Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus.

Schultz, Clyde; Lidgerding, B. C.; McAllister, P.E.; Hetrick, Frank M.

doi:10.3147/jsfp.20.339

Cited by 16 publications

(9 citation statements)

References 3 publications

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“…The development of MAbs against fish viruses infecting salmonids, includ- (Schultz et al 1985, Winton et al 1988), IPN virus (Caswell-Reno et al 1986, Wolski et al 1986) and VHS virus (Lorenzen et al 1988, has already been reported. In the present study, we describe the production and characterization of the first MAb against the ISA virus, an orthomyxovirus-like virus pathogenic to Atlantic salmon.…”

Section: Discussionmentioning

confidence: 99%

Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus

Falk¹,

Namork²,

Dannevig³

1998

Dis. Aquat. Org.

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The preparation of the first monoclonal antibody (MAb) against the orthomyxovirus-like infectious salmon anaemia (ISA) virus is described. Characterization of the MAb included isotyping, enzyme-linked irnmunosorbent assay (ELISA), immunofluorescent staining of virus infected cell cultures (SHK-l cells), immunoelectron microscopy (IEM) of negatively stained virus preparations, virus neutralization assay and haemagglutination inhibition assay. The MAb reacted with ISA virus preparations both with immunofluorescent staining and in ELISA. No reactions were observed in cell cultures infected with other viruses infecting salmonids including infectious pancreatic necrosis (IPN) virus, viral haemorrhagic septicaemia (VHS) virus and infectious haematopoietic necrosis (IHN) virus. The MAb was also shown to neutralize ISA virus infection in cell cultures and to inhibit the haemagglutination reaction. IEM demonstrated binding to the surface of negatively stained ISA virions. Thus, it is concluded that the MAb binds to the haemagglutinin on the virion surface. Furthermore, using immunofluorescent staining of virus infected cell cultures, reactivity against all the 13 ISA virus strains currently available was demonstrated. Using the MAb, a simple, rapid direct irnrnunofluorescent assay for 1SA virus detection and titration In 96-well tissue culture plates was developed. Infectivity titrations by t h~s method correlated well with titration by cytopathic effects. The reliability of the assay was demonstrated by close agreement in virus infectivity titres among different assays for the same virus that were performed on the same day and on different days. A method for detection of viral ant~gen in cryosections from ISA diseased fish is also reported that may prove useful for the diagnosis and control of ISA

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Section: Discussionmentioning

confidence: 99%

Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus

Falk¹,

Namork²,

Dannevig³

1998

Dis. Aquat. Org.

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“…Many methods have been developed for detection and diagnosis of SVCV, including cell culture isolation and recognition of virions by electron microscopy, immunoassays such as immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and PCR techniques (Ahne et al, 1998(Ahne et al, , 2002Oreshkova et al, 1999;Koutná et al, 2003). Monoclonal antibodies (mAbs), as useful tools, have been used widely for development of effective diagnosis of fish viral pathogens, such as infectious hematopoietic necrosis virus (IHNV; Schultz et al, 1985), infectious pancreatic necrosis virus (IPNV; Wolski et al, 1986), viral hemorrhagic septicemia virus (VHSV; Sanz et al, 1992), epizootic hematopoietic necrosis virus (EHNV; Monini and Ruggeri, 2002), and so on. Concerning SVCV, mAbs specific against the SVCV had been recently developed and applied to detect the viral antigens by sandwich ELISA (Bing et al, 2007;Reschova et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of monoclonal antibodies to spring viraemia of carp virus

Chen

et al. 2008

Veterinary Immunology and Immunopathology

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“…McAllister et al (1974) and Hill et al (1975) used polyclonal rabbit antisera to determine that IHNV was serologically different from other fish rhabdoviruses although slight cross reactions were noted. Schultz et al (1985) developed the first monoclonal antibody against IHNV. Although :he antibody had little neutralizing activity, it reacted with IHNV (as determined by enzyme-linked immunosorbent assay) but did not recognize any of the 3 serotypes of viral hemorrhagic septicemia virus tested.…”

Section: Introductionmentioning

confidence: 99%

Neutralizing monoclonal antibodies recognize antigenic variants among isolates of infectious hematopoietic necrosis virus

Winton¹,

Arakawa²,

Lannan³

et al. 1988

Dis. Aquat. Org.

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Neutralizing monoclonal antibodies were developed against strains of infectious hematopoietic necrosis virus (IHNV) from steelhead trout Salmo gajrdneri in the Deschutes River of Oregon, chinook salmon Oncorhynchus tshawytscha in the Sacramento h v e r of California, and rainbow trout Salmo gairdneri reared in the Hagerman Valley of Idaho. USA. These antibodies were tested for neutralization of 12 IHNV isolates obtained from salmonids in Japan, Alaska, Washington, Oregon, California, and Idaho. The antibodies recognized antigenic variants among the isolates and could be used to separate the viruses into 4 groups. The members of each group tended to be related by geographic area rather than by source host species, virulence, or date of isolation.

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Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus.

Cited by 16 publications

References 3 publications

Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus

Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus

Development and characterization of monoclonal antibodies to spring viraemia of carp virus

Neutralizing monoclonal antibodies recognize antigenic variants among isolates of infectious hematopoietic necrosis virus

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