Production and cytomolecular identification of new wheat-perennial rye (Secale cereanum) disomic addition lines with yellow rust resistance (6R) and increased arabinoxylan and protein content (1R, 4R, 6R)

Schneider, A.; Rakszegi, Marianna; Molnár-Láng, Márta; Szakács, Éva

doi:10.1007/s00122-016-2682-6

Cited by 42 publications

(30 citation statements)

References 73 publications

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“…The disomic addition and substitution lines play bridge tools for the transfer of exogenous genes. To date, a large number of wild relatives of wheat have been used to establish disomic addition lines by hybridizing with wheat, such as Thinopyrum intermedium [31–33], Secale cereale L. [34], Dasypyrum villosum [35], and Hordeum vulgare [36–37]. Disomic addition lines could be used to locate genes on the alien chromosome and induce translocation line by Ph1b gene, gametocidal chromosome or ionizing irradiation.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Pan

et al. 2017

PLoS ONE

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Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP), a wild relative of common wheat, possesses many potentially valuable genes for wheat breeding. To transfer these genes into wheat, a series of wheat-A. cristatum derivatives have been obtained in our laboratory. In this study, a wheat-A. cristatum derivative II-3-1 was obtained, which was proven to contain a 1P (1A) disomic substitution and 2P disomic addition line with 40 wheat chromosomes and two pairs of A. cristatum chromosomes by genomic in situ hybridization (GISH) and molecular markers analysis. By further backcrossing with the wheat parent Fukuhokomugi (Fukuho) and self-fertilization, three different lines were separated from II-3-1, including wheat-A. cristatum 1P disomic addition line II-3-1a, 2P disomic addition line II-3-1b and 1P (1A) disomic substitution line II-3-1c. Because 2P addition line had been reported before, we aimed to investigate 1P disomic addition line II-3-1a and wheat-A. cristatum 1P (1A) disomic substitution line II-3-1c. Analysis of different genetic populations demonstrated that 1P chromosome harbored multiple agronomic traits, such as elevated spike length, increased tillering ability, reduced plant height and spikelet density. Besides, bristles on the glume ridges as an important morphological marker was located on 1P chromosome. Therefore, the novel 1P addition and substitution lines will be used as important genetic materials to widen the genetic resources of wheat.

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Section: Discussionmentioning

confidence: 99%

Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Pan

et al. 2017

PLoS ONE

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“…A virtual gene-order was released in 2013 10 , and a shotgun de novo genome survey of the same line became available in 2017 11 . Both resources have been rapidly adopted by researchers and breeders 12-14 , but cannot offer the same opportunities as the higher quality genome assemblies available for other Triticeae species 9, 15-19 .…”

Section: Main Textmentioning

confidence: 99%

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

Rabanus‐Wallace

Hackauf

Mascher

et al. 2019

Preprint

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We present a chromosome-scale annotated assembly of the rye (Secale cereale L. inbred line ‘Lo7’) genome, which we use to explore Triticeae genomic evolution, and rye’s superior disease and stress tolerance. The rye genome shares chromosome-level organization with other Triticeae cereals, but exhibits unique retrotransposon dynamics and structural features. Crop improvement in rye, as well as in wheat and triticale, will profit from investigations of rye gene families implicated in pathogen resistance, low temperature tolerance, and fertility control systems for hybrid breeding. We show that rye introgressions in wheat breeding panels can be characterised in high-throughput to predict the yield effects and trade-offs of rye chromatin.

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“…Our hypothesis on the recombination was further confirmed by molecular marker analyses. Due to the high transferability of SSR markers between S. cereale and S. strictum 45 www.nature.com/scientificreports www.nature.com/scientificreports/ we obtained a similar level of polymorphism (16.4%) between the 1RS loci in '179' and 'Mv Magdaléna' when DArTseq markers were used in higher coverage for genotyping (37 out of the 225 were polymorphic).…”

Section: Effects On Morphological and Quality Traits The Novel Genetmentioning

confidence: 84%

1RS arm of Secale cereanum ‘Kriszta’ confers resistance to stripe rust, improved yield components and high arabinoxylan content in wheat

Szakács

Szőke-Pázsi

Kalapos

et al. 2020

Sci Rep

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Wheat-rye T1BL.1RS translocation is widespread worldwide as the genes on 1RS arm have positive effect on stress resistance, grain yield and adaptation ability of wheat. Nowadays, the T1BL.1RS wheat cultivars have become susceptible to rust diseases because of the monophyletic ('Petkus') origin of 1RS. Here we report and discuss the production and detailed investigation of a new T1BL.1RS translocation line carrying 1RS with widened genetic base originating from Secale cereanum. Line '179' exhibited improved spike morphology traits, resistance against stripe rust and leaf rust, as well as higher tillering capacity, fertility and dietary fiber (arabynoxylan) content than the parental wheat genotype. Comparative analyses based on molecular cytogenetic methods and molecular (SSR and DArTseq) makers indicate that the 1RS arm of line '179' is a recombinant of S. cereale and S. strictum homologues, and approximately 16% of its loci were different from that of 'Petkus' origin. 162 (69.5%) 1RS-specific markers were associated with genes, including 10 markers with putative disease resistance functions and LRR domains found on the subtelomeric or pericentromeric regions of 1RS. Line '179' will facilitate the map-based cloning of the resistance genes, and it can contribute to healthy eating and a more cost-efficient wheat production. Interspecific hybridization is one of the most promising way to improve the genetic diversity of bread wheat 1. The most widely known wheat-alien introgression has been the spontaneous translocation T1BL.1RS that is traced back to the cross of wheat 'Criewener 104' × rye 'Petkus Roggen' made in Germany between 1920 and 1930 2. Its effect on morphology and baking quality of bread wheat has been investigated in numerous studies. The positive effect of this translocation on grain yield of hexaploid wheat 3,4 and durum wheat 5,6 is commonly accepted, though in some cases no such effect was shown 7,8. Association between the presence of 1RS and increase in root biomass, water use efficiency as well as harvest index was also found in glasshouse and field experiments 9,10. 1RS arm also contains genes (Pm8, Lr26, Sr31 and Yr9) providing resistance against powdery mildew (Erysiphe graminis f. sp. tritici), leaf rust (Puccinia triticina), stem rust (Puccinia graminis f. sp. tritici), and stripe or yellow rust (Puccinia striiformis f. sp. tritici), respectively 11,12 , a reason that led to the widespread utilization of the T1BL.1RS translocation in wheat improvement. However, the resistance genes Lr26, Yr9 and Pm8 are no longer effective against new virulent biotypes of the pathogens in Europe 13. Virulence to the Sr31 resistance gene has also been reported from Uganda 14 , and from Kenya, Ethiopia, Sudan, and Iran 15. Spread of the Sr31-virulent pathotype in countries where wheats carrying the T1BL.1RS chromosome are cultivated can cause serious problems for agriculture as the genetic vulnerability of T1BL.1RS cultivars is the consequence of the lack of allelic variation on the 'Petkus'-derived 1RS arm 4. ...

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Production and cytomolecular identification of new wheat-perennial rye (Secale cereanum) disomic addition lines with yellow rust resistance (6R) and increased arabinoxylan and protein content (1R, 4R, 6R)

Cited by 42 publications

References 73 publications

Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P

Chromosome-scale genome assembly provides insights into rye biology, evolution, and agronomic potential

1RS arm of Secale cereanum ‘Kriszta’ confers resistance to stripe rust, improved yield components and high arabinoxylan content in wheat

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