Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay

Ward, C. M.; Wilkinson, A. W.; Bramham, Sara; Lee, H. A.; Chan, Henry; Butcher, G. W.; Hutchings, Amanda; Morgan, Michael R. A.

doi:10.1007/bf03192146

Cited by 21 publications

(11 citation statements)

References 24 publications

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“…Booster injections have been given normally 3 days prior to removal of spleen for fusion ( Candlish et al . 1990 ; Ward et al . 1990 ).…”

Section: Discussionmentioning

confidence: 99%

“…© 1999 The Society for Applied Microbiology ficity do not vary from bleed to bleed and additionally, unlike polyclonal antibodies, do not react with other aflatoxin analogues. Most of the reports that have appeared so far on the production of monoclonal antibodies for aflatoxins have involved standard immunization protocols which include four intraperitoneal injections at weekly intervals (Candlish, Stimson and Smith 1985) or monthly intervals (Ward et al 1990) with a final immunization 3 days prior to fusion. The numbers of clones obtained in each fusion have varied from one to a maximum of seven.…”

Section: Introductionmentioning

confidence: 99%

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Production and characterization of monoclonal antibodies for aflatoxin B1

Devi¹,

Mayo²,

Reddy³

et al. 1999

Lett Appl Microbiol

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. Hybridomas that secreted antibodies for aflatoxin B1 were selected using two immunization protocols referred to as A and B. Protocol A is a standard immunization method and resulted in the selection of only two clones that produced monoclonal antibodies against aflatoxin B1. In protocol B a unique immunization schedule which resulted in the generation of 10 hybridomas is described. Of the 10, one antibody was highly specific to B1, four antibodies reacted equally strongly with B1, G1 and weakly with B2. Another four reacted strongly with B1 and weakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2. Interestingly all the 10 antibodies showed little or no cross-reaction with G2.

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“…Booster injections have been given normally 3 days prior to removal of spleen for fusion ( Candlish et al . 1990 ; Ward et al . 1990 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and characterization of monoclonal antibodies for aflatoxin B1

Devi¹,

Mayo²,

Reddy³

et al. 1999

Lett Appl Microbiol

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“…It is apparent that AFB 1 ± BSA at 125 ng/ml consistently gave a linear standard curve (Rˆ0.99) when AFB 1 standards of concentrations of 0.1± 25 ng/ml were tested. DiOE erent workers have employed diOE erent AFB 1 ± BSA concentrations (Candlish et al 1985, Ward et al 1990, Ramakrishna and Mehan 1993; presumably conditions employed for performing ELISA in¯uenced the coating concentration. Although not reported in this paper, we have noticed that two diOE erent batches of AFB 1 ± BSA from Sigma Chemical Company required diOE erent concentrations to coat the ELISA plates.…”

Section: Determination Of Optimum Conditions For Indirect Competitivementioning

confidence: 99%

Aflatoxins B₁in different grades of chillies (Capsicum annumL.) in India as determined by indirect competitive-ELISA

Reddy

Mayi²,

Reddy³

et al. 2001

Food Additives and Contaminants

125

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Samples of the three grades of chilli pod (grades 1 to 3) were collected during surveys in 1998 and 1999 from the principal market yards and cold storage facilities of the major chilli-growing areas of Andhra Pradesh (AP), India. Chilli powders were collected from different supermarkets in Hyderabad, AP. They were analysed for aflatoxin B1 (AFB1) content by an indirect competitive ELISA. To avoid the influence of interfering substances present in chilli extracts, it was necessary to prepare the aflatoxin standards in methanol extracts of chillies free from aflatoxins. For nine representative samples there was good agreement between ELISA and HPLC estimations of AFB1 and the results suggested that the ELISA procedure adopted was dependable. Of the 182 chilli samples tested, 59% of the samples were contaminated with AFB1 and 18% contained the toxin at non-permissible levels. The highest AFB1 concentration of 969 microg/kg was found in one sample representing grade 3. Overall the maximum percentage of chilli pods showing AFB1 levels higher than 30 microg/kg (non-permissible levels) was in grade 3. Chilli pods stored in refrigerated rooms showed the lowest proportion of samples containing aflatoxin. Nearly 9% of the chilli powders sold in supermarkets contained non-permissible aflatoxin levels. This report highlights the importance of using grade 1 chilli pods to minimize aflatoxin contamination.

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“…AFB 1 is linked to human hepatocellular carcinoma, and the International Agency for Research on Cancer regards it as a human carcinogen. [3] Hence, there is a requirement for a rapid and sensitive detection method for AFB 1 in food products.…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

Dunne

Daly

Baxter³

et al. 2005

Spectroscopy Letters

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Aflatoxin B 1 (AFB 1 ) is a highly toxic secondary metabolite of the fungal species Aspergillus flavus and Aspergillus parasiticus produced under certain environmental conditions. The gene encoding an AFB 1 -specific single-chain fragment variable (scFv) was isolated from a pre-immunized phage display library and used to express a monomeric and dimeric scFv, specific for AFB 1 , in Escherichia coli. The monomeric and dimeric scFv were then applied to the development of surface plasmon resonancebased inhibition immunoassays for the detection of AFB 1 . Regeneration of the sensor surface, which consisted of a CM5 chip immobilized with an AFB 1 derivative, was investigated and enabled at least 75 binding regeneration cycles. The inhibition assays developed had ranges of detection between 390 and 12,000 pg mL 21 (ppb) for the monomeric scFv and between 190 and 24,000 pg mL 21 (ppb) for the dimeric scFv, with coefficients of variation for the inter-day variability studies ranging from 1.9 -4.18% and 3 -11.53%, respectively.

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Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay

Cited by 21 publications

References 24 publications

Production and characterization of monoclonal antibodies for aflatoxin B1

Production and characterization of monoclonal antibodies for aflatoxin B1

Aflatoxins B₁in different grades of chillies (Capsicum annumL.) in India as determined by indirect competitive-ELISA

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments

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Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay

Cited by 21 publications

References 24 publications

Production and characterization of monoclonal antibodies for aflatoxin B1

Production and characterization of monoclonal antibodies for aflatoxin B1

Aflatoxins B1in different grades of chillies (Capsicum annumL.) in India as determined by indirect competitive-ELISA

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B1Using Single‐Chain Antibody Fragments

Contact Info

Product

Resources

About

Aflatoxins B₁in different grades of chillies (Capsicum annumL.) in India as determined by indirect competitive-ELISA

Surface Plasmon Resonance‐Based Immunoassay for the Detection of Aflatoxin B₁Using Single‐Chain Antibody Fragments