Prodigiosin derived from chromium-resistant Serratia sp. prevents inflammation and modulates gut microbiota homeostasis in DSS-induced colitis mice

Nie, Hao; Li, Yingli; Lü, Xiaoling; Yan, Jing; Xiang-ru, Liu; Yin, Qi

doi:10.1016/j.intimp.2023.109800

Cited by 9 publications

(11 citation statements)

References 32 publications

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“…Akkermansia , an extensively occurring species of the phylum Verrucomicrobia in both humans and mice, plays an essential role in sustaining the function of the intestinal barrier and in maintaining homeostasis of the immune systems. It shows a positive correlation with the level of IL-10 expression . In our study, G-EMP administration suppressed the inflammatory response and the numbers of Akkermannia and Helicobacter recovered to approximately the same as those detected in the NC group.…”

Section: Discussionsupporting

confidence: 85%

“…It shows a positive correlation with the level of IL-10 expression. 46 In our study, G-EMP administration suppressed the inflammatory response and the numbers of Akkermannia and Helicobacter recovered to approximately the same as those detected in the NC group. Furthermore, G-EMP treatment of DSS-induced colitis mice reversed DSS-mediated alterations in several microorganisms, including Lactobacillus, Bacteroides, Allobaculum, and Clostridiaceae Clostridium.…”

Section: ■ Discussionsupporting

confidence: 72%

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An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Xie,

Chen,

et al. 2024

J. Agric. Food Chem.

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Inflammatory bowel disease is a major health problem that can lead to prolonged damage to the digestive system. This study investigated the effects of an exopolysaccharide from genistein-stimulated Monascus purpureus (G-EMP) in a mouse model of colitis to clarify its molecular mechanisms and identified its structures. G-EMP (Mw = 56.4 kDa) was primarily consisted ofintervention reduced the loss of body weight, degree of colonic damage and shortening, disease activity index scores, and histopathology scores, while restoring goblet cell production and oxidative homeostasis, repairing colonic functions, and regulating inflammatory cytokines. RNA sequencing and Western blot analysis indicated that G-EMP exerts anti-inflammatory properties by suppressing the TLR4/MAPK/NF-κB inflammatory signaling pathway. G-EMP modulated the gut microbiota by improving its diversities, elevating the relative abundances of beneficial bacteria, declining the Firmicutes/Bacteroidota value, and regulating the level of short-chain fatty acids (SCFAs). Correlation analysis demonstrated strong links between SCFAs, gut microbiota, and the inflammatory response, indicating the potential of G-EMP to prevent colitis.

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Section: Discussionsupporting

confidence: 85%

Section: ■ Discussionsupporting

confidence: 72%

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Xie,

Chen,

et al. 2024

J. Agric. Food Chem.

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“…The High Performance Liquid Chromatography (HPLC) results showed that PG was present in the secondary metabolites of Serratia marcescens CM01, with both the PG standard and the crude extract having a single maximum absorption peak at 535 nm. Chromatograms were derived from the group’s initial data [ 16 , 17 ] ( Supplementary Materials ).…”

Section: Resultsmentioning

confidence: 99%

“…Relative expression levels of genes were calculated with the 2 −ΔΔct method by using reference gene GAPDH to normalize. The RT-PCR primer sequences [ 17 ] used are shown in Table 2 .…”

Section: Methodsmentioning

confidence: 99%

“…In a previous investigation, our research team isolated the Serratia marcescens CM01 strain and demonstrated its secondary metabolite prodigiosins (PG) as an effective agent in reducing drug-resistant Staphylococcus aureus biofilms [ 16 ] and modulating the intestinal immune response [ 17 ]. However, the specific impact of PG on P. aeruginosa biofilms remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Prodigiosin as an Antibiofilm Agent against the Bacterial Biofilm-Associated Infection of Pseudomonas aeruginosa

Ma,

Xiao,

et al. 2024

Pathogens

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Pseudomonas aeruginosa is known to generate bacterial biofilms that increase antibiotic resistance. With the increase of multi-drug resistance in recent years, the formulation of a new therapeutic strategy has seemed urgent. Preliminary findings show that Prodigiosin (PG), derived from chromium-resistant Serratia marcescens, exhibited efficient anti-biofilm activity against Staphylococcus aureus. However, its anti-biofilm activity against P. aeruginosa remains largely unexplored. The anti-biofilm activity of PG against three clinical single drug-resistant P. aeruginosa was evaluated using crystal violet staining, and the viability of biofilms and planktonic cells were also assessed. A model of chronic lung infection was constructed to test the in vivo antibiofilm activity of PG. The results showed that PG inhibited biofilm formation and effectively inhibited the production of pyocyanin and extracellular polysaccharides in vitro, as well as moderated the expression of interleukins (IL-1β, IL-6, IL-10) and tumor necrosis factor (TNF-α) in vivo, which might be attributed to the downregulation of biofilm-related genes such as algA, pelA, and pslM. These findings suggest that PG could be a potential treatment for drug-resistant P aeruginosa and chronic biofilm infections.

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The prophylaxis functions of Lactobacillus fermentumGLF‐217 and Lactobacillus plantarumFLP‐215 on ulcerative colitis via modulating gut microbiota of mice

Li,

Liu,

Wang

et al. 2024

J Sci Food Agric

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BackgroundThe strong connection between gut microbes and human health has been confirmed by an increasing number of studies. Although probiotics have been found to relieve ulcerative colitis, the mechanism varies by the species involved. In this study, the physiological, immune and pathological factors of mice were measured and shotgun metagenomic was conducted to investigate the potential mechanisms in preventing ulcerative colitis.ResultsThe results demonstrated that ingestion of Lactobacillus fermentum GLF‐217 and Lactobacillus plantarum FLP‐215 significantly alleviated ulcerative colitis induced by dextran sulfate sodium (DSS), as evidenced by the increase in body weight, food intake, water intake and colon length as well as the decreased in disease activity index, histopathological score and inflammatory factor. Both strains not only improved intestinal mucosa by increasing mucin‐2 and zonula occludens‐1, but also improved the immune system response by elevating interleukin‐10 levels and decreasing the levels of interleukin‐1β, interleukin‐6, tumor necrosis factor‐α and interferon‐γ. Moreover, L. fermentum GLF‐217 and L. plantarum FLP‐215 play a role in preventing DSS‐induced colitis by regulating the structure of gut microbiota and promoting the formation of short‐chain fatty acids.ConclusionsThis study may provide reference for the prevention strategy of ulcerative colitis.This article is protected by copyright. All rights reserved.

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Prodigiosin derived from chromium-resistant Serratia sp. prevents inflammation and modulates gut microbiota homeostasis in DSS-induced colitis mice

Cited by 9 publications

References 32 publications

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

An Exopolysaccharide from Genistein-Stimulated Monascus Purpureus: Structural Characterization and Protective Effects against DSS-Induced Intestinal Barrier Injury Associated with the Gut Microbiota-Modulated Short-Chain Fatty Acid-TLR4/MAPK/NF-κB Cascade Response

Prodigiosin as an Antibiofilm Agent against the Bacterial Biofilm-Associated Infection of Pseudomonas aeruginosa

The prophylaxis functions of Lactobacillus fermentumGLF‐217 and Lactobacillus plantarumFLP‐215 on ulcerative colitis via modulating gut microbiota of mice

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