Innovative Blood Product for Transfusion Therapy of Preterm Infants

Bianchi¹,

Giannantonio

Spartano³

et al. 2014

Neonatology

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“…We previously showed that autologous RBCs could be harvested from CB collected after premature birth and, if successful, could cover up to 58% of the transfusion needs . These premature CB‐derived RBC transfusion products had a shorter shelf life (maximally 21 days) compared to adult RBCs, which can be stored for 35 days . To obtain RBCs, the premature CB was buffy coat depleted by centrifugation and the removed buffy coats contained approximately 40% of the white blood cells (WBCs).…”

mentioning

confidence: 99%

“…We aimed to set up a simple one‐step culture protocol using only human proteins and recombinant human cytokines in the culture medium. In view of the practical transfusion needs of premature infants, we focused on the cultured cells that could be obtained within 21 days of expansion, which could theoretically supplement autologous CB RBCs, which have a shelf life up to 21 days . To test the efficiency and efficacy of this culture protocol, we compared both premature and full‐term CB and cells derived from adult blood and bone marrow (BM).…”

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confidence: 99%

Exploring the use of expanded erythroid cells for autologous transfusion for anemia of prematurity

et al. 2013

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“…In contrast, cord RBCs deteriorate much faster under the same conditions and cannot be stored for more than 14 days without significant decrease in quality [28, 37]. Cryopreservation and subsequent storage at ultra-low temperatures may preserve cord RBCs and maintain a high quality of cord RBCs for use in intrauterine and neonatal transfusions.…”

Section: Introductionmentioning

confidence: 99%

Quality of Red Blood Cells Isolated from Umbilical Cord Blood Stored at Room Temperature

Zhurova

Akabutu²,

Acker

2012

Journal of Blood Transfusion

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Red blood cells (RBCs) from cord blood contain fetal hemoglobin that is predominant in newborns and, therefore, may be more appropriate for neonatal transfusions than currently transfused adult RBCs. Post-collection, cord blood can be stored at room temperature for several days before it is processed for stem cells isolation, with little known about how these conditions affect currently discarded RBCs. The present study examined the effect of the duration cord blood spent at room temperature and other cord blood characteristics on cord RBC quality. RBCs were tested immediately after their isolation from cord blood using a broad panel of quality assays. No significant decrease in cord RBC quality was observed during the first 65 hours of storage at room temperature. The ratio of cord blood to anticoagulant was associated with RBC quality and needs to be optimized in future. This knowledge will assist in future development of cord RBC transfusion product.

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Processing cord blood from premature infants into autologous red‐blood‐cell products for transfusion

Abstract: UCB can be processed into autologous products for premature infants. Shelf-life is limited to 14-21 days and compares unfavourably to stored whole blood. Considering the early transfusion needs in these infants, a short shelf-life would not be a practical objection.

Cited by 20 publications

References 26 publications

Allogeneic Umbilical Cord Blood Red Cell Concentrates: An Innovative Blood Product for Transfusion Therapy of Preterm Infants

Allogeneic Umbilical Cord Blood Red Cell Concentrates: An Innovative Blood Product for Transfusion Therapy of Preterm Infants

Exploring the use of expanded erythroid cells for autologous transfusion for anemia of prematurity

Quality of Red Blood Cells Isolated from Umbilical Cord Blood Stored at Room Temperature

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