Production and characterization of microbial biosurfactants for potential use in oil-spill remediation

Martı, Mustafa Esen; Colonna, William J.; Patra, Partha; Zhang, H.; Green, C.; Reznik, Gabriel O.; Pynn, M.; Jarrell, Kevin A.; Nyman, John A.; Somasundaran, P.; Glatz, Charles E.; Lamsal, Buddhi P.

doi:10.1016/j.enzmictec.2013.12.001

Cited by 56 publications

(31 citation statements)

References 45 publications

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“…The methods for this assay were described previously, where the toxicities of surfactin and FA‐Glu were compared . Salinity levels were comparable to those in this study, but, as will be seen, surfactant levels were lower.…”

Section: Methodssupporting

confidence: 68%

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Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

Colonna

Martı

Nyman³

et al. 2016

Env Prog and Sustain Energy

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If biosurfactants are to achieve their promise in environmental oil‐spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty‐acyl‐glutamate (FA‐Glu). This is the first report of hemolytic activity as a property of FA‐Glu. The method's potential for screening for toxicity against marine organisms is demonstrated. © 2016 American Institute of Chemical Engineers Environ Prog, 36: 505–510, 2017

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Section: Methodssupporting

confidence: 68%

“… Comparison of effects of surfactin and FA‐Glu on survival of Fundulus grandis fish larvae (previously published ) and integrity of sheep RBC (this work). The % survival of the fish larvae are an average of 3 replicates and the error bars indicate s.e.m.…”

Section: Resultsmentioning

confidence: 97%

Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

Colonna

Martı

Nyman³

et al. 2016

Env Prog and Sustain Energy

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“…Mucor (5) and Syncephalastrum (1) are pathogens found post‐harvest and affect mostly fruits (Mari et al . ). Botrytis (1), which commonly causes a disease called grey mould that leads to huge loss at harvest, is a fungus that grow at low temperatures causing problems in the storage of fruits (Nally et al .…”

Section: Resultsmentioning

confidence: 97%

Identification of fungi isolated from banana rachis and characterization of their surface activity

Méndez‐Castillo

Prieto-Correa

2017

Lett Appl Microbiol

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Biosurfactants are an alternative to petrochemical derivatives, and filamentous fungi are a promising source of these molecules. This work identified 33 strains of filamentous fungi in agroindustrial wastes. This is important because these results open the opportunity of finding new biosurfactants (hydrophobins) with unique properties. We propose the evaluation of surface tension in the supernatant as a quantitative screening to determine the production of biosurfactants from the strains of fungi.

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“…This may be related to the intrinsic effects of the scaling up, since the increase in volume promotes a consequent increase in the total surface area for complete saturation with biosurfactants, which explains this small variation observed in surface tension. When the issue is the production in large scale of biosurfactants, the use of bioreactors becomes the most promising alternative that will make the microbial production more favorable from the technical and economic points of view, compared to the limitations in relation to benchtop techniques (Marti et al, 2014; Luna et al, 2015). …”

Section: Resultsmentioning

confidence: 99%

Response Surface Methodology for Optimizing the Production of Biosurfactant by Candida tropicalis on Industrial Waste Substrates

et al. 2017

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Biosurfactant production optimization by Candida tropicalis UCP0996 was studied combining central composite rotational design (CCRD) and response surface methodology (RSM). The factors selected for optimization of the culture conditions were sugarcane molasses, corn steep liquor, waste frying oil concentrations and inoculum size. The response variables were surface tension and biosurfactant yield. All factors studied were important within the ranges investigated. The two empirical forecast models developed through RSM were found to be adequate for describing biosurfactant production with regard to surface tension (R2 = 0.99833) and biosurfactant yield (R2 = 0.98927) and a very strong, negative, linear correlation was found between the two response variables studied (r = −0.95). The maximum reduction in surface tension and the highest biosurfactant yield were 29.98 mNm−1 and 4.19 gL−1, respectively, which were simultaneously obtained under the optimum conditions of 2.5% waste frying oil, 2.5%, corn steep liquor, 2.5% molasses, and 2% inoculum size. To validate the efficiency of the statistically optimized variables, biosurfactant production was also carried out in 2 and 50 L bioreactors, with yields of 5.87 and 7.36 gL−1, respectively. Finally, the biosurfactant was applied in motor oil dispersion, reaching up to 75% dispersion. Results demonstrated that the CCRD was suitable for identifying the optimum production conditions and that the new biosurfactant is a promising dispersant for application in the oil industry.

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Production and characterization of microbial biosurfactants for potential use in oil-spill remediation

Cited by 56 publications

References 45 publications

Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative

Identification of fungi isolated from banana rachis and characterization of their surface activity

Response Surface Methodology for Optimizing the Production of Biosurfactant by Candida tropicalis on Industrial Waste Substrates

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