Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

Adıgüzel, Ali Osman; Tunçer, Münir

doi:10.15376/biores.12.2.2528-2547

Cited by 16 publications

(6 citation statements)

References 35 publications

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“…Tryptone was also found to be the best organic nitrogen source for xylanase production with Bacillus subtilis sp. BS04 [ 11 ] and Geobacillus thermolevarans [ 35 ], however, yeast extract was preferred nitrogen source for xylanase production by Actinomadura geliboluensis [ 36 ], and beef extract for Bacillus pumilis SV-85S [ 7 ]. The corn steep liquor, a byproduct from wet corn milling, contains residual sugars from refining, and can be a good source of nitrogen, amino acids, vitamins, and minerals.…”

Section: Resultsmentioning

confidence: 99%

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

2018

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The aim of the current study was to optimize the production of xylanase, and its application for ethanol production using the lignocellulosic biomass. A highly thermostable crude xylanase was obtained from the Geobacillus sp. strain DUSELR13 isolated from the deep biosphere of Homestake gold mine, Lead, SD. Geobacillus sp. strain DUSELR13 produced 6 U/mL of the xylanase with the beechwood xylan. The xylanase production was improved following the optimization studies, with one factor at a time approach, from 6 U/mL to 19.8 U/mL with xylan. The statistical optimization with response surface methodology further increased the production to 31 U/mL. The characterization studies revealed that the crude xylanase complex had an optimum pH of 7.0, with a broad pH range of 5.0–9.0, and an optimum temperature of 75 °C. The ~45 kDa xylanase protein was highly thermostable with t1/2 of 48, 38, and 13 days at 50, 60, and 70 °C, respectively. The xylanase activity increased with the addition of Cu+2, Zn+2, K+, and Fe+2 at 1 mM concentration, and Ca+2, Zn+2, Mg+2, and Na+ at 10 mM concentration. The comparative analysis of the crude xylanase against its commercial counterpart Novozymes Cellic HTec and Dupont, Accellerase XY, showed that it performed better at higher temperature, hydrolyzing 65.4% of the beechwood at 75 °C. The DUSEL R13 showed the mettle to hydrolyze, and utilize the pretreated, and untreated lignocellulosic biomass: prairie cord grass (PCG), and corn stover (CS) as the substrate, and gave a maximum yield of 20.5 U/mL with the untreated PCG. When grown in co-culture with Geobacillus thermoglucosidasius, it produced 3.53 and 3.72 g/L ethanol, respectively with PCG, and CS. With these characteristics the xylanase under study could be an industrial success for the high temperature bioprocesses.

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Section: Resultsmentioning

confidence: 99%

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

2018

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“…The media were supplemented with 50 g/L of rural agro-industrial wastes with approximately particle size of 1 mm (mesh size-20). The primary carbon source from rural agroindustrial wastes was sugarcane bagasse (50 g/L), corncob (50 g/L), rice husk (50 g/L), and sawdust (50 g/L) obtained from Davangere, Karnataka, India [26,27]. All these components were cleaned using tap water and distilled water followed by overnight drying in a hot air oven at 60°C.…”

Section: Determination Of Carbon Source For 14-β-xase Production From...mentioning

confidence: 99%

“…One unit of activity of 1,4-β-Xase is defined as, enzyme amount necessary to release 1 μmol equivalent xylose for each minute per mL at the standard conditions. Bradford assay (bovine serum albumin -standard protein) was used to examine the protein content in crude enzyme [27].…”

Section: Determination Of Enzyme Activitymentioning

confidence: 99%

“…The maximum protein content was examined in sugarcane bagasse (1.487 mg/mL) compared to corn cob (1.381 mg/mL), rice straw (1.462 mg/mL), sawdust (1.154 mg/mL), and beechwood xylan (1.2 mg/mL), respectively [Figure 4]. Since, the hemicelluloses and protein content in sugarcane bagasse was higher, which results in the higher production of 1,4-β-Xase compared to other microorganisms [27,32]. Thus, sugarcane bagasse is an alternative for standard substrate for the scale-up of value added 1,4-β-Xase product production.…”

Section: Selection Of Carbon Source As Natural Substratementioning

confidence: 99%

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Fermentation medium optimization for the 1,4-ß-Endoxylanase production from Bacillus pumilus using agro-industrial waste

