Procyanidin B2 3″-O-gallate Isolated from Reynoutria elliptica Prevents Glutamate-Induced HT22 Cell Death by Blocking the Accumulation of Intracellular Reactive Oxygen Species

Song, Ji Hoon; Kim, Sil; Yu, Jae Sik; Park, Do Hwi; Kim, Song-Yi; Kang, Ki Sung; Lee, Sullim; Kim, Ki‐Hyun

doi:10.3390/biom9090412

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…3.1.9. Reynoutria elliptica Song et al [56] evaluated the neuroprotective effects of procyanidin isolated from Reynoutria elliptica seeds in a glutamate-induced cell death model in the murine hippocampal HT22 cell line. The seed extracts showed a significant antioxidant response as a result of glutamate-induced cell death.…”

Section: Hibiscus Sabdariffamentioning

confidence: 99%

Neuroprotective Potential of Seed Extracts: Review of In Vitro and In Vivo Studies

et al. 2023

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Introduction: Neurodegenerative diseases are characterized by neuronal dysfunction and death. Studies suggest that some seed extracts have a neuroprotective effect. Considering the increased incidence of these diseases and the need for new effective therapies with fewer side effects, this review aimed to assess the evidence of the efficacy and safety of seed extracts in experimental models of neurodegeneration. Material and Method: The search was carried out through studies published between 2000 and 2021 in Science Direct, PubMed, Scientific Electronic Library Online (SciELO), and Latin American Literature in Health Sciences (LILACS) databases, in which the effects of seed extracts in in vitro and in vivo experimental models of neurodegeneration were investigated. Based on the eligibility criteria, 47 studies were selected for this review. Results: In the in vitro models, the neuroprotection of the seed extracts was a result of their antioxidant, anti-inflammatory, and anti-apoptotic properties. In the in vivo models, neuroprotection resulted from the antioxidant and anti-inflammatory properties, a decrease in motor deficits, an improvement in learning and memory, as well as the increased release of neurotransmitters. The results show promise for the future of clinical research on new therapies for neurodegenerative diseases. However, the studies are still limited, which does not allow us to extrapolate the results to human beings with ND. Conclusions: Therefore, clinical trials are needed in order to prove the results of the in vitro and in vivo studies, as well as to assess the ideal, safe, and effective dose of these seed extracts in patients with neurodegenerative diseases.

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Section: Hibiscus Sabdariffamentioning

confidence: 99%

Neuroprotective Potential of Seed Extracts: Review of In Vitro and In Vivo Studies

et al. 2023

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Natural products targeting mitochondria: emerging therapeutics for age-associated neurological disorders

Liang

Currais

Soriano-Castell

et al. 2021

Pharmacology & Therapeutics

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Inhibitory Effect of Lonicera japonica Thunb. Flower Buds against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neurons

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송

2021

Korean J Acupunct

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Objectives : In this study, we investigated the neuroprotective effects of ethanol extract of Lonicera japonica flower buds (EELJ) on glutamate-induced neurotoxicity in mouse hippocampus-derived neuronal HT22 cells. Methods : After analyzing the cytoprotective effect of EELJ on glutamate in HT22 cells, the inhibitory effect of apoptosis was studied using flow cytometry. In order to analyze the antioxidant efficacy of EELJ, the levels of reactive oxygen species (ROS) and glutathione (GSH) were investigated, and the effects on the activities of superoxide dismutase (SOD) and catalase (CAT) were also analyzed. Furthermore, the effect of EELJ on the expression of apoptosis regulators such as Bax and Bcl-2 in glutamate-treated HT22 cells was investigated. Results : According the current results, pretreatment with EELJ significantly reduced glutamate-induced loss of cell viability and release of lactate dehydrogenase. EELJ also markedly attenuated glutamate-induced generation of intracellular ROS, which was associated with increased levels of GSH, and activity of SOD and CAT in glutamate-stimulated HT22 cells. In addition, EELJ was strikingly inhibited glutamate-induced apoptosis in HT22 cells. Furthermore, the expression of pro-apoptotic Bax was increased and the expression of anti-apoptotic Bcl-2 was decreased in glutamate-treated HT22 cells, while in the presence of EELJ, their expressions were maintained at the control levels. Conclusions : These findings indicate that EELJ protects glutamate-induced cytotoxicity in HT22 hippocampal neurons through antioxidant activity. Therefore, although identification of biologically active substances of EELJ and re-evaluation through animal experiments is necessary, this natural substance is a promising candidate for further research in preventing and treating oxidative stress-mediated neurodegenerative diseases.

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Procyanidin B2 3″-O-gallate Isolated from Reynoutria elliptica Prevents Glutamate-Induced HT22 Cell Death by Blocking the Accumulation of Intracellular Reactive Oxygen Species

Cited by 4 publications

References 40 publications

Neuroprotective Potential of Seed Extracts: Review of In Vitro and In Vivo Studies

Neuroprotective Potential of Seed Extracts: Review of In Vitro and In Vivo Studies

Natural products targeting mitochondria: emerging therapeutics for age-associated neurological disorders

Inhibitory Effect of Lonicera japonica Thunb. Flower Buds against Glutamate-Induced Cytotoxicity in HT22 Hippocampal Neurons

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