Production and characterization of chicken blood hydrolysate with antihypertensive properties

Abstract

Chicken blood has limited utilization despite its high protein content. Production of a blood hydrolysate exhibiting angiotensin I-converting enzyme ( ACE )–inhibitory activity would be means of valorizing chicken blood. The optimized conditions used to produce chicken blood corpuscle hydrolysate ( BCH ) by Alcalase were 51.1°C, 4% enzyme, and pH 9.6 for 6 h, resulting in a 35.8% degree of hydrolysis and 37.7% ACE inhibition at a peptide concentration of 0.2 mg/mL. The permeate of a 1-kDa membrane, BCH-III, showed a 2.5-fold increase in ACE inhibition compared with that of BCH. BCH-III was resistant to in vitro gastrointestinal digestion, whereas the BCH digesta exhibited an increased ACE-inhibitory activity after digestion. Both BCH and BCH-III were rich in hydrophobic amino acids. A single administration of BCH and BCH-III to spontaneously hypertensive rats at concentrations of 600 and 100 mg/kg, respectively, lowered the systolic blood pressure by −57.7 and −70.9 mmHg, respectively, 6 h after oral administration compared with the control group. The blood pressure–lowering effect of the 600 mg/kg BCH dose was comparable with that of the 100 mg/kg BCH-III dose after 4 wk of oral administration. Both BCH and BCH-III could be developed for use as nutraceutical products with antihypertensive effects.

Protective effects of crude chalaza hydrolysates against liver fibrogenesis via antioxidation, anti-inflammation/anti-fibrogenesis, and apoptosis promotion of damaged hepatocytes

“…Protein in eggs is considered as a good source of essential amino acids for humans. Recently, animal-origin antioxidant protein hydrolysate, peptides, and free amino acids after enzymatic hydrolyzation have been attracted more attention ( Yang et al., 2014 ; Wongngam et al., 2020 ; Chen et al., 2021 ). Chang et al.…”

Production of Protein Hydrolysate Containing Antioxidant and Angiotensin -I-Converting Enzyme (ACE) Inhibitory Activities from Tuna (Katsuwonus pelamis) Blood

“…TB has ~5-6% crude protein [3] that might be recovered and used as a highly valued product. The utilization of animal blood for bioactive hydrolysates has been reported for chicken blood hydrolysate [4,5], pig hemoglobin and blood hydrolysates [6,7] and for deer, sheep, pig and cattle red blood hydrolysate [8].…”

Association analysis and expression level of ACE polymorphisms with egg-laying trait in Taihang chicken

“…Egg-laying performance has always been an important factor limiting the development of excellent local chicken breeds in China. The egg-laying trait in chickens is a complex trait controlled by a variety of factors that are highly correlated, either promoting each other or constraining each other ( Wongngam et al, 2020 ). The generation and development of molecular genetic marker-assisted selection methods provide a good means to shorten the generation intervals and accelerate the genetic progress ( Xu et al, 2017 ).…”