Prodigiosin - an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria

Demirtaş, İbrahim; Nazari, Teh Faridah; Jain, Kavish Kumar; Lim, Sheh-Hong

doi:10.7324/japs.2014.401001

Cited by 59 publications

(31 citation statements)

References 31 publications

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“…Harned (1954) also reported that no significant prodigiosin production was seen after 48 h incubation and supports present findings. Lee et al (2011), Priya et al (2013) and Ibrahim et al (2014), showed an antibacterial activity of prodigiosin against a range of pathogens by showing inhibition zone which supports the antimicrobial activity of prodigiosin. Ramina and Samira (2009) concluded that the antibacterial activity of prodigiosin is due to its ability to pass through the membrane and causes inhibition of target enzymes, such as DNA gyrase and topoisomerase IV, which are essential for the cell growth.…”

Section: Discussionsupporting

confidence: 60%

“…Prodigiosin is a secondary metabolite alkaloid with a unique tripyrrole chemical structure (Grimont et al 1977;Khanafari et al 2006) and has been shown to be associated with extracellular vesicles or present in intracellular granules (Matsuyama et al 1986;Kobayashi and Ichikawa 1991). Prodigiosin has no defined primary role in the growth and physiology of producing strains, but has been reported to have antimicrobial (Ibrahim et al 2014), antimalarial (Isaka et al 2002), immunomodulating (Giri et al 2004) and anticancer activities (Montaner et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity

2017

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In the present study, role of various physicochemical parameters influencing the production of antimicrobial pigment prodigiosin from RL2 was determined and optimized. The pigment-producing strain was isolated and based on molecular characterization (16S rRNA sequencing), was identified as RL2. The pigment produced by RL2 was characterized by thin layer chromatography (R 0.94), spectrophotometrically (λ 535 nm) and identified as prodigiosin. Optimization of production parameters of prodigiosin revealed, nutrient broth medium supplemented with lactose and yeast extract at 1% concentration each, have a positive effect on the bacterial growth (10.25-4.6 mg/ml DCW) as well as pigment production (0.46-0.6 mg/ml). Prodigiosin production (0.64 mg/ml) increases optimally after 46-48 h of incubation, at 35 °C at pH between 6 and 7 with addition of metal ions such as Uranyl acetate. An increase of 65% in prodigiosin production (0.46-0.76 mg/ml) was observed after optimizing the various production parameters than unoptimized conditions. Antimicrobial activity of the prodigiosin was also evaluated and found to be effective antimicrobial agent against bacterial pathogens including sp., sp., sp. and sp. Present study indicate that RL2 is a potent source of pigment prodigiosin which can be further explored for production of prodigiosin.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity

2017

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“…Lipophilic extracts and pigments of diverse origin have shown antimicrobial activity against pathogenic human bacteria and fungi. In this sense, several studies have been carried out with positive results with pigments derived from plants and vegetables [ 59 , 74 ]; from algae [ 75 , 76 ] and from saline and halophilic bacteria [ 21 , 23 , 77 ], but to date the reports about the applicability of such extracts as antimicrobial agents are scarce. Recent studies report a moderate inhibitory effect of the extracts from the haloarchaea Halorubrum sp.…”

Section: Resultsmentioning

confidence: 99%

“…Pigments from different kinds of marine microorganisms have shown effective antibacterial properties. Examples of this are the red pigment prodigiosin, isolated from the sponge-associated bacterium Serratia marcescens [ 21 ]; or the chlorophyll a derivatives obtained from the microalga Isochrysis galbana [ 22 ]. Recently, the antimicrobial activity of pigments from some halophilic bacteria has been tested against bacteria and fungi [ 23 ]; however, so far, the studies about the antimicrobial activity of haloarchaeal pigments or extracts thereof are scarce [ 19 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)

Gómez‐Villegas

Vigara

Vilà

et al. 2020

Biology

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The need to survive in extreme environments has furnished haloarchaea with a series of components specially adapted to work in such conditions. The possible application of these molecules in the pharmaceutical and industrial fields has received increasing attention; however, many potential bioactivities of haloarchaea are still poorly explored. In this paper, we describe the isolation and identification of two new haloarchaeal strains from the saltern ponds located in the marshlands of the Odiel River, in the southwest of Spain, as well as the in vitro assessment of their antioxidant, antimicrobial, and bioactive properties. The acetone extract obtained from the new isolated Haloarcula strain exhibited the highest antioxidant activity, while the acetone extracts from both isolated strains demonstrated a strong antimicrobial activity, especially against other halophilic microorganisms. Moreover, these extracts showed a remarkable ability to inhibit the enzyme cyclooxygenase-2 and to activate the melanogenic enzyme tyrosinase, indicating their potential against chronic inflammation and skin pigmentation disorders. Finally, the aqueous protein-rich extracts obtained from both haloarchaea exhibited an important inhibitory effect on the activity of the acetylcholinesterase enzyme, involved in the hydrolysis of cholinergic neurotransmitters and related to several neurological diseases.

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“…had the potential as antioxidant. Ibrahim et al (2014) showed that S. marcescens IBRL USM 84 pigment has the ability as antibacterial against several bacteria.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Characteristics of a red-pigmented thermotolerant bacteria (GSB-001) isolated from Gedong Songo hot spring, Bandungan, Semarang – Indonesia

Kusdiyantini¹,

Alif²,

Fuadiyah³

et al. 2019

MJM

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Aims: Pigments used as food additives for a long time and people prefer natural pigment than synthetic ones because of their safety. Microorganisms are interesting for pigment production and many of them have been used in food industry. Many research have been conducted to find out for natural pigment sources. The objective of this research was to investigate the characteristics of a red-pigmented bacteria isolated from Gedong Songo hot spring, Bandungan-Indonesia. Methodology and results: Bacterial isolates were grown on Nutrient Agar for 24 h, and the morphology of the colonies and of the cells were identified. Biochemical tests included indole, methyl red, catalase, urease tests and carbohydrate fermentation. Molecular identification was based on 16S rRNA sequence. The isolate was a rod shape and Gramnegative. Biochemical tests showed that the isolate was indole negative, catalase positive, methyl red negative and urease negative. This isolate was glucose, maltose and sucrose positive and negative for lactose. 16S rRNA sequence was BLAST and it matched with Serratia marcescens strain S823. The red pigment antioxidant activity showed the highest DPPH radical scavenging of 49.11% obtained from 48 h of incubation. Functional group of S. marcescens pigment on Fourier Transform InfraRed Spectroscopy (FTIR) displayed specific peak at 1740 cm-1 represented of C═O (carbonyl) stretching group. Conclusion, significant and impact of study: Based on morphological, biochemical and moleculer identifications, it showed that the bacteria isolated from Gedong Songo hot spring Bandungan-Indonesia had 94% homology with Serratia marcescens strain S823. Based on DPPH radical scavenging test, it demonstrated that S. marcescens had potency as an antioxidant.

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Prodigiosin - an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria

Cited by 59 publications

References 31 publications

Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity

Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity

Antioxidant, Antimicrobial, and Bioactive Potential of Two New Haloarchaeal Strains Isolated from Odiel Salterns (Southwest Spain)

Characteristics of a red-pigmented thermotolerant bacteria (GSB-001) isolated from Gedong Songo hot spring, Bandungan, Semarang – Indonesia

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