Savanth¹,

Gowrishankar²,

Roopa³

2022

J App Biol Biotech

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Endo-1,4-β-xylanase (1,4-β-Xase) hydrolyzes hemicelluloses to xylose by the breakdown of linear polysaccharide chain β-1,4-xylan. The commercial applications of the enzyme include paper and pulp, bread and beverages, and ethanol production. The study aimed at optimizing the 1,4-β-Xase production of Bacillus pumilus by submerged fermentation using Plackett-Burman (P-B) design and central composite design (CCD). P-B design was used to screen the biotic variables and screened yeast extract, ferrous sulfate, manganese sulfate, and sugarcane bagasse as significant variables contributing the most to 1,4-β-Xase production. The R 2 value of the model was 1.0000 which indicated that the model is good. The screened four variables were optimized with CCD to study the interaction followed by optimization of abiotic variables (temperature and pH). Through CCD, yeast extract (10 g/L), ferrous sulfate (0.5 g/L), manganese sulfate (0.1 g/L), and sugarcane bagasse (50 g/L), temperature (37.5°C), and pH (7) were found to be the most significant factors affecting the 1,4-β-Xase production of B. pumilus. Our studies indicate the probability of highest production of 1,4-β-Xase on naturally available agro-waste sugarcane bagasse, which can be an alternative to standard substrate beechwood xylan for the production of 1,4-β-Xase.

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“…The results indicated that endo-cellulase from Streptomyces argenteolus AE58P yielded 76% of glucose when biomass was pretreated with Accellerase 1500. Adiguzel and Tuncer (2017) produced endoglucanase and endoxylanase from Actinomadura geliboluensis, partially purified the enzymes and applied their mixture for saccharification of alkali-pretreated wheat straw. They achieved production of 265.12 mg/g sugars using the cocktail of hemicellulolytic and cellulolytic enzyme from the actinomycete.…”

Section: Biomass Saccharificationmentioning

confidence: 99%

Saccharification of Parthenium hysterophorus biomass using cellulase from Streptomyces sp. NAA2

Saini

Aggarwal

2019

Ann Microbiol

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Parthenium hysterophorus biomass can be used as a non-conventional renewable feedstock for the production of bioethanol. Therefore, the present work was designed to hydrolyze P. hysterophorus biomass using cellulase enzyme produced from an actinomycete, i.e., Streptomyces sp. NAA2 using P. hysterophorus biomass as a substrate. The isolate NAA2 was identified by molecular characterization of 16SrDNA. The enzyme production by strain NAA2 was enhanced by optimization studies conducted under submerged fermentation conditions using P. hysterophorus as a substrate. The crude enzyme produced under optimized conditions was used to hydrolyze alkali-acid pretreated P. hysterophorus biomass. The highest CMCase production was achieved in 4-5 days when steam-pretreated P. hysterophorus biomass was used at 1% (w/v) concentration, using 2 discs (1 disc = 5 × 10 7 spores/ml) of inoculum, an initial pH 6.5, temperature at 40°C, an agitation speed of 120-150 rpm, and by supplementing fermentation medium with 1.5% (w/v) carboxymethyl cellulose (CMC) as additional carbon source. Under optimized conditions, the actinomycete strain NAA2 showed production of 0.967 ± 0.016 U/ml CMCase, 0.116 ± 0.08 FPU/ ml FPase, and 0.22 ± 0.012 U/ml β-glucosidase enzymes. On utilizing the cellulase enzyme for biomass hydrolysis, maximum 18.2% saccharification yield (of cellulose 0.202 g/g) was achieved in 96 h when enzyme and substrate levels were 30 FPU/ 100 ml and 2% (w/v) respectively. Parthenium hysterophorus biomass can be hydrolyzed enzymatically yielding considerable amounts of total reducing sugars. It can, therefore, be used as a feedstock for the production of bioethanol. Also, it has the potential to act as a substrate for the production of cellulases. Furthermore, the improved cellulolytic potential of Streptomyces sp. NAA2 can be exploited in various industrial applications.

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Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

Cited by 16 publications

References 35 publications

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass

Fermentation medium optimization for the 1,4-ß-Endoxylanase production from Bacillus pumilus using agro-industrial waste

Saccharification of Parthenium hysterophorus biomass using cellulase from Streptomyces sp. NAA2

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