Production and characterization of exopolysaccharides (EPS) from mangrove filamentous fungus, Syncephalastrum sp.

Abstract

In this study, the production and characterization of exopolysaccharide from Syncephalastrum sp. was carried out. This is the first report on the production of exopolysaccharides from Syncephalastrum sp. Totally, four different fungi (Aspergillus niger, Aspergillus flavus, Penicillium expansum and Syncephalastrum sp.) were screened for exopolysaccharide production. Among these, maximum polysaccharide producing species was selected for further large scale production. Different concentrations (6, 8 and 10%) of sucrose were also used to increase polysaccharide production. Overall results depicted that 8% of sucrose concentration exhibited higher amount of polysaccharide production. Further, structure of polysaccachride was confirmed by FT-IR and NMR spectroscopy. Based on this spectroscopy results, the polysaccharide produced by Syncephalastrum sp. was 3 linked β-D-galactopyronosyl units. The polysaccharide produced by this species composed of galactose as predominant monosccharides in the cell wall of Syncephalastrum sp. and their idealized structures were established.

Bioremoval of PVP-coated silver nanoparticles using Aspergillus niger: the role of exopolysaccharides

“…From our perspective, both fungi and their produced EPS represent a low-cost, highly efficient adsorbing material that can remove PVP-Ag-NPs from aqueous media. The macromolecular structure and net negative charge of both fungi (Viraraghavan and Srinisivan 2011) and EPS provide a suitable matrix for entrapment of pollutants and thus, it is used as flocculants (Sharmila et al 2014 ) in addition to its use as a bioadsorbent for heavy metals (Gupta and Diwan 2017 ). In the present study, EPS-induced A. niger pellets removed the majority of PVP-Ag-NPs from aqueous media as opposed to a smaller fraction when using EPS-containing media.…”

Bioremoval of PVP coated silver nanoparticles using Aspergillus niger: the role of exopolysaccharides

“…From our perspective, both fungi and their produced EPS represent a low-cost, highly e cient adhesive material that can remove PVP-Ag-NPs from aqueous media. The macromolecular structure and net negative charge of both fungi (Viraraghavan and Srinisivan 2011) and EPS provide a suitable matrix for entrapment of pollutants and thus it is used as occulants (Sharmila et al 2014) in addition to its use as a bioadsorbent for heavy metals (Gupta and Diwan 2017). Based on its nature, it was expected to remove ENPs from aqueous media.…”

Structural elucidation of the exopolysaccharide produced by Curvularia lunata isolate RJ01

“…The polysaccharide monomer units can vary, and include mannose (Chen et al 2011), glucose (Guo et al 2013), galactose (Chen et al 2010), and rhamnose (Mahapatra and Banerjee 2012), generating either homo-or hetero-polysaccharides that can contain other inorganic or organic compounds (Llamas et al 2012). Some of these polysaccharides consist of monomers linked by diverse alpha-and beta-glucosidic linkages (Sharmila et al 2014). Although some are linear, most fungal glucans have a branched (1-3)-ß-linked backbone of glucose units with branches of short glucose chains linked to the backbone by (1-6)-ß linkages in which the frequency of branching can vary (Chen and Seviour 2007;Xiao et al 2020).…”