Production and characterization of proteases from edible mushrooms cultivated on amazonic tubers

Abstract

Proteases are important commercial enzymes, and among their numerous sources are the Basidiomycetes. The use of proteases in many industrial areas promotes the search for enzymes with new properties. The aim of this study is to produce and characterize peptidases of a biocomposite from mycelial biomass grown in Amazonic tubers. Lentinus citrinus DPUA 1535 and Pleurotus ostreatoroseus DPUA 1720 were cultivated on Dioscorea trifida, Manihot esculenta and Dioscorea alata supplemented with rice bran or manioc flour residue in different proportions. The highest proteolytic activity was determined in the crude extract from P. ostreatoroseus grown in D. alata (DA) without supplementation (142.22 U/mL). The enzymes showed optimum activities at 40°C and pH 7.0; and stability at 50°C and pH 8.0. The proteases were classified as cysteine proteases based on the effect of inhibitors used.

Lentinus Villosus Klotzsch (1833) Am 169: A Natural and Renewable Source of Alkaline Protease / Lentinus Villosus Klotzsch (1833) Am 169: Uma Fonte Natural E Renovável De Protease Alcalina

“…In the presence of EDTA, PMSF and Pepstatin the proteases maintained catalytic activity of 100%, 99.58% and 99.47%, respectively. Machado et al (2017), Martim et al (2017a) and Brito et al (2019) reported inhibition of proteolytic activity of P. ostreatoroseus Singer (1961), P. albidus and L. crinitus in 95%, 62% and 11%, respectively, in the presence of iodoacetic acid.…”