Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis

Ali, Randa H.; Ali, Mohamed; Samir, Reham

doi:10.3390/vaccines11010037

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Serum sample from animal groups were collected at the end of immunization and challenge experiment (24 h post-challenge). The procedure for determining serum bactericidal activity (SBA) is outlined in [ 16 , 56 , 57 ]. In short, 100 µL of S. flexneri 2b serotype ATCC 12022 adjusted at (1 × 10 6 CFU/mL, OD 600 :0.2) and added to 25 µL of sera from immunized and non-immunized challenged groups.…”

Section: Methodsmentioning

confidence: 99%

Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype

Abdelfattah,

Samir,

Amin

2023

Gut Pathog

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Background Bacterial ghost cells (BGCs) are cells were drained of their genetic and cytoplasmic components. This work aimed to develop vaccine candidates against the Shigella flexneri (S. flexneri) 2b serotype using the BGCs approach. For the first time, (S. flexneri) 2b serotype BGCs vaccine was prepared by incubation with Triton X-100 (TX100) for only 12 h. Its safety and immunogenicity were compared to another vaccine produced using a previously used surfactant, namely Tween 80 (TW80). Scanning electron microscopy (SEM), cellular DNA, protein contents measurements, and ghost cell re-cultivation were used to confirm the successful generation of the BGCs. Immunogenicity was assessed through mice's intraperitoneal (IP) immunization followed by infection with S. flexneri ATCC 12022. Finally, histopathological examination was carried out. Results Viable colony forming units (CFUs) of S. flexneri were counted from stool samples as well as homogenized colon tissues of the non-immunized challenged group. Immunized mice sera showed a significant increase in serum bactericidal activity of both preparations (TX100 = 40% and TW80 = 56%) compared to the non-immunized challenged group (positive control). The IgG levels of the bacterial ghost-vaccinated groups were four and three times greater for the TX100 and TW80 ghost vaccines, respectively, compared to that of the positive control; both bacterial ghost vaccines (BGVs) were safe and effective, according to the results of the safety check tests and histopathological analysis. Conclusions When comparing the BGVs prepared using TX100 and TW80 methods, the use of TX100 as a new chemical treating agent for BGC production attained robust results in terms of shorter incubation time with the targeted cells and a strong immune response against S. flexneri 2b serotype ATCC 12022 in the IP challenge test. However, a clinical study is needed to confirm the efficacy and total safety of this novel vaccine.

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Section: Methodsmentioning

confidence: 99%

Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype

Abdelfattah,

Samir,

Amin

2023

Gut Pathog

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“…Vaccination has been recommended as the best approach to avoid infections from intracellular infectious pathogens [6]. The bacterial-ghost approach may provide a novel unconventional way to improve such a vaccine [7]. Bacterial ghosts (BGs) have been established against various microbial pathogens [8].…”

Section: Introductionmentioning

confidence: 99%

Production and Evaluation of the Immunogenicity of an Enterobacter aerogenes Ghost Vaccine in a Mouse Model

Zahran

Hassan²,

Keshta³

et al. 2023

Egypt. J. Chem.

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Enterobacter aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections. Multidrug-resistant strains have emerged as a public health threat. In this study, we used a non-expensive method called the sponge-like reduced protocol (SLRP) to create E. aerogenes bacterial ghosts EAGs (deactivated bacteria) and evaluated their safety and immunogenicity in mice. The mice were given three doses of EAGs or EAGs with an adjuvant and then challenged with live E. aerogenes bacteria. The results showed that the EAG-immunized mice had higher levels of specific antibodies IgG, IgA, and IgM, lower bacterial load in internal organs after challenge, and improved histopathological examination of the liver and spleen compared to a negative control group. The serum from the EAG-immunized mice also had cross-reactivity with other Enterobacteriaceae pathogens using western blotting and enzyme-linked immunosorbent assay (ELISA). These findings suggest that the EAG vaccine may be an effective and safe alternative for the prevention and control of multidrug-resistant infections caused by E. aerogenes. This study showed that E. aerogenes ghosts could potentially be used as a vaccine.

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A Simple and Rapid Method of Probiotic Bacterial Ghost Cell Preparation to Deliver Mycobacterium tuberculosis Antigen

Aarthi,

Anjana,

Tejal

et al. 2024

Mol Biotechnol

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Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis

Cited by 6 publications

References 27 publications

Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype

Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype

Production and Evaluation of the Immunogenicity of an Enterobacter aerogenes Ghost Vaccine in a Mouse Model

A Simple and Rapid Method of Probiotic Bacterial Ghost Cell Preparation to Deliver Mycobacterium tuberculosis Antigen

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