Production and Characterization of Spray-Dried of Swamp Eel (Monopterus Albus) Protein Hydrolysate Prepared by Papain

Priatni, Sri; Harimadi, Kezia; Buana, Efendi Oulan Gustav Nata; Kosasih, Wawan; Rohmatussolihat, Rohmatussolihat

doi:10.17576/jsm-2020-4903-09

Cited by 7 publications

(4 citation statements)

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“…The particle size of BH was 902±61 nm. This result was within the particle size range (100-1500 nm) of swamp eel (Monopterus albus) protein hydrolysate obtained by Priatni et al [2020]. The particle size distribution is affected by temperature and hydrolysis time.…”

Section: Particle Size Of Hydrolysatesupporting

confidence: 76%

Antioxidant Activity of Hybrid Sturgeon (Huso dauricus × Acipenser schrenckii) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides

Noman¹,

Wang²,

Zhang³

et al. 2022

Pol. J. Food Nutr. Sci.

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Protein hydrolysates could be a natural and safer source of antioxidant peptides. The purpose of this study was to optimize the hydrolysis of Huso dauricus × Acipenser schrenckii sturgeon proteins using bromelain and purify antioxidant peptides from hydrolysate. The degree of hydrolysis of 18.69% was obtained under the optimal conditions and hydrolysate had 94.76% solubility, 902 nm particle size and high antioxidant activity. The IC 50 for DPPH • and ABTS •+ scavenging activity were 3.14 and 3.81 mg/mL, respectively. The fraction of hydrolysate with a molecular weight of <1 kDa exhibited the highest antiradical activity against DPPH • with IC 50 of 2.10 mg/mL. In turn, the IC 50 of the most active fraction after the Sephadex G-15 separation was 1.77 mg/mL. The reverse phase high performance liquid chromatography (RP-HPLC) was used to purify the peptides from this fraction. The peptide with histidine, leucine and glycine (MW of 0.2955 kDa) exhibited the highest antioxidant activity (IC 50 of 1.33 mg/mL). The obtained fractions and peptides with antioxidant activity could be used as natural substitutes for synthetic antioxidants, especially in food and pharmaceuticals.

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Section: Particle Size Of Hydrolysatesupporting

confidence: 76%

Antioxidant Activity of Hybrid Sturgeon (Huso dauricus × Acipenser schrenckii) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides

Noman¹,

Wang²,

Zhang³

et al. 2022

Pol. J. Food Nutr. Sci.

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“…This DH indicates that crude bromelain derived from C could hydrolyze proteins in P. The higher the dissolved nitrogen concentration, the longer would be the incubation time. The results of this study are almost in line with those of Ovissipour et al (2010), Utami et al (2019), Lima et al (2020) and Priatni et al (2020). These workers have found that when the incubation period increases, DH also increases.…”

Section: Pangasius Peptone Characteristicssupporting

confidence: 92%

“…Proteolytic enzymes, either from animals (Lukin 2020), plants (Saptarini et al 2020), or microorganisms (Souza et al 2015, are commonly used for peptone extraction. Plants as a source of enzymes are generally considered safe (Karahalil 2020).The enzymes often used in the protein hydrolysis are papain (Priatni et al 2020) and bromelain, derived from pineapples (Golden and Smith-Marshall 2012; Mohan et al 2016). The pineapple fruit (Ananas comosus L) contains a variety of cysteine proteinases; the most prevalent being bromelain, which hydrolytically cleaves the internal peptide bonds in proteins with broad specificity (Hale et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Utilization of Waste Mixed Pangasius Fish Fillet and Pineapple Core to Produce Peptone for Lactic Acid Bacteria Growth Media

2022

Int J Vet Sci

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Over the last few decades, the quantum of research on probiotic strains has increased rapidly in most regions of the world. The use of probiotics in animal production and human nutrition can solve many problems caused by inadequate antibiotic treatment, growth promoters, disease prevention, and improved management. Market demand for probiotics is increasing worldwide, including Indonesia, where these are mainly imported. This situation renders peptone as a media component for isolation and identification of probiotics or as a media for testing bacteria in microbiological work. Pangasius fillet waste (P) and pineapple core (C) are agro-industrial wastes used as raw materials for peptone production. The purpose of this study was to see how the ratio of P to C affects the output of peptone from agro-industrial wastes for use as a component of laboratory media for the growth of microorganisms. Protein P hydrolysis was carried out directly by mixing C in various ratios (1:1, 2:1, and 3:1) with different incubation times (T), 1 day (T1), 2 days (T2), and 3 days (T3). The P: C ratio of 3:1 with an incubation time of one day (T1) was the best for peptone production, with a yield of 42.88±2.66%, the protein content of 5.68±0.13%, degree of hydrolysis of 90.16±2.28%, and 15 amino acids. The effects of commercial and modified MRS broth media containing peptone on LAB growth were non-significantly different, with an average total LAB of 9.084-9.128 log CFU/mL. In conclusion, peptone extracted from P using crude bromelain from C can be used as a nitrogen source to formulate MRS broth for LAB growth.

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“…The spectra of omega-3 concentrate and lemuru fish oil samples were identified by FTIR (Thermo Scientific, Nicolet iS5 iD5 ATR) according to a modification of Priatni et al [18] method. The samples were placed in the discs and washed with acetone containing KBr.…”

Section: Fourier Transform Infrared (Ftir) Analysismentioning

confidence: 99%

Production of omega-3 concentrate from lemuru fish (Sardinella longiceps) oil by two-step urea complexation

Kosasih

Rosmalina

Tanuwidjaja³

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Lemuru fish (Sardinella longiceps) oil is a by-product containing polyunsaturated fatty acids (PUFA) that is important for the prevention of several diseases. For daily intake, saturated fatty acids should be removed from fish oil to obtain PUFA concentrates. Various methods have been observed in producing omega-3 concentrates. The objective of this study was to produce the omega-3 concentrate from lemuru fish oil using a two-step urea complexation method and to characterize the omega-3 product. Lemuru fish oil was saponified and purified to produce fatty acids and urea was added to the fatty acids to obtain crystal complexes. In this study, crystallization was carried out at -20 °C for 24 h with an oil-urea ratio of 1:0.625, 1:1.25, and 1:2.5. The results showed that the highest content of omega-3 (ALA, DHA, EPA) was reached at a ratio of fish oil-urea of 1:0.625. Based on the FTIR spectrum analysis, omega-3 concentrate contained the specific spectrum indicated by HC=CH trans, -C=O (ester), and -C-O (acid) groups. UV-Vis spectra analysis showed that light exposure affected the stability of omega-3 concentrate, as is evident by the hypsochromic shift of the resulting spectrum.

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Production and Characterization of Spray-Dried of Swamp Eel (Monopterus Albus) Protein Hydrolysate Prepared by Papain

Cited by 7 publications

References 0 publications

Antioxidant Activity of Hybrid Sturgeon (<i>Huso dauricus</i> × <i>Acipenser schrenckii</i>) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides

Antioxidant Activity of Hybrid Sturgeon (<i>Huso dauricus</i> × <i>Acipenser schrenckii</i>) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides

Utilization of Waste Mixed Pangasius Fish Fillet and Pineapple Core to Produce Peptone for Lactic Acid Bacteria Growth Media

Production of omega-3 concentrate from lemuru fish (Sardinella longiceps) oil by two-step urea complexation

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